In vitro inhibitory effects of Polygonum cuspidatum on bacterial viability and virulence factors of Streptococcus mutans and Streptococcus sobrinus

2006 ◽  
Vol 51 (12) ◽  
pp. 1131-1140 ◽  
Author(s):  
Ju-Hee Song ◽  
Shin-Kyu Kim ◽  
Kee-Wan Chang ◽  
Seong-Kyu Han ◽  
Ho-Keun Yi ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Virulence Factors ◽  
Polygonum Cuspidatum ◽  
Streptococcus Sobrinus ◽  
Inhibitory Effects ◽  
Bacterial Viability

scholarly journals Efeito antimicrobiano in vitro do extrato de jabuticaba [Myrciaria cauliflora (Mart.)O.Berg.] sobre Streptococcus da cavidade oral

2009 ◽  
Vol 11 (1) ◽  
pp. 79-83 ◽  
Author(s):  
C.M. Carvalho ◽  
M.R. Macedo-Costa ◽  
M.S.V. Pereira ◽  
J.S. Higino ◽  
L.F.P.C. Carvalho ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Streptococcus Sobrinus ◽  
Streptococcus Sanguis

Este estudo avaliou a ação do extrato etanólico das folhas de Myrciaria cauliflora (Mart.)O.Berg. sobre três culturas de Streptococcus formadoras do biofilme dental. A atividade antimicrobiana foi determinada pelo método de difusão em meio sólido. Os dados obtidos foram satisfatórios para todas as espécies ensaiadas (Streptococcus mutans ATCC 25175, Streptococcus sobrinus ATCC 27609 e Streptococcus sanguis ATCC 10557) gerando halos de inibição quando utilizado o extrato puro e diluições 1:2 e 1:4. Os resultados incentivam a realização de novas pesquisas que viabilizem a produção de compostos com finalidade terapêutica que possam ser utilizados clinicamente na odontologia.

scholarly journals Activity of Human β-Defensin 3 Alone or Combined with Other Antimicrobial Agents against Oral Bacteria

2003 ◽  
Vol 47 (10) ◽  
pp. 3349-3351 ◽  
Author(s):  
Giuseppantonio Maisetta ◽  
Giovanna Batoni ◽  
Semih Esin ◽  
Filippo Luperini ◽  
Manuela Pardini ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Bactericidal Activity ◽  
Lactobacillus Acidophilus ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Bactericidal Effect ◽  
Oral Bacteria ◽  
Actinobacillus Actinomycetemcomitans ◽  
Streptococcus Sobrinus

ABSTRACT The in vitro activities of human β-defensin 3 (hBD-3) alone or combined with lysozyme, metronidazole, amoxicillin, and chlorhexidine were investigated with the oral bacteria Streptococcus mutans, Streptococcus sanguinis, Streptococcus sobrinus, Lactobacillus acidophilus, Actinobacillus actinomycetemcomitans, and Porphyromonas gingivalis. hBD-3 showed bactericidal activity against all of the bacterial species tested. The bactericidal effect was enhanced when the peptide was used in combination with the antimicrobial agents mentioned above.

scholarly journals In Vitro Evaluation of Bacterial Adhesion and Bacterial Viability of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis on the Abutment Surface of Titanium and Zirconium Dental Implants

2019 ◽  
Vol 2019 ◽  
pp. 1-5 ◽  
Author(s):  
Ana Stefany Meza-Siccha ◽  
Miguel Angel Aguilar-Luis ◽  
Wilmer Silva-Caso ◽  
Fernando Mazulis ◽  
Carolina Barragan-Salazar ◽  
...  
Keyword(s):  
Dental Implants ◽  
Streptococcus Mutans ◽  
Porphyromonas Gingivalis ◽  
Bacterial Adhesion ◽  
Bacterial Species ◽  
Bacterial Adherence ◽  
Bacterial Viability ◽  
Streptococcus Sanguinis ◽  
Group 2

Objective. To evaluate the in vitro adherence and viability of 3 bacterial species Streptococcus mutans (ATCC 25175), Streptococcus sanguinis (ATCC 10556), and Porphyromonas gingivalis (ATCC 33277) on the surfaces of dental implants of titanium, zirconium, and their respective fixing screws. Methods. Two analysis groups were formed: group 1 with 3 titanium pillars and group 2 with 3 zirconium pillars, each with their respective fixing screws. Each of these groups was included in tubes with bacterial cultures of Streptococcus mutans (ATCC 25175), Streptococcus sanguinis (ATCC 10556), and Porphyromonas gingivalis (ATCC 33277). These samples were incubated at 37°C under anaerobic conditions. Bacterial adherence was assessed by measurement of the change in colony-forming units (CFU), and bacterial viability was evaluated with the colorimetric test of 3-(4,5-dimethylthiazol-2)-2,5 diphenyl tetrazolium bromide (MTT). Results. The bacterial adhesion in the titanium abutments was higher for Streptococcus mutans (190.90 CFU/mL), and the viability was greater in Porphyromonas gingivalis (73.22%). The zirconium abutment group showed the highest adherence with Streptococcus mutans (331.82 CFU/mL) and the highest bacterial viability with the S. sanguinis strain (38.42%). The titanium fixation screws showed the highest adhesion with S. sanguinis (132.5 CFU/mL) compared to the zirconium fixation screws where S. mutans had the highest adhesion (145.5 CFU/mL). The bacterial viability of S. mutans was greater both in the titanium fixation screws and in the zirconium fixation screws 78.04% and 57.38%, respectively. Conclusions. Our results indicate that there is in vitro bacterial adherence and viability in both titanium abutments and zirconium abutments and fixation screws for both. Streptococcus mutans is the microorganism that shows the greatest adherence to the surfaces of both titanium and zirconium and the fixing screws of the latter. On the contrary, bacterial viability is greater on the titanium abutments with P. gingivalis than on the zirconium abutments with S. sanguinis. With respect to the fixation screws, in both cases, the viability of S. mutans was greater with respect to the other bacteria. In general, the titanium abutments showed less adherence but greater bacterial viability.

Inhibitory Effects of Hybrid Liposomes Including Extracts from Polygonum cuspidatum on the Growth of Leukemia Cells in Vitro

2011 ◽  
Vol 37 (2) ◽  
pp. 192-196
Author(s):  
Hideaki Ichihara ◽  
Kazuki Tatsumi ◽  
Koichi Goto ◽  
Yoko Matsumoto ◽  
Ryuichi Ueoka
Keyword(s):  
Leukemia Cells ◽  
Polygonum Cuspidatum ◽  
Inhibitory Effects

In Vitro Studies of Xylitol and Erythritol Inhibition of Streptococcus Mutans and Streptococcus Sobrinus Growth and Biofilm Production

2020 ◽  
Vol 44 (5) ◽  
pp. 307-314
Author(s):  
M l Cannon ◽  
M Merchant ◽  
W Kabat ◽  
Le Catherine ◽  
K White ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Culture Collection ◽  
Streptococcus Sobrinus ◽  
Biofilm Growth ◽  
Biomass Formation ◽  
Bactericidal Effects ◽  
Dose Dependent Fashion ◽  
Polyol Influence ◽  
Microtiter Assay

The aim of this study was to evaluate synergy and inhibitory effects of xylitol and erythritol on Streptococcus mutans and Streptococcus sobrinus growth and biomass production on a polystyrene plastic surface. Study design; S. mutans and sobrinus strains (American Type Culture Collection reference strains 31341, 35668, 25175, sobrinus 33478) were cultivated in media (Todd Hewitt Broth with 1% sucrose or heart-brain infusion broth with 1% sucrose) at differing concentrations of xylitol or erythritol in microtiter assay plates incubated for 48 hours. Bacterial growth was quantified and measured by optical density using a microplate reader. Experiments assessing synergy and biofilm growth were carried out also using microdilution assays. All four strains were inhibited by 30% (w/v) xylitol, and 15% erythritol at 150mg/ml erythritol, 2/4 strains had reduced growth; at 270mg/ml, 4/4 strains were inhibited. Bactericidal effects were not observed at any polyol concentration. Combinations of both polyols in a checker board array were used to determine if there were any benefits of polyol combinations. Results The combination studies yielded mixed outcomes with indifference in growth for strains 68 and 78, potential additive effect for strain 75 and possible antagonism for strain 41. Assessment of biomass formation and polyol interference were also performed post MIC assessment. Strains 41, 68 and 75 produced significant biomass in the absence of either polyol. Both polyols inhibited biomass formation in a dose-dependent fashion. Strain 75 is a poor biomass producer and could not be assessed for polyol effects in our assay. Conclusion: Our results demonstrate significant polyol influence on the oral Streptococcal strains tested in our laboratory.

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