scholarly journals Young modulus of supported lipid membranes containing milk sphingomyelin in the gel, fluid or liquid-ordered phase, determined using AFM force spectroscopy

2019 ◽  
Vol 1861 (9) ◽  
pp. 1523-1532 ◽  
Author(s):  
Oumaima Et-Thakafy ◽  
Fanny Guyomarc'h ◽  
Christelle Lopez
2010 ◽  
Vol 73 (10) ◽  
pp. 965-972 ◽  
Author(s):  
C. Canale ◽  
M. Jacono ◽  
A. Diaspro ◽  
S. Dante

2014 ◽  
Vol 14 (5) ◽  
pp. 617-623 ◽  
Author(s):  
Michael Hirtz ◽  
Naresh Kumar ◽  
Lifeng Chi

Membranes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 11
Author(s):  
Damian Dziubak ◽  
Kamil Strzelak ◽  
Slawomir Sek

Supported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods used for preparation of planar lipid films, self-assembly of bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism of formation and the electrochemical properties of lipid films deposited onto thioglucose-modified gold electrodes from bicellar mixtures. It was found that adsorption of the bicelles occurs by replacement of interfacial water and it leads to formation of a double bilayer structure on the electrode surface. The resulting lipid assembly contains numerous defects and pinholes which affect the permeability of the membrane for ions and water. Significant improvement in morphology and electrochemical characteristics is achieved upon freeze–thaw treatment of the deposited membrane. The lipid assembly is rearranged to single bilayer configuration with locally occurring patches of the second bilayer, and the number of pinholes is substantially decreased. Electrochemical characterization of the lipid membrane after freeze–thaw treatment demonstrated that its permeability for ions and water is significantly reduced, which was manifested by the relatively high value of the membrane resistance.


1998 ◽  
Vol 10 (5) ◽  
pp. 295-302 ◽  
Author(s):  
Victor I. Passechnik ◽  
Tibor Hianik ◽  
Sergey A. Ivanov ◽  
Branislav Sivak

2018 ◽  
Vol 115 (13) ◽  
pp. 3255-3260 ◽  
Author(s):  
Xinxing Zhang ◽  
Kevin M. Barraza ◽  
J. L. Beauchamp

The role of cholesterol in bilayer and monolayer lipid membranes has been of great interest. On the biophysical front, cholesterol significantly increases the order of the lipid packing, lowers the membrane permeability, and maintains membrane fluidity by forming liquid-ordered–phase lipid rafts. However, direct observation of any influence on membrane chemistry related to these cholesterol-induced physical properties has been absent. Here we report that the addition of 30 mol % cholesterol to 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (POPG) monolayers at the air–water interface greatly reduces the oxidation and ester linkage cleavage chemistries initiated by potent chemicals such as OH radicals and HCl vapor, respectively. These results shed light on the indispensable chemoprotective function of cholesterol in lipid membranes. Another significant finding is that OH oxidation of unsaturated lipids generates Criegee intermediate, which is an important radical involved in many atmospheric processes.


RSC Advances ◽  
2016 ◽  
Vol 6 (94) ◽  
pp. 91102-91110 ◽  
Author(s):  
Fang Zhao ◽  
Jenny Perez Holmberg ◽  
Zareen Abbas ◽  
Rickard Frost ◽  
Tora Sirkka ◽  
...  

Different levels of model systems are needed for effect studies of engineered nanoparticles and the development of nanoparticle structure–activity relationships in biological systems.


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