Identification of essential intron sequences that enhance gene expression independently of splicing in the yeast Saccharomyces cerevisiae

2022 ◽  
pp. 194784
Author(s):  
Hiroki Kikuta ◽  
Satoshi Goto ◽  
Masaki Kondo ◽  
Rinji Akada ◽  
Hisashi Hoshida
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Yeast Saccharomyces Cerevisiae ◽  
Enhance Gene Expression

Positive control of sporulation-specific genes by the IME1 and IME2 products in Saccharomyces cerevisiae

1990 ◽  
Vol 10 (5) ◽  
pp. 2104-2110
Author(s):  
A P Mitchell ◽  
S E Driscoll ◽  
H E Smith
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Positive Control ◽  
Spore Formation ◽  
Specific Gene ◽  
Heterologous Promoter ◽  
Yeast Saccharomyces Cerevisiae ◽  
Specific Gene Expression ◽  
Rapid Induction

In the yeast Saccharomyces cerevisiae, meiosis and spore formation require the induction of sporulation-specific genes. Two genes are thought to activate the sporulation program: IME1 and IME2 (inducer of meiosis). Both genes are induced upon entry into meiosis, and IME1 is required for IME2 expression. We report here that IME1 is essential for expression of four sporulation-specific genes. In contrast, IME2 is not absolutely essential for expression of the sporulation-specific genes, but contributes to their rapid induction. Expression of IME2 from a heterologous promoter permits the expression of these sporulation-specific genes, meiotic recombination, and spore formation in the absence of IME1. We propose that the IME1 and IME2 products can each activate sporulation-specific genes independently. In addition, the IME1 product stimulates sporulation-specific gene expression indirectly through activation of IME2 expression.

scholarly journals Role of IME1 expression in regulation of meiosis in Saccharomyces cerevisiae.

1990 ◽  
Vol 10 (12) ◽  
pp. 6103-6113 ◽  
Author(s):  
H E Smith ◽  
S S Su ◽  
L Neigeborn ◽  
S E Driscoll ◽  
A P Mitchell
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Specific Gene ◽  
Alpha Cells ◽  
Cell Type ◽  
Yeast Saccharomyces Cerevisiae ◽  
Specific Gene Expression ◽  
Gal1 Promoter ◽  
Acid Protein

Two signals are required for meiosis and spore formation in the yeast Saccharomyces cerevisiae: starvation and the MAT products a1 and alpha 2, which determine the a/alpha cell type. These signals lead to increased expression of the IME1 (inducer of meiosis) gene, which is required for sporulation and sporulation-specific gene expression. We report here the sequence of the IME1 gene and the consequences of IME1 expression from the GAL1 promoter. The deduced IME1 product is a 360-amino-acid protein with a tyrosine-rich C-terminal region. Expression of PGAL1-IME1 in vegetative a/alpha cells led to moderate accumulation of four early sporulation-specific transcripts (IME2, SPO11, SPO13, and HOP1); the transcripts accumulated 3- to 10-fold more after starvation. Two sporulation-specific transcripts normally expressed later (SPS1 and SPS2) did not accumulate until PGAL1-IME1 strains were starved, and the intact IME1 gene was not activated by PGAL1-IME1 expression. In a or alpha cells, which lack alpha 2 or a1, expression of PGAL1-IME1 led to the same pattern of IME2 and SPO13 expression as in a/alpha cells, as measured with ime2::lacZ and spo13::lacZ fusions. Thus, in wild-type strains, the increased expression of IME1 in starved a/alpha cells can account entirely for cell type control, but only partially for nutritional control, of early sporulation-specific gene expression. PGAL1-IME1 expression did not cause growing cells to sporulate but permitted efficient sporulation of amino acid-limited cells, which otherwise sporulated poorly. We suggest that IME1 acts primarily as a positive regulator of early sporulation-specific genes and that growth arrest is an independent prerequisite for execution of the sporulation program.

Insights into Responses to Extreme Environmental Conditions in Humans from Studies on Saccharomyces cerevisiae

2015 ◽  
Vol 65 (6) ◽  
pp. 444
Author(s):  
Ramesh C. Meena ◽  
Amitabha Chakrabarti
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Stress Response ◽  
Gene Expression Data ◽  
Molecular Mechanisms ◽  
Multiple Stressors ◽  
Expression Data ◽  
Environmental Stress Response ◽  
Yeast Saccharomyces Cerevisiae ◽  
Pathways Analysis

<p>The versatility of the yeast experimental model has aided in innumerable ways in the understanding of fundamental cellular functions and has also contributed towards the elucidation of molecular mechanisms underlying several pathological conditions in humans. Genome-wide expression, functional, localization and interaction studies on the yeast Saccharomyces cerevisiae exposed to various stressors have made profound contributions towards the understanding of stress response pathways. Analysis of gene expression data from S. cerevisiae cells indicate that the expression of a common set of genes is altered upon exposure to all the stress conditions examined. This common response to multiple stressors is known as the Environmental stress response. Knowledge gained from studies on the yeast model has now become helpful in understanding stress response pathways and associated disease conditions in humans. Cross-species microarray experiments and analysis of data with ever improving computational methods has led to a better comparison of gene expression data between diverse organisms that include yeast and humans.</p>

scholarly journals Changes in gene expression in the Ras/adenylate cyclase system of Saccharomyces cerevisiae: correlation with cAMP levels and growth arrest.

1993 ◽  
Vol 4 (7) ◽  
pp. 757-765 ◽  
Author(s):  
M Russell ◽  
J Bradshaw-Rouse ◽  
D Markwardt ◽  
W Heideman
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Adenylate Cyclase ◽  
Oxidative Metabolism ◽  
Growth Arrest ◽  
Intracellular Camp ◽  
Adenylate Cyclase System ◽  
Diauxic Shift ◽  
Yeast Saccharomyces Cerevisiae ◽  
Camp Levels

Levels of cyclic 3',5'-cyclic monophosphate (cAMP) play an important role in the decision to enter the mitotic cycle in the yeast, Saccharomyces cerevisiae. In addition to growth arrest at stationary phase, S. cerevisiae transiently arrest growth as they shift from fermentative to oxidative metabolism (the diauxic shift). Experiments examining the role of cAMP in growth arrest at the diauxic shift show the following: 1) yeast lower cAMP levels as they exhaust their glucose supply and shift to oxidative metabolism of ethanol, 2) a reduction in cAMP is essential for traversing the diauxic shift, 3) the decrease in adenylate cyclase activity is associated with a decrease in the expression of CYR1 and CDC25, two positive regulators of cAMP levels and an increase in the expression of IRA1 and IRA2, two negative regulators of intracellular cAMP, 4) mutants carrying disruptions in IRA1 and IRA2 were unable to arrest cell division at the diauxic shift and were unable to progress into the oxidative phase of growth. These results indicate that changes cAMP levels are important in regulation of growth arrest at the diauxic shift and that changes in gene expression plays a role in the regulation of the Ras/adenylate cyclase system.

scholarly journals RESPON IMUNITAS BENIH LOBSTER, Panulirus homarus DENGAN PENGGUNAAN PROBIOTIK PADA PAKAN MOIST

2017 ◽  
Vol 12 (1) ◽  
pp. 85
Author(s):  
Haryanti Haryanti ◽  
Sari Budi Moria Sembiring ◽  
Sudewi Sudewi ◽  
Zeny Widiastuti ◽  
I Nyoman Adiasmara Giri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Defense Mechanism ◽  
Clotting System ◽  
Yeast Saccharomyces Cerevisiae ◽  
Individual System ◽  
Genes Expression ◽  
Clotting Protein

Pemeliharaan benih lobster P. homarus masih menghadapi beberapa permasalahan, di antaranya infeksi penyakit bakteri (red body disease) dan mortalitas yang tinggi. Tujuan penelitian ini adalah untuk mengkaji respons imunitas benih lobster P. homarus yang diberi pakan pelet basah (moist diets) dengan penambahan probiotik. Pemeliharaan benih lobster dilakukan secara individu (1 ekor/keranjang). Lama pemeliharaan selama tiga bulan. Bobot awal puerulus P. homarus adalah 0,37 ± 0,05 g. Perlakuan meliputi pemberian pakan moist yang ditambahkan (A) ragi Saccharomyces cerevisiae, (B) kombinasi probiotik, Alteromonas sp. BY-9 dan Bacillus cereus BC, dan (C) tanpa probiotik. Respons imunitas dianalisis dengan RT-qPCR melalui tujuh gen target terkait ekspresi imunitas, setelah diuji tantang dengan Vibrio harveyi (penyebab red body disease). Hasil penelitian menunjukkan bahwa sintasan benih lobster sebesar (A) 32,22%; (B) 29,63%; dan (C) 33,33%. Pertumbuhan panjang dan bobot benih lobster tidak berbeda nyata (P>0,05). Respons imunitas benih lobster P. homarus pada perlakuan A dan B menunjukkan nilai ekspresi imun yang lebih tinggi dibandingkan dengan perlakuan C (tanpa probiotik). Ekspresi gen penyandi anti lipopolisakarida (ALFHa-1) meningkat pada (A) rata-rata sebesar 3,44 kali dan (B) 3,25 kali dibandingkan dengan perlakuan C (2,43 kali). Kelipatan ekspresi profenoloksidase (proPO) benih lobster meningkat pada perlakuan A (penambahan ragi) rata-rata sebesar 5,27 kali, sedangkan pada perlakuan B (kombinasi probiotik) sebesar 12,92 kali. Ekspresi Clotting sistem (transglutaminase, clotting protein) dan antioxidant defense mechanism (glutathione peroxidase/GPO) dan SAA juga mengalami peningkatan pada perlakuan A dan B.A number of contrains including disease infections and significant mortality have been occurring in lobster aquaculture. The aim of this research was to observe the immune response of juvenile lobster P. homarus culture fed by moist pellet supplemented with probiotic. Experimental juveniles were reared in individual system (one juvenile/basket). The experiment was conducted for three months. The treatments comprised (A) whole cell of yeast Saccharomyces cerevisiae, (B) combination of probiotics Alteromonas sp. BY-9 and Bacillus sp. BC, and (C) without probiotic as control. Initial weight of juveniles were 0.37 ± 0.05 g. Immunity responses were analyzed using seven immunity related genes expression by RT-qPCR. The results showed that the survival rate of juvenile for treatments A, B, and C were 32.22%, 29.63%, and 33.33% respectively. The weight and length gain of the juvenile were not significantly different (P>0.05) among treatments. Based on immunity related gene expression analysis, it revealed that A and B treatments have shown differences in the increament of immunity responses. Expressions of ALFHa-1 genes were increased on (A) treatment with average of 3.44 fold and (B) treatment (3.25 fold) and higher than C treatment (2.03 fold). Prophenoloxidase (ProPO) expression was increase average up to 5.27 fold on A (yeast supplementated) treatment and B (combination of probiotic) were 12.92 fold. Gene expression on Clotting system (transglutaminase, clotting protein) and antioxidant defense mechanism (glutathione peroxidase/GPO) was increased on A and B treatments.

scholarly journals Mechanisms that ensure monogamous mating in Saccharomyces cerevisiae

2021 ◽  
pp. mbc.E20-12-0757
Author(s):  
Corrina G. Robertson ◽  
Manuella R. Clark-Cotton ◽  
Daniel J. Lew
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Multiple Mating ◽  
The Other ◽  
Specific Gene ◽  
Fusion Event ◽  
Yeast Saccharomyces Cerevisiae ◽  
Multiple Partners ◽  
Potential Partner

Haploid cells of the budding yeast Saccharomyces cerevisiae communicate using secreted pheromones and mate to form diploid zygotes. Mating is monogamous, resulting in the fusion of precisely one cell of each mating type. Monogamous mating in crowded conditions, where cells have access to more than one potential partner, raises the question of how multiple-mating outcomes are prevented. Here we identify mutants capable of mating with multiple partners, revealing the mechanisms that ensure monogamous mating. Before fusion, cells develop polarity foci oriented towards potential partners. Competition between these polarity foci within each cell leads to disassembly of all but one focus, thus favoring a single fusion event. Fusion promotes the formation of heterodimeric complexes between subunits that are uniquely expressed in each mating type. One complex shuts off haploid-specific gene expression, and the other shuts off the ability to respond to pheromone. Zygotes able to form either complex remain monogamous, but zygotes lacking both can re-mate.

Sign in / Sign up

Export Citation Format

Share Document