Influence of Aeromonas hydrophila and Pseudomonas fluorescens on motility, viability and morphometry of cryostored silver barb (Barbodes gonionotus) sperm

Background: The combination of some plant extracts to prevent and treat bacterial infections is gaining momentum, because of effectiveness against certain bacteria. This study aims to describe the antibacterial and immunostimulant abilities of Boesenbergia pandurata (BP), Solanum ferox (SF) and Zingiber Zerumbet (ZZ) plant extracts to treat and prevent Aeromonas hydrophila and Pseudomonas fluorescens infection on Tilapia (Oreochromis niloticus). Methods: Tilapia (initial weight 15±2 g) were injected intramuscularly (0.1 ml/fish) with a combination of A. hydrophila and P. fluorescens at a density of 1×10 5 CFU ml -1 of each bacteria. Treatment trials were performed at day 7 post-injection with each combined extract, while the prevention trial was performed by including the combined extract into the commercial diet for six and seven days prior to injection. Various extract combinations were 60 mg SF extract/kg feed with 40 mg ZZ/kg feed (SF60/ZZ40), SF50/ZZ50, BP90/SF10, and BP50/SF50. Haemato-immunological parameters were performed for four weeks. Results: In prevention trials, tilapia fed SF50/ZZ50 showed a significant increase of white and red blood cells. Similarly, significantly increased haematocrit was found in tilapia fed SF50/ZZ50 in the treatment trial but not in the prevention trial. In both trials, haemoglobin of tilapia was not affected by any combined extracts but decreased the number of bacteria. Phagocytic index, respiratory burst, lysozyme activity and survival rate of fish fed combined extracts were found significantly higher than controls. The amount of pathogenic bacteria in fish fed combined extracts was lower than the control at week 4 (P<0.05). In both trials The percentage of survival rate and relative percent survival of tilapia fed SF 50/ZZ 50, showed the optimum results compared to the other combinations. Conclusions: The combined extract in feed, especially SF50/ZZ50 has a positive effect on the tilapia's innate immune system of tilapia to treat and prevent bacterial infections.

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Efficacy of Aqueous Extract of Pistia Stratiotes (Water Lettuce) Against some Tropical Fish Bacterial

Journal of Nutrition and Food Security ◽

10.18502/jnfs.v6i3.6827 ◽

2021 ◽

Author(s):

Adelakun Kehinde Moruff ◽

Halidu Shafiu Kilishi ◽

Fajobi Enobong Aloysious ◽

Joshua Deji Abiodun

Keyword(s):

Pseudomonas Fluorescens ◽

Aqueous Extract ◽

Aeromonas Hydrophila ◽

Antibacterial Activities ◽

Salmonella Typhi ◽

Tropical Fish ◽

Pistia Stratiotes ◽

Significant Activity ◽

Water Lettuce ◽

Fish Pathogens

Background: The study was undertaken to determine the phytochemical screening and the antibacterial activities of Pistia stratiotes (water lettuce) against some clinically isolated fish pathogens. Methods: The healthy and disease free plants samples were collected from Jebba Upper Basin of River Niger and tested for their antibacterial activities and phytochemical properties. Pistia stratiotes aqueous extract was extracted using sterile distilled water. The Agar diffused methods were used to determine the antimicrobial actions of the plant against fish pathogens of Pseudomonas fluorescens, Escherichia coli, Salmonella typhi, Aeromonas hydrophila, and Vibrio anguillarum. Results: The antibacterial investigation of aqueous extracts revealed the significant activity against these bacterial. Moreover, phytochemical examination of the crude extract showed the occurrence of saponins, alkaloids, tannins, anthraquinones, flavonoids, and catechins, which completely inhibited the growth of V. anguillarum and E. coli. However, Salmonella typhi, Pseudomonas fluorescens, and Aeromonas hydrophila exhibited moderate varying levels of resistance. Conclusion: The finding of this study showed that bioactive resistance from Pistia stratiotes can help disrupt the activities of various fish bacterial in aquaculture practices

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Gene expression and histopathological changes of Nile tilapia (Oreochromis niloticus) infected with Aeromonas hydrophila and Pseudomonas fluorescens

Aquaculture ◽

10.1016/j.aquaculture.2020.735392 ◽

2020 ◽

Vol 526 ◽

pp. 735392 ◽

Cited By ~ 1

Author(s):

Ahmed M. Hal ◽

Manal I. El-Barbary

Keyword(s):

Gene Expression ◽

Pseudomonas Fluorescens ◽

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Histopathological Changes ◽

Nile Tilapia Oreochromis Niloticus

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Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus)

F1000Research ◽

10.12688/f1000research.16902.1 ◽

2018 ◽

Vol 7 ◽

pp. 1847 ◽

Cited By ~ 2

Author(s):

Esti Handayani Hardi ◽

Rudy Agung Nugroho ◽

Irawan Wijaya Kusuma ◽

Wiwin Suwinarti ◽

Agung Sudaryono ◽

...

Keyword(s):

Immune System ◽

Pseudomonas Fluorescens ◽

Plant Extracts ◽

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Bacterial Infections ◽

Pathogenic Bacteria ◽

Prevention Trial ◽

Immunological Parameters ◽

Prevention Trials

Background: This study aims to describe the antibacterial and immunostimulant abilities of Boesenbergia pandurata (BP), Solanum ferox (SF) and Zingiber Zerumbet (ZZ) plant extracts to treat and prevent Aeromonas hydrophila and Pseudomonas fluorescens infection on Tilapia (Oreochromis niloticus). Methods: Tilapia (initial weight 15±2 g) were injected intramuscularly (0.1 ml/fish) with a combination of A. hydrophila and P. fluorescens at a density of 1×105 CFU ml-1 of each bacteria. Treatment trials were performed at day 7 post-injection with each combined extract, while the prevention trial was performed by including the combined extract into the diet for six and seven days prior to injection. Various combinations of extract—60 ml SF extract/kg feed with 40 ml ZZ/kg feed (SF60/ZZ40), SF50/ZZ50, BP90/SF10, and BP50/SF50—were mixed with a commercial diet and used in both treatment and prevention trials. Haematological and immunological parameters were performed every week for four weeks. Results: In prevention trials, tilapia fed SF50/ZZ50 showed a significant increase of white and red blood cells from weeks 2 to 4. Similarly, significantly increased haematocrit was also found in tilapia fed SF50/ZZ50 in the treatment trial but not in the prevention trial. However, haemoglobin of tilapia in both trials was not affected by any of the various combinations of extract in the diet. Furthermore, phagocytic, respiratory burst, lysozyme activity indexes and survival rate of fish fed with combined extracts were found to be significantly higher than controls. Moreover, the amount of pathogenic bacteria in fish that were fed combined extracts was also lower than the control and was significantly different at week 4. Conclusions: This study indicates that the addition of combined extract into feed has a positive effect on the tilapia's immune system. The SF50/ZZ50 combination appears to improve the innate immune system of tilapia to treat and prevent bacterial infections.

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