Roles of YB-1 under arsenite-induced stress: Translational activation of HSP70 mRNA and control of the number of stress granules

2014 ◽  
Vol 1840 (3) ◽  
pp. 985-992 ◽  
Author(s):  
Toru Tanaka ◽  
Sachiyo Ohashi ◽  
Shunsuke Kobayashi
Keyword(s):  
Stress Granules ◽  
Hsp70 Mrna ◽  
Induced Stress ◽  
Translational Activation ◽  
And Control

scholarly journals An Integrated biomarker approach for explaining the potency of exogenous glucose on transportation induced stress in Labeo rohita fingerlings

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Abhilipsa Biswal ◽  
Prem Prakash Srivastava ◽  
Gopal Krishna ◽  
Tapas Paul ◽  
Prasenjit Pal ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Optimum Dose ◽  
Control Group ◽  
Stress Hormone ◽  
Hsp70 Mrna ◽  
Exogenous Glucose ◽  
Induced Stress ◽  
Biomarker Response ◽  
Stress Relieving ◽  
And Control

AbstractTransportation of fish seed is a complex phenomenon associated with multiple kinds of stressors that simultaneously affect the fish in a confined environment, causing stress and mortality. The present study investigated the stress-relieving effect of exogenous glucose as a water additive in different concentrations (0.1, 0.2, 0.3, and 0.4%) during simulated transportation (12 h) of L. rohita fingerlings. The integrated biomarker response (IBR) index is a holistic tool to determine the optimum dose of exogenous glucose for mitigating transportation stress in fish. Based on selected biomarkers related to the stress hormone, serum biochemistry, oxidative stress, and HSP70 mRNA expression, the IBR index is calculated for each treatment and control group. The result showed a significant change in the level of stress hormone cortisol, enzymes (SGPT, LDH, MDH, SOD, CAT) and metabolites (serum glucose, triglyceride, creatinine) along with an upregulation in liver HSP70 mRNA expression. IBR index suggests that 0.2% glucose exhibited the lowest multi-biomarker stress response in comparison to other treatments and control. Therefore, the use of 0.2% glucose as a water additive will provide a solution to transportation induced stress in L. rohita fingerling and will underwrite the success of grow-out fish culture in days to come.

scholarly journals Dynamic m6A methylation facilitates mRNA triaging to stress granules

2018 ◽  
Vol 1 (4) ◽  
pp. e201800113 ◽  
Author(s):  
Maximilian Anders ◽  
Irina Chelysheva ◽  
Ingrid Goebel ◽  
Timo Trenkner ◽  
Jun Zhou ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Messenger Rna ◽  
Stress Granules ◽  
Rna Metabolism ◽  
Methylation Pattern ◽  
Domain Family ◽  
Induced Stress ◽  
And Function ◽  
Selective Mechanism

Reversible post-transcriptional modifications on messenger RNA emerge as prevalent phenomena in RNA metabolism. The most abundant among them is N6-methyladenosine (m6A) which is pivotal for RNA metabolism and function; its role in stress response remains elusive. We have discovered that in response to oxidative stress, transcripts are additionally m6A modified in their 5′ vicinity. Distinct from that of the translationally active mRNAs, this methylation pattern provides a selective mechanism for triaging mRNAs from the translatable pool to stress-induced stress granules. These stress-induced newly methylated sites are selectively recognized by the YTH domain family 3 (YTHDF3) “reader” protein, thereby revealing a new role for YTHDF3 in shaping the selectivity of stress response. Our findings describe a previously unappreciated function for RNA m6A modification in oxidative-stress response and expand the breadth of physiological roles of m6A.

scholarly journals Arabidopsis CALMODULIN-LIKE 38 Regulates Hypoxia-Induced Autophagy of SUPPRESSOR OF GENE SILENCING 3 Bodies

2021 ◽  
Vol 12 ◽  
Author(s):  
Sterling Field ◽  
William Craig Conner ◽  
Daniel M. Roberts
Keyword(s):  
Gene Silencing ◽  
Mrna Translation ◽  
Stress Granules ◽  
Stress Granule ◽  
Calcium Sensor ◽  
In Planta ◽  
Hypoxia Stress ◽  
Induced Stress ◽  
Submergence Stress ◽  
Sensor Protein

During the energy crisis associated with submergence stress, plants restrict mRNA translation and rapidly accumulate stress granules that act as storage hubs for arrested mRNA complexes. One of the proteins associated with hypoxia-induced stress granules in Arabidopsis thaliana is the calcium-sensor protein CALMODULIN-LIKE 38 (CML38). Here, we show that SUPPRESSOR OF GENE SILENCING 3 (SGS3) is a CML38-binding protein, and that SGS3 and CML38 co-localize within hypoxia-induced RNA stress granule-like structures. Hypoxia-induced SGS3 granules are subject to turnover by autophagy, and this requires both CML38 as well as the AAA+-ATPase CELL DIVISION CYCLE 48A (CDC48A). CML38 also interacts directly with CDC48A, and CML38 recruits CDC48A to CML38 granules in planta. Together, this work demonstrates that SGS3 associates with stress granule-like structures during hypoxia stress that are subject to degradation by CML38 and CDC48-dependent autophagy. Further, the work identifies direct regulatory targets for the hypoxia calcium-sensor CML38, and suggest that CML38 association with stress granules and associated regulation of autophagy may be part of the RNA regulatory program during hypoxia stress.

scholarly journals Dynamic m6A methylation facilitates mRNA triaging to stress granules

2018 ◽  
Author(s):  
Maximilian Anders ◽  
Irina Chelysheva ◽  
Ingrid Goebel ◽  
Timo Trenkner ◽  
Jun Zhou ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Messenger Rna ◽  
Stress Granules ◽  
Rna Metabolism ◽  
Methylation Pattern ◽  
Domain Family ◽  
Induced Stress ◽  
And Function ◽  
Selective Mechanism

Summary blurbm6A-modification in the 5’ vicinity of the coding sequence of transcripts provides a selective mechanism for triaging mRNAs to stress granules and is mediated by YTHDF3 ‘reader’ protein.AbstractReversible post-transcriptional modifications on messenger RNA emerge as prevalent phenomena in RNA metabolism. The most abundant among them is N6-methyladenosine (m6A) which is pivotal for RNA metabolism and function, its role in stress response remains elusive. We have discovered that in response to oxidative stress, transcripts are additionally m6A-modified in their 5’ vicinity. Distinct from that of the translationally-active mRNAs, this methylation pattern provides a selective mechanism for triaging mRNAs from the translatable pool to stress-induced stress granules. These stress-induced newly methylated sites are selectively recognized by the YTH domain family 3 (YTHDF3) ‘reader’ protein, thereby revealing a new role for YTHDF3 in shaping the selectivity of stress response. Our findings describe a previously unappreciated function for RNA m6A modification in the oxidative-stress response and expand the breadth of physiological roles of m6A.

scholarly journals ZFAND1 Recruits p97 and the 26S Proteasome to Promote the Clearance of Arsenite-Induced Stress Granules

2018 ◽  
Vol 70 (5) ◽  
pp. 906-919.e7 ◽  
Author(s):  
Ankit Turakhiya ◽  
Susanne R. Meyer ◽  
Gabriella Marincola ◽  
Stefanie Böhm ◽  
Jens T. Vanselow ◽  
...  
Keyword(s):  
Stress Granules ◽  
26S Proteasome ◽  
Induced Stress

Stress-Induced Gastric Lesions in Mice

1965 ◽  
Vol 16 (3) ◽  
pp. 941-946 ◽  
Author(s):  
John D. Frisone ◽  
Walter B. Essman
Keyword(s):  
Food Deprivation ◽  
Gastric Lesion ◽  
Gastric Tissue ◽  
Gastric Lesions ◽  
Gastric Pathology ◽  
Induced Stress ◽  
Food Retention ◽  
And Control

Groups of light (13-21 gm.) and heavier (22-30 gm.) mice were subdivided and either immobilized for 48 hr. (Experimental) or just food deprived for the same period (Control). The stomachs were removed, food contents noted, and the gastric tissue was examined for lesions and rated for the severity of pathology Experimental and control Ss differed significantly only with respect to the amount of food contained in the stomach and not with respect to the rated severity of the gastric lesion; a decrease in the severity of gastric pathology and a lowered incidence of gastric pathology emerged as a function of increased weight and food retention. It was apparent that immobilization-induced stress contributed little more to the incidence and severity of gastric lesions in mice than did food deprivation alone.

scholarly journals The Effects of Some Pesticides on Spore Germination and Gametophyte Differentiation in Athyrium filix-femina (L.) Roth. and Polypodium vulgare L.

2013 ◽  
Vol 41 (2) ◽  
pp. 458 ◽  
Author(s):  
Liliana Cristina SOARE ◽  
CodruÅ£a-Mihaela DOBRESCU ◽  
Monica POPESCU ◽  
Alina Gabriela BOERU
Keyword(s):  
Negative Correlation ◽  
Spore Germination ◽  
Metallic Copper ◽  
Significant Negative Correlation ◽  
Germination Percentage ◽  
Copper Hydroxide ◽  
Testing Period ◽  
Induced Stress ◽  
Fern Species ◽  
And Control

The aim of this study was to assess the effects of a fungicide (copper hydroxide with 50% metallic copper) (Co) and of an insecticide (bifenthrin 100 g/l) (B) on spore germination and gametophyte development in the fern species Athyrium filix-femina (L.) Schott. and Polypodium vulgare L. The experimental variants were: V1Co: 0.1 gr fungicide/100 ml Knop solution, V2Co: 0.2 gr fungicide/100 ml Knop solution, V3Co: 3 gr fungicide/100 ml Knop solution, V1B: 0.01 ml insecticide/100 ml Knop solution, V2B: 0.02 ml insecticide/100 ml Knop solution, V3B: 0.04 ml insecticide/100 ml Knop solution and Control (C): 100 ml Knop solution. Co inhibited spore germination in all the experimental variants tested on the species Athyrium filix-femina. In the V3Co variant, after 24 days, no spore germinated. B also inhibited spore germination in all the experimental variants. In Polypodium vulgare, Co significantly inhibited spore germination. In the experimental variants containing B, only in the V3B variant the germination is significantly inhibited. Calculations showed a significant negative correlation between the germination percentage and the concentration of pesticides. The fungicide also affected gametophyte differentiation, which happened much more slowly in both species. The rhizoids of the gametophytes of Polypodium vulgare showed modifications in their differentiation and morphology that could also be related to alterations in their biochemical composition. The experimental variants with the highest concentration of insecticide resulted in the differentiation of abnormal gametophytes growing in a tridimensional cellular mass with callus morphology. The responses of plants to the induced stress produced during the testing period may be used as biomarkers of environmental pollution caused by pesticides.

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