EFFECTS OF INCUBATION CONDITIONS ON THE IN VITRO ASSAY METHOD FOR INSULIN

1961 ◽  
Vol 39 (9) ◽  
pp. 1381-1387 ◽  
Author(s):  
D. C. Jessup ◽  
G. S. Wiberg
Keyword(s):  
Adverse Effect ◽  
Incubation Period ◽  
Incubation Time ◽  
Glucose Concentration ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
Number Of Factors

A number of factors such as incubation time, glucose concentration, and preincubation cooling affect the deposition of glycogen and the uptake of glucose by diaphragm sections in response to insulin. The greatest response occurs during the first 30 minutes of incubation but for assay purposes an incubation period of 90 minutes was recommended as it gave the greatest difference between control and treated tissues. A glucose concentration of 0.2% in the medium was found optimum. The effect of insulin at higher and lower concentrations was not as great. Preincubation cooling had an adverse effect on the response of the tissues to insulin.

AN IN VITRO ASSAY OF CORTICOTROPHIN

1955 ◽  
Vol 33 (2) ◽  
pp. 209-216 ◽  
Author(s):  
K. W. McKerns ◽  
E. Nordstrand
Keyword(s):  
Dose Response ◽  
In Vitro Assay ◽  
Assay Method ◽  
Response Curves ◽  
Vitro Assay ◽  
Rat Adrenals ◽  
Assay Design ◽  
Dose Response Curves ◽  
Gland System

The ability of corticotrophin to increase the corticoid output of rat adrenals in an isolated gland system has been developed as a useful assay method for the measurement of corticotrophin potency. The extra corticoids produced by stimulation are measured in terms of cortisone. Log dose response curves are presented for corticotrophin levels from 0.002 to 0.135 I.U./100 mgm. adrenals. A four point assay design, the precision of corticoid measurements, and the characteristics of the log dose response curves for a number of types of corticotrophin are given. With four measurements of each dose level the average lambda s/b for 20 assays was 0.209 ± 0.085 (S.D.).

AN IN VITRO ASSAY OF CORTICOTROPHIN

1955 ◽  
Vol 33 (1) ◽  
pp. 209-216
Author(s):  
K. W. McKerns ◽  
E. Nordstrand
Keyword(s):  
Dose Response ◽  
In Vitro Assay ◽  
Assay Method ◽  
Response Curves ◽  
Vitro Assay ◽  
Rat Adrenals ◽  
Assay Design ◽  
Dose Response Curves ◽  
Gland System

The ability of corticotrophin to increase the corticoid output of rat adrenals in an isolated gland system has been developed as a useful assay method for the measurement of corticotrophin potency. The extra corticoids produced by stimulation are measured in terms of cortisone. Log dose response curves are presented for corticotrophin levels from 0.002 to 0.135 I.U./100 mgm. adrenals. A four point assay design, the precision of corticoid measurements, and the characteristics of the log dose response curves for a number of types of corticotrophin are given. With four measurements of each dose level the average lambda s/b for 20 assays was 0.209 ± 0.085 (S.D.).

Phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits

2014 ◽  
Vol 153 ◽  
pp. 130-133 ◽  
Author(s):  
Gema Flores ◽  
Fernando De la Peña Moreno ◽  
Gracia Patricia Blanch ◽  
Maria Luisa Ruiz del Castillo
Keyword(s):  
Enzyme Activity ◽  
Phenylalanine Ammonia Lyase ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
Flavonol Synthase ◽  
Berry Fruits

Development of a Highly Sensitive In Vitro Assay Method for Biological Activity of Endotoxin Contamination in Biological Products

Biologicals ◽  
2002 ◽  
Vol 30 (2) ◽  
pp. 85-92 ◽  
Author(s):  
Akihiko Yamamoto ◽  
Masaki Ochiai ◽  
Michiyo Kataoka ◽  
Hiromi Toyoizumi ◽  
Yoshinobu Horiuchi
Keyword(s):  
Biological Activity ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
Biological Products ◽  
Highly Sensitive ◽  
Endotoxin Contamination

scholarly journals Plasmodium falciparum: Assessment of Selectivity of Action of Chloroquine, Alchornea cordifolia, Ficus polita, and Other Drugs by a Tetrazolium-Based Colorimetric Assay

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Nana Kofi Ayisi ◽  
Regina Appiah-Opong ◽  
Ben Gyan ◽  
Kwasi Bugyei ◽  
Fred Ekuban
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Incubation Time ◽  
Blood Cells ◽  
Colorimetric Assay ◽  
In Vitro Assay ◽  
Effective Concentration ◽  
Vitro Assay ◽  
Number Of Cells

A tetrazolium-based colorimetric selective assay (MTT-based CSA) was developed to assess the selectivity of antimalarial drugs. This in vitro assay, unlike all others, measures the ability of drugs to indirectly protect red blood cells (RBCs) from Plasmodium-falciparum-induced destruction. Optimum incubation time and number of cells needed were 5 days and 23×106 RBCs per well, respectively. A parasitemia range of 0.375% to 3% was found to be suitable for this assay. The MTT-based CSA determined anti-P. falciparum strain DD2 activity of chloroquine at a higher 50% effective concentration (EC50) value (21.0 μg/mL) than the isotopic microtest (10.0 μg/mL). Artesunate and oxytetracycline achieved 90% effect against DD2 with minimal or no toxicity to RBCs. Against chloroquine sensitive strain 3D7, chloroquine and Alchornea cordifolia had EC50 values of 0.025 μg/mL and 4.9 μg/mL respectively, and selective index (SI) values of >2,000 and >69.4 μg/mL, respectively.

THE BIOASSAY OF LONG-ACTING THYROID STIMULATOR: A COMPARISON OF TWO METHODS

1971 ◽  
Vol 49 (3) ◽  
pp. 487-492 ◽  
Author(s):  
JANICE M. ENSOR ◽  
PAT KENDALL-TAYLOR ◽  
D. S. MUNRO ◽  
B. R. SMITH
Keyword(s):  
Thyroid Stimulating Hormone ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
In Vitro Method ◽  
Long Acting ◽  
Stimulating Hormone

SUMMARY The McKenzie (1958) assay method and an in-vitro assay method (Brown & Munro, 1967) for the determination of long-acting thyroid stimulator have been compared. Differences in the results were not great: the in-vitro method is simpler to perform and more precise. The sensitivity of the two methods is similar. The in-vitro method, when used with antiserum to thyroid-stimulating hormone in the medium, provided a satisfactory and simple means for the assay of long-acting thyroid stimulator in the serum of thyrotoxic patients.

scholarly journals In Vitro Assay Method for Augmention of Anti-Candida Activity of Murine Bone Marrow Cells by Cytokines

2001 ◽  
Vol 75 (4) ◽  
pp. 326-332
Author(s):  
Shinichiro MOROFUJI ◽  
Shigeru ABE ◽  
Shigeru TANSHO ◽  
Yasuo ONO ◽  
Hideyo YAMAGUCHI ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
Murine Bone Marrow ◽  
Marrow Cells
Sign in / Sign up

Export Citation Format

Share Document