The formation of hybrid fish derived from hybridization of Megalobrama amblycephala (♀) × Siniperca chuatsi (♂)

Aquaculture ◽  
2022 ◽  
Vol 548 ◽  
pp. 737547
Author(s):  
Yude Wang ◽  
Jiajun Yao ◽  
Anmin Liao ◽  
Huifang Tan ◽  
Yaxin Luo ◽  
...  
Keyword(s):  
Megalobrama Amblycephala ◽  
Siniperca Chuatsi ◽  
Hybrid Fish

scholarly journals Asymmetric expression of homoeologous genes contributes to dietary adaption of an allodiploid hybrid fish derived from Megalobrama amblycephala (♀) × Culter alburnus (♂)

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Wuhui Li ◽  
Shi Wang ◽  
Jie Hu ◽  
Chenchen Tang ◽  
Chang Wu ◽  
...  
Keyword(s):  
Differential Expression ◽  
Megalobrama Amblycephala ◽  
Genetic Breeding ◽  
Dietary Adaptation ◽  
Homoeologous Genes ◽  
Hybrid Fish ◽  
Culter Alburnus ◽  
Mechanistic Basis ◽  
Fish Genetic Breeding ◽  
Asymmetric Expression

Abstract Background Hybridization, which can quickly merge two or more divergent genomes and form new allopolyploids, is an important technique in fish genetic breeding. However, the merged subgenomes must adjust and coexist with one another in a single nucleus, which may cause subgenome interaction and dominance at the gene expression level and has been observed in some allopolyploid plants. In our previous studies, newly formed allodiploid hybrid fish derived from herbivorous Megalobrama amblycephala (♀) × carnivorous Culter alburnus (♂) had herbivorous characteristic. It is thus interesting to further characterize whether the subgenome interaction and dominance derive dietary adaptation of this hybrid fish. Results Differential expression, homoeolog expression silencing and bias were investigated in the hybrid fish after 70 days of adaptation to carnivorous and herbivorous diets. A total of 2.65 × 108 clean reads (74.06 Gb) from the liver and intestinal transcriptomes were mapped to the two parent genomes based on specific SNPs. A total of 2538 and 4385 differentially expressed homoeologous genes (DEHs) were identified in the liver and intestinal tissues between the two groups of fish, respectively, and these DEHs were highly enriched in fat digestion and carbon metabolism, amino acid metabolism and steroid biosynthesis. Furthermore, subgenome dominance were observed in tissues, with paternal subgenome was more dominant than maternal subgenome. Moreover, subgenome expression dominance controlled functional pathways in metabolism, disease, cellular processes, environment and genetic information processing during the two dietary adaptation processes. In addition, few but sturdy villi in the intestine, significant fat accumulation and a higher concentration of malondialdehyde in the liver were observed in fish fed carnivorous diet compared with fish fed herbivorous diet. Conclusions Our results indicated that diet drives phenotypic and genetic variation, and the asymmetric expression of homoeologous genes (including differential expression, expression silencing and bias) may play key roles in dietary adaptation of hybrid fish. Subgenome expression dominance may contribute to uncovering the mechanistic basis of heterosis and also provide perspectives for fish genetic breeding and application.

scholarly journals Chimeric Genes Revealed in the Polyploidy Fish Hybrids of Carassius cuvieri (Female) × Megalobrama amblycephala (Male)

2016 ◽  
Author(s):  
Fangzhou Hu ◽  
Chang Wu ◽  
Yunfan Zhou ◽  
Shi Wang ◽  
Jun Xiao ◽  
...  
Keyword(s):  
Phenotypic Diversity ◽  
Orthologous Gene ◽  
Megalobrama Amblycephala ◽  
K Channel ◽  
The Novel ◽  
Distant Hybridization ◽  
Chimeric Genes ◽  
Hybrid Fish ◽  
Fish Hybrids ◽  
Phenotypic Novelty

AbstractThe genomes of newly formed natural or artificial polyploids may experience rapid gene loss and genome restructuring. In this study, we obtained tetraploid hybrids (4n=148, 4nJB) and triploid hybrids (3n=124, 3nJB) derived from the hybridization of two different subfamily species Carassius cuvieri (♀, 2n = 100, JCC) and Megalobrama amblycephala (♂, 2n = 48, BSB). Some significant morphological and physiological differences were detected in the polyploidy hybrids compared with their parents. To reveal the molecular traits of the polyploids, we compared the liver transcriptomes of 4nJB, 3nJB and their parents. The results indicated high proportion chimeric genes (31 > %) and mutated orthologous genes (17 > %) both in 4nJB and 3nJB. We classified 10 gene patterns within three categories in 4nJB and 3nJB orthologous gene, and characterized 30 randomly chosen genes using genomic DNA to confirm the chimera or mutant. Moreover, we mapped chimeric genes involved pathways and discussed that the phenotypic novelty of the hybrids may relate to some chimeric genes. For example, we found there is an intragenic insertion in the K+ channel kcnk5b, which may be related to the novel presence of the barbels in 4nJB. Our results indicated that the genomes of newly formed polyploids experienced rapid restructuring post-polyploidization, which may results in the phenotypic and phenotypic changes among the polyploidy hybrid offspring. The formation of the 4nJB and 3nJB provided new insights into the genotypic and phenotypic diversity of hybrid fish resulting from distant hybridization between subfamilies.

Novel insights into the immune regulatory effects of Megalobrama amblycephala intelectin on the phagocytosis and killing activity of macrophages

2021 ◽  
Vol 137 ◽  
pp. 145-154
Author(s):  
Yancui Zheng ◽  
Yunlong Liu ◽  
Hongping Li ◽  
Xu Wang ◽  
Minying Zhang ◽  
...  
Keyword(s):  
Megalobrama Amblycephala ◽  
Killing Activity ◽  
Regulatory Effects
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