scholarly journals Monitoring the Kinetics of the Cellular Uptake of a Metal Carbonyl Conjugated with a Lipidic Moiety in Living Cells Using Synchrotron Infrared Spectromicroscopy

2019 ◽  
Vol 74 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Sylvain Clède ◽  
Christophe Sandt ◽  
Paul Dumas ◽  
Clotilde Policar
Keyword(s):  
Breast Cancer ◽  
Cellular Uptake ◽  
Microfluidic Device ◽  
Metal Carbonyl ◽  
Living Cells ◽  
High Survival ◽  
High Survival Rate ◽  
Short Term ◽  
Kinetics Of ◽  
Single Living

Presented here is the exploitation of synchrotron infrared spectromicroscopy to evaluate the feasibility of monitoring the cellular uptake of rhenium-tris-carbonyl-tagged (Re(CO)3) lipophilic chains in living cells. To this aim, an in-house thermostated microfluidic device was used to limit water absorption while keeping cells alive. Indeed, cells showed a high survival rate in the microfluidic device over the course of the experiment, proving the short-term biocompatibility of the device. We recorded spectra of single, living, fully hydrated breast cancer MDA-MB231 cells and could follow the penetration of the rhenium complexes for up to 2 h. Despite the strong variations observed in the uptake kinetics between individual cells, the Re(CO)3 complex was traced inside the cells at low concentration and shown to enter them on the hour time scale by active transport.

NF-κB activation in single living cells — Analysis of anti-apoptosis and kinetics of activation by IL-1β

1999 ◽  
Vol 27 (3) ◽  
pp. A94-A94
Author(s):  
Franço Carlotti ◽  
Lin Yang ◽  
Steven K. Dower ◽  
Eva E. Qwarnstrom
Keyword(s):  
Living Cells ◽  
Single Living Cells ◽  
Kinetics Of ◽  
Single Living

scholarly journals The Rate of Proflavin Passage into Single Living Cells with Application to Permeability Studies

1960 ◽  
Vol 43 (4) ◽  
pp. 853-866 ◽  
Author(s):  
Elliott Robbins
Keyword(s):  
Tissue Culture ◽  
Physicochemical Properties ◽  
Single Cell ◽  
Uptake Rate ◽  
Living Cells ◽  
Dye Transport ◽  
Permeability Studies ◽  
Single Living Cells ◽  
Kinetics Of ◽  
Single Living

A technique for the measurement of the uptake rate of proflavin by the single cell in tissue culture has been developed, and the kinetics of the dye transport are discussed in terms of its physicochemical properties. Some applications of the technique to the study of permeability are given.

Viable rabbits derived from oocytes by intracytoplasmic injection of spermatozoa from an infertile male

Zygote ◽  
2009 ◽  
Vol 17 (2) ◽  
pp. 157-162 ◽  
Author(s):  
Qiuyan Li ◽  
Jian Hou ◽  
Sheng Wang ◽  
Hong Guan ◽  
Nan Zhang ◽  
...  
Keyword(s):  
Early Stage ◽  
White Male ◽  
High Survival ◽  
High Survival Rate ◽  
Infertile Male ◽  
Intracytoplasmic Injection ◽  
Limited Success ◽  
Single Living ◽  
Assisted Fertilization

SummaryIntracytoplasmic sperm injection (ICSI) is an assisted fertilization technique and has been widely applied in human medicine to overcome some obstacles of infertility. However, this technology has not yet been used as a mainstream technique for animal production, including the rabbit, due to its limited success. The aim of this study was to improve ICSI techniques and establish an efficient ICSI method for rabbits. Spermatozoa used for ICSI were collected from mature New Zealand white male rabbits. They were washed two to three times with HEPES-buffered TCM199 containing 10% FBS and then mixed with 10% polyvinylpyrrolidone (PVP) prior to microinjection. Oocytes were harvested from superovulated donor rabbits after 14–15 h hCG treatment and were fertilized by microinjection of a single living spermatozoon into the ooplasm of each oocyte without additional activation treatment. A total of 317 injected oocytes resulted in the high survival rate of 86.1%. Among the surviving oocytes, 273 were placed into culture dishes for in vitro development. The fertilization, cleavage and blastocyst rates were 59.0%, 88.2% and 45.3% respectively. Furthermore, ICSI embryos were produced with spermatozoa from an infertile male rabbit, and 21 early-stage embryos (2-cell and 4-cell) were surgically transferred into the oviducts of two adult female rabbits. On day 31 after transfer, one out of the two recipients gave birth to two normal and healthy young rabbits. These results demonstrate that rabbit oocytes can be successfully activated and fertilized by the new ICSI protocol. Spermatozoa derived from infertile rabbits can successful fertilize oocytes and produce offspring by the simple ICSI technique.

scholarly journals Breast Cancer as a late complication of treatment for lymphoma in children and adolescents

10.3823/2426 ◽  
2017 ◽  
Vol 10 ◽  
Author(s):  
Antonio Marlos Duarte De Melo ◽  
Jose Angelo Araújo Sampaio ◽  
Ricardo Souto Quidute
Keyword(s):  
Breast Cancer ◽  
Survival Rate ◽  
Hodgkin’S Lymphoma ◽  
Breast Tissue ◽  
Pediatric Patients ◽  
Late Complication ◽  
Hodgkin's Lymphoma ◽  
High Survival ◽  
High Survival Rate ◽  
Secondary Neoplasms

The treatment for pediatric patients affected by Hodgkin's lymphoma, including chemotherapy and radiotherapy, has offered a high survival rate, but may be responsible for the appearing of breast cancer in later form in these same people. The 20-year survival rate can reach 90% of patients suffering from Hodgkin's disease, but they may suffer sequelae, among which malignant secondary neoplasms, including breast cancer. Most studies show that the malignancy of breast tissue is the main secondary sequel to radiotherapeutics’ treatment for patients with Hodgkin's lymphoma in childhood.   Keywords: Breast Cancer; Hodgkin's Lymphoma; Radiotherapy.

scholarly journals Label-free measuring and mapping of binding kinetics of membrane proteins in single living cells

2012 ◽  
Vol 4 (10) ◽  
pp. 846-853 ◽  
Author(s):  
Wei Wang ◽  
Yunze Yang ◽  
Shaopeng Wang ◽  
Vinay J. Nagaraj ◽  
Qiang Liu ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Living Cells ◽  
Binding Kinetics ◽  
Label Free ◽  
Single Living Cells ◽  
Kinetics Of ◽  
Single Living

scholarly journals Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorinfin Human Breast Cancer Cells: A Diffusion Kinetics Study

2012 ◽  
Vol 2012 ◽  
pp. 1-8
Author(s):  
Ping Chen ◽  
Feng Zhang ◽  
Lei Zhang ◽  
Song-Cheng Mao ◽  
Lie Lin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Diffusion Kinetics ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Kinetics Of ◽  
Mcf 7

The kinetics of photosensitizer 2-devinyl-2-(1-methoxylethyl) chlorinf(CPD4) uptake in MCF-7 human breast cancer cells is described by a diffusion kinetics model and experimentally investigated using laser scanning confocal microscopy (LSCM). CPD4 permeated into MCF-7 cells with increasing incubation time, which was followed by its binding to cell organelles. Subcellular distribution study revealed that CPD4 was primarily localized on the mitochondria and membranes, supporting that the mode of transmembrane transport was diffusion. A kinetics model describing CPD4 passing through the plasma membrane of MCF-7 cells was proposed based on Fick's first law of diffusion. The kinetics of cellular uptake of CPD4 was studied by three-dimensional LSCM. By fitting the experimental data using the above model, important cellular uptake and distribution parameters were obtained, which are of clinical significance in photodynamic therapy.

scholarly journals The successful use of fentanyl/fluanisone ('Hypnorm') as an anaesthetic for intracranial surgery in neonatal rats

2000 ◽  
Vol 34 (3) ◽  
pp. 260-264 ◽  
Author(s):  
Gavin J. Clowry ◽  
Paul A. Flecknell
Keyword(s):  
High Survival ◽  
High Survival Rate ◽  
Neonatal Rats ◽  
Short Term ◽  
Depth Of Anaesthesia ◽  
Fentanyl Citrate ◽  
Intracranial Surgery ◽  
Old Rats ◽  
Very High

This study reports on the successful use of fentanyl citrate and fluanisone ('Hypnorm') anaesthesia for intracranial surgery in neonatal (7-day-old) rats. Provided the anaesthetic was administered subcutaneously, the animals showed a very high survival rate in the short term (81/85, 95%) and showed no ill effects in the long term. The depth of anaesthesia was sufficient to allow the operation to be carried out without the animal reacting to any painful stimuli. However, the animals did make random movements during the period of surgical anaesthesia which were not related to any painful stimuli. Although these movements did not interfere with the surgery performed here, such movements would interfere with operations requiring greater precision, such as the localized micro-injection of neural tracers.

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