Psychosocial stress-related changes in gene expression of norepinephrine biosynthetic enzymes in stellate ganglia of adult rats

ABSTRACT Chronic isolation of adult animals represents a form of psychological stress that produces sympatho-adrenomedullar activation. Exercise training acts as an important modulator of sympatho-adrenomedullary system. This study aimed to investigate physical exercise-related changes in gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase, dopamine-ß-hydroxylase and phenylethanolamine N-methyltransferase) and cyclic adenosine monophosphate response element-binding (CREB) in the adrenal medulla, concentrations of catecholamines and corticosterone (CORT) in the plasma and the weight of adrenal glands of chronically psychosocially stressed adult rats exposed daily to 20 min treadmill running for 12 weeks. Also, we examined how additional acute immobilization stress changes the mentioned parameters. Treadmill running did not result in modulation of gene expression of catecholamine synthesizing enzymes and it decreased the level of CREB mRNA in the adrenal medulla of chronically psychosocially stressed adult rats. The potentially negative physiological adaptations after treadmill running were recorded as increased concentrations of catecholamines and decreased morning CORT concentration in the plasma, as well as the adrenal gland hypertrophy of chronically psychosocially stressed rats. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. Treadmill exercise does not change the activity of sympatho-adrenomedullary system of chronically psychosocially stressed rats.

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Behavioral sensitization induced by methamphetamine causes differential alterations in gene expression and histone acetylation of the prefrontal cortex in rats

BMC Neuroscience ◽

10.1186/s12868-021-00616-5 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Hui Li ◽

Jing-An Chen ◽

Qian-Zhi Ding ◽

Guan-Yi Lu ◽

Ning Wu ◽

...

Keyword(s):

Gene Expression ◽

Prefrontal Cortex ◽

Histone Acetylation ◽

Differentially Expressed Genes ◽

Behavioral Sensitization ◽

Functional Enrichment ◽

Differentially Expressed ◽

Adult Rats ◽

Mrna Microarray ◽

H3 Acetylation

Abstract Background Methamphetamine (METH) is one of the most widely abused illicit substances worldwide; unfortunately, its addiction mechanism remains unclear. Based on accumulating evidence, changes in gene expression and chromatin modifications might be related to the persistent effects of METH on the brain. In the present study, we took advantage of METH-induced behavioral sensitization as an animal model that reflects some aspects of drug addiction and examined the changes in gene expression and histone acetylation in the prefrontal cortex (PFC) of adult rats. Methods We conducted mRNA microarray and chromatin immunoprecipitation (ChIP) coupled to DNA microarray (ChIP-chip) analyses to screen and identify changes in transcript levels and histone acetylation patterns. Functional enrichment analyses, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, were performed to analyze the differentially expressed genes. We then further identified alterations in ANP32A (acidic leucine-rich nuclear phosphoprotein-32A) and POU3F2 (POU domain, class 3, transcription factor 2) using qPCR and ChIP-PCR assays. Results In the rat model of METH-induced behavioral sensitization, METH challenge caused 275 differentially expressed genes and a number of hyperacetylated genes (821 genes with H3 acetylation and 10 genes with H4 acetylation). Based on mRNA microarray and GO and KEGG enrichment analyses, 24 genes may be involved in METH-induced behavioral sensitization, and 7 genes were confirmed using qPCR. We further examined the alterations in the levels of the ANP32A and POU3F2 transcripts and histone acetylation at different periods of METH-induced behavioral sensitization. H4 hyperacetylation contributed to the increased levels of ANP32A mRNA and H3/H4 hyperacetylation contributed to the increased levels of POU3F2 mRNA induced by METH challenge-induced behavioral sensitization, but not by acute METH exposure. Conclusions The present results revealed alterations in transcription and histone acetylation in the rat PFC by METH exposure and provided evidence that modifications of histone acetylation contributed to the alterations in gene expression caused by METH-induced behavioral sensitization.

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Gene expression of catecholamine biosynthetic enzymes following exercise: modulation by age

Neuroscience ◽

10.1016/s0306-4522(01)00020-3 ◽

2001 ◽

Vol 103 (3) ◽

pp. 703-711 ◽

Cited By ~ 32

Author(s):

N. Tümer ◽

H.A. Demirel ◽

L. Serova ◽

E.L. Sabban ◽

C.S. Broxson ◽

...

Keyword(s):

Gene Expression ◽

Biosynthetic Enzymes

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Integrated microRNA and mRNA Gene Expression in Peripheral Blood Mononuclear Cells in Response to Acute Psychosocial Stress: A Repeated-Measures Within-Subject Pilot Study

10.21203/rs.3.rs-322294/v1 ◽

2021 ◽

Author(s):

Magdalena Maria Jurkiewicz ◽

Anett Müller-Alcazar ◽

Dirk Moser ◽

Indralatha Jayatilaka ◽

Anatoly Mikhailik ◽

...

Keyword(s):

Gene Expression ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Psychosocial Stress ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Time Points ◽

Blood Mononuclear Cells ◽

The Impact ◽

Acute Psychosocial Stress

Abstract Objective: The impact of psychosocial stress on a variety of negative health outcomes is well documented, with current research efforts directed at possible mechanisms. Here, we focused on a potential mechanism involving differential expression of mRNA and microRNA in response to acute psychosocial stress. We utilized a validated behavioral paradigm, the Trier Social Stress Test (TSST), to induce acute psychosocial stress in a cohort of volunteers. Stress reactivity was assessed repeatedly during the TSST using saliva samples that were analyzed for levels of cortisol. Peripheral blood mononuclear cells were extracted from blood drawn at baseline and at two time points following the stress paradigm. Total RNA was extracted, and mRNA and microRNA microarrays were utilized to assess within-subject changes in gene expression between baseline and the two post-stressor time points. Results: For microarray gene expression analysis, we focused on 12 participants who showed a robust cortisol response to the task, as an indicator of robust HPA-axis activation. We discovered a set of mRNAs and miRNAs that exhibited dynamic expression change in response to the TSST in peripheral blood mononuclear cells, further characterizing the link between psychosocial stress and cellular response mechanisms.

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Polyamines and Gene Expression of Biosynthetic Enzymes in Sexual Plant Reproduction

Fertilization in Higher Plants ◽

10.1007/978-3-642-59969-9_1 ◽

1999 ◽

pp. 1-11 ◽

Cited By ~ 2

Author(s):

N. Bagni ◽

A. Tassoni ◽

M. Franceschetti

Keyword(s):

Gene Expression ◽

Plant Reproduction ◽

Biosynthetic Enzymes

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Increased L-CPT-1 activity and altered gene expression in pancreatic islets of malnourished adult rats: a possible relationship between elevated free fatty acid levels and impaired insulin secretion

The Journal of Nutritional Biochemistry ◽

10.1016/j.jnutbio.2007.01.005 ◽

2008 ◽

Vol 19 (2) ◽

pp. 85-90 ◽

Cited By ~ 7

Author(s):

Marise Auxiliadora de Barros Reis ◽

Vanessa Cristina Arantes ◽

Daniel Andrade Cunha ◽

Márcia Queiroz Latorraca ◽

Marcos Hikari Toyama ◽

...

Keyword(s):

Gene Expression ◽

Insulin Secretion ◽

Fatty Acid ◽

Free Fatty Acid ◽

Pancreatic Islets ◽

Adult Rats ◽

Altered Gene Expression ◽

Impaired Insulin Secretion ◽

Impaired Insulin ◽

Altered Gene

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Placental imprinted gene expression mediates the effects of maternal psychosocial stress during pregnancy on fetal growth

Journal of Developmental Origins of Health and Disease ◽

10.1017/s2040174418000545 ◽

2019 ◽

Vol 10 (02) ◽

pp. 196-205

Author(s):

L. Lambertini ◽

Q. Li ◽

Y. Ma ◽

W. Zhang ◽

K. Hao ◽

...

Keyword(s):

Gene Expression ◽

Fetal Growth ◽

Psychosocial Stress ◽

Imprinted Genes ◽

Economic Determinants ◽

Imprinted Gene ◽

Infant Gender ◽

Maternal Resources ◽

Gestational Age At Birth ◽

Lower Birthweight

AbstractImprinted genes uniquely drive and support fetoplacental growth by controlling the allocation of maternal resources to the fetus and affecting the newborn’s growth. We previously showed that alterations of the placental imprinted gene expression are associated with suboptimal perinatal growth and respond to environmental stimuli including socio-economic determinants. At the same time, maternal psychosocial stress during pregnancy (MPSP) has been shown to affect fetal growth. Here, we set out to test the hypothesis that placental imprinted gene expression mediates the effects of MPSP on fetal growth in a well-characterized birth cohort, the Stress in Pregnancy (SIP) Study. We observed that mothers experiencing high MPSP deliver infants with lower birthweight (P=0.047). Among the 109 imprinted genes tested, we detected panels of placental imprinted gene expression of 23 imprinted genes associated with MPSP and 26 with birthweight. Among these genes, five imprinted genes (CPXM2, glucosidase alpha acid (GAA), GPR1, SH3 and multiple ankyrin repeat domains 2 (SHANK2) and THSD7A) were common to the two panels. In multivariate analyses, controlling for maternal age and education and gestational age at birth and infant gender, two genes, GAA and SHANK2, each showed a 22% mediation of MPSP on fetal growth. These data provide new insights into the role that imprinted genes play in translating the maternal stress message into a fetoplacental growth pattern.

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