Contrasting genetic diversity and intra-population polymorphism of the invasive pest Henosepilachna vigintioctopunctata (Coleoptera, Coccinellidae): A DNA barcoding approach

DNA barcoding is well-known to support morphological species identification and it can be helpful for unveiling unexpected populations divergence patterns, especially in the context of the impacts on species posed by global change. In this note, we provided the first Italian record of the alpine mining bee Andrena allosa Warncke, 1975, confirmed with DNA barcoding. In addition, genetic identification of a specimen of Andrena praecox (Scopoli 1753) from western Italy pointed to an unexpected intraspecific genetic structuring at COI DNA barcoding region, with sequences from the Italian and the western sector of its global distribution differing 2.22% (p-dist) from populations of the eastern sector. Given the relevance of these records and of the genetic identity of bee populations from Italy, we argue that implementing molecular surveys in bee monitoring would surely contribute to the conservation of these important pollinators.

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Genetic Diversity of Pediculus humanus capitis (Phthiraptera: Pediculidae) in Peninsular Malaysia and Molecular Detection of Its Potential Associated Pathogens

Journal of Medical Entomology ◽

10.1093/jme/tjz234 ◽

2019 ◽

Vol 57 (3) ◽

pp. 915-926

Author(s):

Aida Syafinaz Mokhtar ◽

Yee Ling Lau ◽

John-James Wilson ◽

Noraishah Mydin Abdul-Aziz

Keyword(s):

Genetic Diversity ◽

Dna Barcoding ◽

Pathogenic Bacteria ◽

Peninsular Malaysia ◽

Molecular Evidence ◽

Head Lice ◽

Pediculus Humanus Capitis ◽

Accurate Identification ◽

Pediculosis Capitis ◽

Pediculus Humanus

Abstract Pediculosis capitis caused by Pediculus humanus capitis (De Geer) is endemic all over the world, and children are mostly affected, particularly those living in overcrowded institutions. Several studies have shown that P. h. capitis carried human pathogenic bacteria, suggesting the potential role of head lice in the transmission of pathogens to humans. In this study, we determined the genetic diversity of head lice collected from welfare homes sheltering underprivileged children by using DNA barcoding and demonstrated the presence of Acinetobacter spp., Serratia marcescens, and Staphylococcus aureus in head lice, which have never been investigated before in Malaysia. Cox1 DNA barcoding identified the head lice, P. h. capitis collected from welfare homes across two geographical areas of Peninsular Malaysia as belonging to clades A, B, and D. Acinetobacter bacteria: Acinetobacter guillouiae, Acinetobacter junii, Acinetobacter baumannii, and Acinetobacter nosocomialis were detected in head lice belonging to clades A and also D. In addition, DNA from S. marcescens and S. aureus were also detected in both clades A and D. To our knowledge, this is the first report on the genetic diversity of head lice in Malaysia through DNA barcoding, as well as the first to provide molecular evidence on the type of bacteria occurring in head lice in Malaysia. It is anticipated that the DNA barcoding technique used in this study will be able to provide rapid and accurate identification of arthropods, in particular, medically important ectoparasites.

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Molecular Identification and Genetic Diversity of Thalassia hemprichii Through DNA Barcoding Using Internal Transcribed Spacer gene (ITS) from Awur Bay Jepara, Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/255/1/012037 ◽

2019 ◽

Vol 255 ◽

pp. 012037

Author(s):

A N Faozi ◽

T Harisam ◽

M Pharmawati ◽

B Marhaeni

Keyword(s):

Genetic Diversity ◽

Dna Barcoding ◽

Molecular Identification ◽

Internal Transcribed Spacer ◽

Thalassia Hemprichii

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Genetic diversity of genus Vespa including an invaded species of V. velutina (Hymenoptera: Vespidae) in Korea inferred from DNA barcoding data

Journal of Asia-Pacific Entomology ◽

10.1016/j.aspen.2020.04.004 ◽

2020 ◽

Vol 23 (2) ◽

pp. 540-545

Author(s):

Saeed Mohamadzade Namin ◽

Chuleui Jung

Keyword(s):

Genetic Diversity ◽

Dna Barcoding

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Mitochondrial DNA revealed the extent of genetic diversity and invasion origin of populations from two separate invaded areas of a newly invasive pest,Cydia pomonella(L.) (Lepidoptera: Tortricidae) in China

Bulletin of Entomological Research ◽

10.1017/s0007485315000334 ◽

2015 ◽

Vol 105 (4) ◽

pp. 485-496 ◽

Cited By ~ 7

Author(s):

Y. Li ◽

X. Duan ◽

X. Qiao ◽

X. Li ◽

K. Wang ◽

...

Keyword(s):

Genetic Diversity ◽

Continuous Monitoring ◽

Insect Pest ◽

Cydia Pomonella ◽

Haplotype Diversity ◽

Northwestern China ◽

Invasive Pest ◽

Mitochondrial Coi ◽

Similar Haplotype ◽

Northeast Of China

AbstractCydia pomonellais a serious invasive insect pest in China, and has caused severe damage to the production of apple and pear in its invaded areas. This species is distributing in the northwest and northeast of China, but no occurrence of it has been recorded in the large areas (about 3000–5000 km away) between the invaded northwestern and northeastern regions despite continuous monitoring. As yet the genetic diversity and invasion origin of theC. pomonellapopulations in Northwestern and Northeastern China is obscure. In this study, we investigate the genetic diversity of 14 populations ofC. pomonellasampled throughout the main distribution regions in Northwestern (Xinjiang and Gansu Provinces) and Northeastern (Heilongjiang Province) China and compared them with nine populations from Europe and other continents using the mitochondrial COI, COII and Cytb genes. Both the populations from Northeastern and Northwestern China shared some haplotypes with populations from other countries. Haplotypes of the three mitochondrial genes had a different distribution in Northeastern and Northwestern China. The northeastern populations had more private haplotypes than the northwestern populations. A large number of the individuals from northwestern populations shared a few haplotypes of each of the three genes. The haplotype numbers and haplotype diversities of the northeastern populations were similar to those of field populations in other countries, but were higher than those of the northwestern populations. Populations from the Northwestern China showed similar haplotype number and haplotype diversity. We conclude that the population genetic background ofC. pomonellapopulations in Northeastern and Northwestern China varies due to different invasion sources and that this should be considered before the application of new pest control tactics.

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DNA barcoding of odonates from the Upper Plata basin: Database creation and genetic diversity estimation

PLoS ONE ◽

10.1371/journal.pone.0182283 ◽

2017 ◽

Vol 12 (8) ◽

pp. e0182283 ◽

Cited By ~ 8

Author(s):

Ricardo Koroiva ◽

Mateus Pepinelli ◽

Marciel Elio Rodrigues ◽

Fabio de Oliveira Roque ◽

Aline Pedroso Lorenz-Lemke ◽

...

Keyword(s):

Genetic Diversity ◽

Dna Barcoding

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Population Polymorphism of Nuclear Mitochondrial DNA Insertions Reveals Widespread Diploidy Associated with Loss of Heterozygosity in Debaryomyces hansenii

Eukaryotic Cell ◽

10.1128/ec.00263-09 ◽

2010 ◽

Vol 9 (3) ◽

pp. 449-459 ◽

Cited By ~ 20

Author(s):

Noémie Jacques ◽

Christine Sacerdot ◽

Meriem Derkaoui ◽

Bernard Dujon ◽

Odile Ozier-Kalogeropoulos ◽

...

Keyword(s):

Genetic Diversity ◽

Flow Cytometry ◽

Mitochondrial Dna ◽

Loss Of Heterozygosity ◽

Debaryomyces Hansenii ◽

Intraspecific Variability ◽

Content Type ◽

Population Polymorphism ◽

First Time

ABSTRACT Debaryomyces hansenii, a yeast that participates in the elaboration of foodstuff, displays important genetic diversity. Our recent phylogenetic classification of this species led to the subdivision of the species into three distinct clades. D. hansenii harbors the highest number of nuclear mitochondrial DNA (NUMT) insertions known so far for hemiascomycetous yeasts. Here we assessed the intraspecific variability of the NUMTs in this species by testing their presence/absence first in 28 strains, with 21 loci previously detected in the completely sequenced strain CBS 767T, and second in a larger panel of 77 strains, with 8 most informative loci. We were able for the first time to structure populations in D. hansenii, although we observed little NUMT insertion variability within the clades. We determined the chronology of the NUMT insertions, which turned out to correlate with the previously defined taxonomy and provided additional evidence that colonization of nuclear genomes by mitochondrial DNA is a dynamic process in yeast. In combination with flow cytometry experiments, the NUMT analysis revealed the existence of both haploid and diploid strains, the latter being heterozygous and resulting from at least four crosses among strains from the various clades. As in the diploid pathogen Candida albicans, to which D. hansenii is phylogenetically related, we observed a differential loss of heterozygosity in the diploid strains, which can explain some of the large genetic diversity found in D. hansenii over the years.

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