Influences of contamination of Aeromonas hydrophila, on quality, oxidative damage, and ultrastructure in cryopreserved sperm of the silver barb, Barbonymus gonionotus

Aquaculture ◽  
2022 ◽  
Vol 547 ◽  
pp. 737440
Author(s):  
Treerat Sooksawat ◽  
Sampan Tongnunui ◽  
Subuntith Nimrat ◽  
Verapong Vuthiphandchai
Keyword(s):  
Oxidative Damage ◽  
Aeromonas Hydrophila ◽  
Cryopreserved Sperm ◽  
Silver Barb

Influence of Aeromonas hydrophila and Pseudomonas fluorescens on motility, viability and morphometry of cryostored silver barb (Barbodes gonionotus) sperm

Cryobiology ◽  
2016 ◽  
Vol 73 (2) ◽  
pp. 140-146 ◽  
Author(s):  
Traimat Boonthai ◽  
Weerasith Khaopong ◽  
Jumlong Sangsong ◽  
Verapong Vuthiphandchai ◽  
Subuntith Nimrat
Keyword(s):  
Pseudomonas Fluorescens ◽  
Aeromonas Hydrophila ◽  
Silver Barb

scholarly journals Comparison of growth performance between cryopreserved and fresh sperm-originated fry of Barbonymus gonionotus

1970 ◽  
Vol 7 (1) ◽  
pp. 145-149 ◽  
Author(s):  
F Rahman ◽  
MRI Sarder ◽  
MA Rouf
Keyword(s):  
Growth Performance ◽  
Egg Yolk ◽  
Citrate Solution ◽  
Control Groups ◽  
Significant Difference ◽  
Cryopreserved Sperm ◽  
Survival And Growth ◽  
And Control ◽  
Silver Barb ◽  
Glass Aquarium

This experiment was conducted to compare the growth performance of silver barb fry produced from cryopreserved sperm with those produced from fresh sperm. Cryopreserved sperm used for fry production was preserved with three extenders, Alsever's solution, urea egg-yolk, egg-yolk citrate solution and one cryoprotectant, DMSO. Cryodiluents were prepared by mixing the cryoprotectants at 10% concentration of the extender (% v/v). Fry produced with fresh sperm was considered as control. For comparing the growth, 60 fry of 15 day-old for each treatment of both cryopreserved and control groups were stocked to glass aquarium (50 cm x 30 cm x 28 cm) and reared them for ten weeks. Growth of fry in terms of length and weight increment in both cryopreserved and control groups were measured weekly. The growth pattern was more or less similar for all the treatments and there was no significant difference (P>0.05) between them. The survival rate of fry produced from cryopreserved sperm was 82.3% and that from fresh sperm 88%, and there was no significant difference (P>0.05) between two groups. It is therefore, concluded that the use of cryopreserved sperm does not impair survival and growth of fry. Keywords: Silver barb; Cryopreservation; Growth; Survival DOI: 10.3329/jbau.v7i1.4977 J. Bangladesh Agril. Univ. 7(1): 145-149, 2009

The role of sodA and sodB in Aeromonas hydrophila resisting oxidative damage to survive in fish macrophages and escape for further infection

2019 ◽  
Vol 88 ◽  
pp. 489-495 ◽  
Author(s):  
Meimei Zhang ◽  
Yingxue Qin ◽  
Lixing Huang ◽  
Qingpi Yan ◽  
Leilei Mao ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Aeromonas Hydrophila

scholarly journals Evaluation of the efficacies of selected antibiotics and medicinal plants on common bacterial fish pathogens

1970 ◽  
Vol 7 (1) ◽  
pp. 163-168 ◽  
Author(s):  
T Rahman ◽  
MMR Akanda ◽  
MM Rahman ◽  
MBR Chowdhury
Keyword(s):  
Medicinal Plants ◽  
Aeromonas Hydrophila ◽  
Control Measure ◽  
Edwardsiella Tarda ◽  
Effective Control ◽  
Fish Pathogens ◽  
Different Doses ◽  
Fresh Culture ◽  
Silver Barb

An experiment was conducted to compare the efficacies of some selected antibiotics and medicinal plants against common bacterial fish pathogens viz., Aeromonas hydrophila, Pseudomonas fluorescens and Edwardsiella tarda. Four different antibiotics viz., CFCIN (ciprofloxacin), Renamycin (oxytetracycline), DT-10 (doxycicline) and Sulfatrim (sulphadiazine + trimethoprim) were exposed in different doses (100, 75, 50 and 25 ppm) to the culture of freshly isolated bacteria under the in vitro condition for sensitivity test and minimum inhibitory dose (MID) was determined. Based on in vitro results, antibiotics were applied to the experimental infection of Thai silver barb, Barbonymus gonionotus. CFCIN showed the best result with 100% recoveries of challenged fish in prolonged bath treatment. Medicinal plants were selected on the basis of previous studies. Crude extracts were prepared from various parts (leaves and bulb) of garlic, turmeric, akand and neem and four different doses were applied to the fresh culture of pathogenic isolates under the in-vitro condition to determine minimum inhibitory dose (MID). However, garlic offered the best result with 90.00 ± 2.89% recoveries of challenged fish in aquarium trial. Akand + neem, turmeric and akand showed moderate to weak recovery rates with the same dose. The present study thus showed that medicinal plants would be an effective control measure along with antibiotics against bacterial fish diseases. Keywords: Efficacy; Medicinal plants; Bacterial fish pathogens DOI: 10.3329/jbau.v7i1.4980 J. Bangladesh Agril. Univ. 7(1): 163-168, 2009

scholarly journals Cryogenic Freezing of Silver Barb (Barbonymus gonionotus) Spermatozoa for Gene Pool Conservation

2013 ◽  
Vol 20 (1-2) ◽  
pp. 117-124 ◽  
Author(s):  
MRI Sarder ◽  
F Rahman ◽  
MS Hossain ◽  
MS Samad
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Gene Pool ◽  
Egg Yolk ◽  
Gradual Reduction ◽  
Cryopreserved Sperm ◽  
Thaw Period ◽  
Silver Barb

Experiments were conducted to develop and standardize the protocols of cryopreservation of sperm of Barbonymus gonionotus. Three extenders Alsever’s solution, urea-egg-yolk and egg-yolk citrate and four cryoprotectants methanol, ethanol, DMSO and DMA were used. Cryodiluents were prepared by adding 10% cryoprotectant to 90% extender (% v/v). Milt and cryodiluents were mixed at a ratio of 1 : 9 for Alsever’s solution and 1 : 4 for urea egg-yolk and egg-yolk citrate solutions. Alsever’s solution with 10% DMSO showed the best performance and produced 78 ± 2.55% sperm motility at the post-thaw period. Urea egg-yolk and egg-yolk citrate with 10% DMSO produced 76 ± 1.87% and 74 ± 1.87% post-thaw motility respectively. When cryopreserved sperm was stored in liquid nitrogen for 130 days, a gradual reduction in motility ranging from 31.25 to 37.50% was observed. This could be happened due to frequent opening of the nitrogen dewar that could cause thawing of the sample. In breeding trials, sperm preserved with Alsever’s solution and urea egg-yolk plus 10% DMSO produced 14.28 ± 7.06% and 15.46 ± 5.50% hatching respectively. In contrast, sperm preserved with egg-yolk citrate and 10% DMSO produced poor hatching as 8.01 ± 2.15%.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16863 Progress. Agric. 20(1 & 2): 117 – 124, 2009

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