Quantitative assessment of cellular uptake and cytosolic access of antibody in living cells by an enhanced split GFP complementation assay

2015 ◽  
Vol 467 (4) ◽  
pp. 771-777 ◽  
Author(s):  
Ji-sun Kim ◽  
Dong-Ki Choi ◽  
Seong-wook Park ◽  
Seung-Min Shin ◽  
Jeomil Bae ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Quantitative Assessment ◽  
Living Cells ◽  
Complementation Assay

scholarly journals GFP-complementation assay to detect functional CPP and protein delivery into living cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Nadia Milech ◽  
Brooke AC Longville ◽  
Paula T Cunningham ◽  
Marie N Scobie ◽  
Heique M Bogdawa ◽  
...  
Keyword(s):  
Protein Delivery ◽  
Living Cells ◽  
Complementation Assay

scholarly journals Quantitative assessment of ratiometric bimolecular beacons as a tool for imaging single engineered RNA transcripts and measuring gene expression in living cells

2013 ◽  
Vol 41 (15) ◽  
pp. e152-e152 ◽  
Author(s):  
Xuemei Zhang ◽  
Yang Song ◽  
Akash Y. Shah ◽  
Virzhiniya Lekova ◽  
Arjun Raj ◽  
...  
Keyword(s):  
Gene Expression ◽  
Quantitative Assessment ◽  
Living Cells ◽  
Rna Transcripts

Switchable two-photon imaging of RGD-functionalized polynorbornenes with enhanced cellular uptake in living cells

2016 ◽  
Vol 40 (4) ◽  
pp. 3252-3260 ◽  
Author(s):  
Nan Xie ◽  
Ke Feng ◽  
Bin Chen ◽  
Chen-Ho Tung ◽  
Li-Zhu Wu
Keyword(s):  
Cellular Uptake ◽  
Living Cells ◽  
Rgd Peptides ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

Two-photon imaging polynorbornenes were fabricated directly from photochromic spiropyran, RGD peptides and hydrophilic PEG monomers via modular ROMP.

scholarly journals Monitoring the Kinetics of the Cellular Uptake of a Metal Carbonyl Conjugated with a Lipidic Moiety in Living Cells Using Synchrotron Infrared Spectromicroscopy

2019 ◽  
Vol 74 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Sylvain Clède ◽  
Christophe Sandt ◽  
Paul Dumas ◽  
Clotilde Policar
Keyword(s):  
Breast Cancer ◽  
Cellular Uptake ◽  
Microfluidic Device ◽  
Metal Carbonyl ◽  
Living Cells ◽  
High Survival ◽  
High Survival Rate ◽  
Short Term ◽  
Kinetics Of ◽  
Single Living

Presented here is the exploitation of synchrotron infrared spectromicroscopy to evaluate the feasibility of monitoring the cellular uptake of rhenium-tris-carbonyl-tagged (Re(CO)3) lipophilic chains in living cells. To this aim, an in-house thermostated microfluidic device was used to limit water absorption while keeping cells alive. Indeed, cells showed a high survival rate in the microfluidic device over the course of the experiment, proving the short-term biocompatibility of the device. We recorded spectra of single, living, fully hydrated breast cancer MDA-MB231 cells and could follow the penetration of the rhenium complexes for up to 2 h. Despite the strong variations observed in the uptake kinetics between individual cells, the Re(CO)3 complex was traced inside the cells at low concentration and shown to enter them on the hour time scale by active transport.

scholarly journals Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution

2010 ◽  
Vol 24 (3-4) ◽  
pp. 303-307 ◽  
Author(s):  
Petr Praus ◽  
Eva Kocišová ◽  
Peter Mojzeš ◽  
Josef Štepánek ◽  
Franck Sureau ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Decay Time ◽  
Spectral Region ◽  
High Frequency ◽  
Antisense Oligonucleotide ◽  
Drug Distribution ◽  
Image Intensifier ◽  
Living Cells ◽  
Time Data ◽  
Time Resolved

Time-resolved confocal microspectrofluorometry and fluorescence microimaging were used to monitor how the model antisense oligonucleotide is transported into 3T3 living cells and distributed inside them. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with Zn(II) 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin as an uptake-mediating agent. Homodyne phase-resolved technique based on a high frequency analog modulation of both exciting diode laser and detector image intensifier was used for time-resolved measurements. Decay-time data obtained within a broad range spectral region have provided unique information about the fate of both fluorophores inside the cell.

Mechanism of Cellular Uptake to Optimized AuNP Beacon for Tracing mRNA Changes in Living Cells

2017 ◽  
Vol 35 (2) ◽  
pp. 1700331 ◽  
Author(s):  
Siwen Li ◽  
Caiping Tian ◽  
Yuxi Liu ◽  
Zhaohui Wang ◽  
Yi Ma ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Living Cells

Bioimaging, cellular uptake and dynamics in living cells of a lipophilic fluorescent benzothiadiazole at low temperature (4 °C)

2014 ◽  
Vol 5 (10) ◽  
pp. 3995 ◽  
Author(s):  
Alberto A. R. Mota ◽  
Pedro H. P. R. Carvalho ◽  
Bruna C. Guido ◽  
Heibbe C. B. de Oliveira ◽  
Thereza A. Soares ◽  
...  
Keyword(s):  
Low Temperature ◽  
Cellular Uptake ◽  
Living Cells
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