Behavioural responses of Gammarus pulex (Crustacea, Amphipoda) to low concentrations of pharmaceuticals

2006 ◽  
Vol 78 (3) ◽  
pp. 209-216 ◽  
Author(s):  
H.J. De Lange ◽  
W. Noordoven ◽  
A.J. Murk ◽  
M. Lürling ◽  
E.T.H.M. Peeters
Keyword(s):  
Gammarus Pulex ◽  
Behavioural Responses ◽  
Low Concentrations

Electroantennogram, behavioural responses, and field trapping of Trypophloeus klimeschi (Coleoptera: Curculionidae: Scolytinae) to eight host volatiles

2019 ◽  
Vol 151 (02) ◽  
pp. 236-250
Author(s):  
Guanqun Gao ◽  
Lulu Dai ◽  
Jing Gao ◽  
Jiaxing Wang ◽  
Hui Chen
Keyword(s):  
Plant Volatiles ◽  
Field Trapping ◽  
Methyl Benzoate ◽  
Management Tool ◽  
Populus Alba ◽  
Host Volatiles ◽  
Behavioural Responses ◽  
Plant Volatile ◽  
Low Concentrations ◽  
Plant Volatile Compounds

AbstractTrypophloeus klimeschiEggers (Coleoptera: Curculionidae: Scolytinae) was first discovered in China in 2003, and it exhibits strong species specificity toPopulus albavar.pyramidalisBunge (Salicaceae). To screen plant volatile compounds for monitoring and trappingT. klimeschi, the electroantennogram responses of adultT. klimeschito eight plant volatiles, including nonanal, 2-methylbutanal, decanal, 2-hydroxybenzaldehyde, (Z)-3-hexen-1-ol benzoate, methyl benzoate, methyl salicylate, and geraniol were tested at various concentrations. Behavioural responses of female and male adults to various concentrations of these eight plant volatiles were also determined using a Y-tube olfactometer. We then tested the effectiveness of these compounds as lures for trappingT. klimeschiin the field. Electroantennogram tests showed thatT. klimeschipossesses olfactory sensitivity for eight compounds. Additionally, walkingT. klimeschiexhibited attraction to low concentrations (≤ 1 μg/μL) of all eight compounds in Y-tube olfactometer. Field experiment results indicated that baits composed of each volatile compound alone were more attractive to greater numbers ofT.klimeschithan the control. The methyl benzoate bait was better attracted byT.klimeschithan other tested volatiles. These results suggest that these compounds could be used in attraction of this stem-boring pest. This study could have important implications for the development of an effective semiochemical-based management tool forT. klimeschiin the field.

Biological Effects of Refinery Effluents

1973 ◽  
Vol 1973 (1) ◽  
pp. 715-723 ◽  
Author(s):  
Jenifer M. Baker
Keyword(s):  
Biological Effects ◽  
Toxic Effects ◽  
Biological Damage ◽  
Behavioural Responses ◽  
Low Concentrations ◽  
Laboratory Results ◽  
To Receive

ABSTRACT Refinery effluents contribute an estimated 300,000 tons of oil per year to the sea, but the biological effects of continuous but low concentrations of oil and other compounds are not well known. Different approaches to the problem are described, and field and laboratory results summarized. Different ecosystems differ in their capacity to receive and degrade effluents, and speed of dispersion and dilution is a major factor determining amount of biological damage. Changes in distribution and abundance of species are often very localised and in some cases may result from behavioural responses rather than direct toxic effects; areas of influence in terms of sub-lethal absorption of oil or other effluent constituents are not yet known. Biologically acceptable limits for effluents will vary considerably between sites and do not necessarily correspond with the present somewhat arbitrary restrictions.

Physiological and behavioural responses of Gammarus pulex (Crustacea: Amphipoda) exposed to cadmium

2008 ◽  
Vol 86 (3) ◽  
pp. 413-425 ◽  
Author(s):  
V. Felten ◽  
G. Charmantier ◽  
R. Mons ◽  
A. Geffard ◽  
P. Rousselle ◽  
...  
Keyword(s):  
Gammarus Pulex ◽  
Behavioural Responses

Single and combined effects of cadmium and arsenate in Gammarus pulex (Crustacea, Amphipoda): Understanding the links between physiological and behavioural responses

2013 ◽  
Vol 140-141 ◽  
pp. 106-116 ◽  
Author(s):  
Céline Vellinger ◽  
Eric Gismondi ◽  
Vincent Felten ◽  
Philippe Rousselle ◽  
Kahina Mehennaoui ◽  
...  
Keyword(s):  
Gammarus Pulex ◽  
Combined Effects ◽  
Behavioural Responses

Physiological and behavioural responses of Gammarus pulex exposed to acid stress

2008 ◽  
Vol 147 (2) ◽  
pp. 189-197 ◽  
Author(s):  
V. Felten ◽  
G. Charmantier ◽  
M. Charmantier-Daures ◽  
F. Aujoulat ◽  
J. Garric ◽  
...  
Keyword(s):  
Acid Stress ◽  
Gammarus Pulex ◽  
Behavioural Responses

Microtubule nucleation sequence studied by time-resolved x-ray scattering and electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 766-767
Author(s):  
Eva-Maria Mandelkow ◽  
Eckhard Mandelkow ◽  
Joan Bordas
Keyword(s):  
Electron Microscopy ◽  
Dynamic Equilibrium ◽  
Time Resolved ◽  
X Ray ◽  
Additional Information ◽  
X Ray Scattering ◽  
Low Concentrations ◽  
Microtubule Growth ◽  
Ray Patterns ◽  
Ray Scattering

When a solution of microtubule protein is changed from non-polymerising to polymerising conditions (e.g. by temperature jump or mixing with GTP) there is a series of structural transitions preceding microtubule growth. These have been detected by time-resolved X-ray scattering using synchrotron radiation, and they may be classified into pre-nucleation and nucleation events. X-ray patterns are good indicators for the average behavior of the particles in solution, but they are difficult to interpret unless additional information on their structure is available. We therefore studied the assembly process by electron microscopy under conditions approaching those of the X-ray experiment. There are two difficulties in the EM approach: One is that the particles important for assembly are usually small and not very regular and therefore tend to be overlooked. Secondly EM specimens require low concentrations which favor disassembly of the particles one wants to observe since there is a dynamic equilibrium between polymers and subunits.

The CM120 Biotwin: a high-contrast objective lens, optimum cryo-vacuum and improved EDX performance

1995 ◽  
Vol 53 ◽  
pp. 584-585
Author(s):  
Uwe Lücken ◽  
Michael Felsmann ◽  
Wim M. Busing ◽  
Frank de Jong
Keyword(s):  
Life Science ◽  
Focal Length ◽  
Objective Lens ◽  
High Contrast ◽  
Contrast Imaging ◽  
X Ray ◽  
Forming Mechanism ◽  
Similar Image ◽  
High Contrast Imaging ◽  
Low Concentrations

A new microscope for the study of life science specimen has been developed. Special attention has been given to the problems of unstained samples, cryo-specimens and x-ray analysis at low concentrations.A new objective lens with a Cs of 6.2 mm and a focal length of 5.9 mm for high-contrast imaging has been developed. The contrast of a TWIN lens (f = 2.8 mm, Cs = 2 mm) and the BioTWTN are compared at the level of mean and SD of slow scan CCD images. Figure 1a shows 500 +/- 150 and Fig. 1b only 500 +/- 40 counts/pixel. The contrast-forming mechanism for amplitude contrast is dependent on the wavelength, the objective aperture and the focal length. For similar image conditions (same voltage, same objective aperture) the BioTWIN shows more than double the contrast of the TWIN lens. For phasecontrast specimens (like thin frozen-hydrated films) the contrast at Scherzer focus is approximately proportional to the √ Cs.

Immunogold labeling of CMP-KDO synthetase produced by recombinant E. Coli cells

1987 ◽  
Vol 45 ◽  
pp. 924-925
Author(s):  
F. A. Durum ◽  
R. G. Goldman ◽  
T. J. Bolling ◽  
M. F. Miller
Keyword(s):  
Inclusion Bodies ◽  
Large Scale ◽  
Recombinant Dna ◽  
Test System ◽  
Cell Protein ◽  
Gram Negative Bacteria ◽  
Cytoplasmic Inclusions ◽  
Isolation And Purification ◽  
E Coli ◽  
Low Concentrations

CMP-KDO synthetase (CKS) is an enzyme which plays a key role in the synthesis of LPS, an outer membrane component unique to gram negative bacteria. CKS activates KDO to CMP-KDO for incorporation into LPS. The enzyme is normally present in low concentrations (0.02% of total cell protein) which makes it difficult to perform large scale isolation and purification. Recently, the gene for CKS from E. coli was cloned and various recombinant DNA constructs overproducing CKS several thousandfold (unpublished data) were derived. Interestingly, no cytoplasmic inclusions of overproduced CKS were observed by EM (Fig. 1) which is in contrast to other reports of large proteinaceous inclusion bodies in various overproducing recombinant strains. The present immunocytochemical study was undertaken to localize CKS in these cells.Immune labeling conditions were first optimized using a previously described cell-free test system. Briefly, this involves soaking small blocks of polymerized bovine serum albumin in purified CKS antigen and subjecting them to various fixation, embedding and immunochemical conditions.

Phase behavior of cationic and anionic surfactants at low concentrations

1992 ◽  
Vol 50 (1) ◽  
pp. 694-695
Author(s):  
E. Naranjo
Keyword(s):  
Phase Behavior ◽  
Structural Characterization ◽  
Dynamic Systems ◽  
Anionic Surfactants ◽  
Intermolecular Forces ◽  
Stable Form ◽  
Surfactant Mixtures ◽  
Low Concentrations ◽  
Cationic And Anionic Surfactants ◽  
General Method

Equilibrium vesicles, those which are the stable form of aggregation and form spontaneously on mixing surfactant with water, have never been demonstrated in single component bilayers and only rarely in lipid or surfactant mixtures. Designing a simple and general method for producing spontaneous and stable vesicles depends on a better understanding of the thermodynamics of aggregation, the interplay of intermolecular forces in surfactants, and an efficient way of doing structural characterization in dynamic systems.

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