Pyruvate diminishes the cytotoxic activity of ascorbic acid in several tumor cell lines in vitro

EucalyptusL. is traditionally used for many medicinal purposes. In particular, someEucalyptusspecies have currently shown cytotoxic properties. Local Brazilian communities have used leaves ofE. benthamiias a herbal remedy for various diseases, including cancer. Considering the lack of available data for supporting this cytotoxic effect, the goal of this paper was to study thein vitrocytotoxic potential of the essential oils from young and adult leaves ofE. benthamiiand some related terpenes (α-pinene, terpinen-4-ol, andγ-terpinene) on Jurkat, J774A.1 and HeLa cells lines. Regarding the cytotoxic activity based on MTT assay, the essential oils showed improved results thanα-pinene andγ-terpinene, particularly for Jurkat and HeLa cell lines. Terpinen-4-ol revealed a cytotoxic effect against Jurkat cells similar to that observed for volatile oils. The results of LDH activity indicated that cytotoxic activity of samples against Jurkat cells probably involved cell death by apoptosis. The decrease of cell DNA content was demonstrated due to inhibition of Jurkat cells proliferation by samples as a result of cytotoxicity. In general, the essential oils from young and adult leaves ofE. benthamiipresented cytotoxicity against the investigated tumor cell lines which confirms their antitumor potential.

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Statins potentiate cytostatic/cytotoxic activity of sorafenib but not sunitinib against tumor cell lines in vitro

Cancer Letters ◽

10.1016/j.canlet.2009.06.022 ◽

2010 ◽

Vol 288 (1) ◽

pp. 57-67 ◽

Cited By ~ 22

Author(s):

Jacek Bil ◽

Lukasz Zapala ◽

Dominika Nowis ◽

Marek Jakobisiak ◽

Jakub Golab

Keyword(s):

Tumor Cell ◽

Cytotoxic Activity ◽

Cell Lines ◽

Tumor Cell Lines

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The ability to overcome multidrug resistance of tumor cell lines by novel acridine cytostatics with condensed heterocyclic rings.

Acta Biochimica Polonica ◽

10.18388/abp.2002_3824 ◽

2002 ◽

Vol 49 (1) ◽

pp. 87-92 ◽

Cited By ~ 7

Author(s):

Maria M Bontemps-Gracz ◽

Agnieszka Kupiec ◽

Ippolito Antonini ◽

Edward Borowski

Keyword(s):

Multidrug Resistance ◽

Tumor Cell ◽

Cytotoxic Activity ◽

Cell Lines ◽

Human Tumor ◽

Heterocyclic Ring ◽

Tumor Cell Lines ◽

Human Tumor Cell Lines

Two recently synthesized groups of acridine cytostatics containing fused heterocyclic ring(s): pyrazoloacridines (PAC) and pyrazolopyrimidoacridines (PPAC) were tested in regard to their in vitro cytotoxic activity towards a panel of sensitive and resistant human tumor cell lines. The obtained results corroborate our earlier hypothesis on the essential role of heterocyclic ring fused to the acridine moiety in the ability of acridine cytostatics to overcome multidrug resistance of tumor cells. The presence, location and kind of substituents considerably influenced both the cytotoxic activity of the derivatives and their ability to overcome multidrug resistance. The same factors also affected the cytostatics ability to differentiate between tumor cell lines with various types of drug exporting pumps.

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ChemInform Abstract: Synthesis and in vitro Cytotoxic Activity of Novel Hexahydro-2H-pyrido[1,2-b]isoquinolines Against Human Brain Tumor Cell Lines.

ChemInform ◽

10.1002/chin.199812120 ◽

2010 ◽

Vol 29 (12) ◽

pp. no-no

Author(s):

A. MONSEES ◽

S. LASCHAT ◽

M. HOTFILDER ◽

J. WOLFF ◽

K. BERGANDER ◽

...

Keyword(s):

Brain Tumor ◽

Tumor Cell ◽

Human Brain ◽

Cytotoxic Activity ◽

Cell Lines ◽

Tumor Cell Lines ◽

Human Brain Tumor ◽

Brain Tumor Cell

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Five New Meroterpenoids from the Fruiting Bodies of the Basidiomycete Clitocybe clavipes with Cytotoxic Activity

Molecules ◽

10.3390/molecules24224015 ◽

2019 ◽

Vol 24 (22) ◽

pp. 4015 ◽

Cited By ~ 3

Author(s):

Zhaocui ◽

Xudong ◽

Hanqiao ◽

Xinyi ◽

Guoxu ◽

...

Keyword(s):

Tumor Cell ◽

Cytotoxic Activity ◽

Cell Lines ◽

Human Tumor ◽

Tumor Cell Lines ◽

Fruiting Bodies ◽

Human Tumor Cell ◽

Human Tumor Cell Lines ◽

Ic50 Values

Five new meroterpenoids, clavipols A–B (1–2) with a 12-membered ether ring and clavilactones G–I (3–5) having a 10-membered carbocycle connected to a hydroquinone and an α,β-epoxy/unsaturated lactone, were obtained from the fruiting bodies of the basidiomycete Clitocybe clavipes. Their structures were determined by comprehensive analysis of their spectroscopic data, and the absolute configuration of 1 was established by quantum chemical calculations of electronic circular dichroism (ECD). All the isolated compounds (1–5) were tested for their cytotoxic activity against three human tumor cell lines (Hela, SGC-7901, and SHG-44) in vitro after treatment for 48 h. Compound 4 exhibited moderate cytotoxic activity against Hela and SGC-7901 tumor cell lines, with IC50 values of 23.5 and 14.5 µM, respectively.

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297 RAV12: a glycotope-specific chimeric antibody that exhibits potent cytotoxic activity against gastrointestinal tumor cell lines in vitro and in vivo

European Journal of Cancer Supplements ◽

10.1016/s1359-6349(04)80305-7 ◽

2004 ◽

Vol 2 (8) ◽

pp. 91

Author(s):

N.K. Pryer ◽

D. Loo ◽

P. Young ◽

R. Winter ◽

K. King ◽

...

Keyword(s):

Tumor Cell ◽

Cytotoxic Activity ◽

Cell Lines ◽

Chimeric Antibody ◽

Tumor Cell Lines ◽

Gastrointestinal Tumor

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A Lipoaminopeptaibol Secreted by Alkalophilic Fungus Emericellopsis alkalina Demonstrates a Strong Cytotoxic Effect against Tumor Cell Lines

Proceedings ◽

10.3390/proceedings2019022004 ◽

2019 ◽

Vol 22 (1) ◽

pp. 4

Author(s):

Eugene Rogozhin ◽

Vera Sadykova

Keyword(s):

Tumor Cell ◽

Cytotoxic Activity ◽

Cell Lines ◽

Cytotoxic Effect ◽

Antimicrobial Effect ◽

Antitumor Antibiotic ◽

Tumor Cell Lines ◽

Normal Cells ◽

Hela Cell Lines

Soil fungi are known to produce and secrete antibiotics with a strong antimicrobial effect towards eukaryotic organisms. In many occasions, these compounds belong to peptides that are products of non-ribosomal biosynthesis and are called peptaibols. Many peptaibols are cytotoxic and some of them suppress tumor cell lines much better than normal cells by inducing calcium-mediated apoptosis. The main antimicrobial lipoaminopeptaibol—emericellipsin A—isolated from the fungus Emericellopsis alkalina strain VKPM F-1428, which demonstrates promising antifungal activity against different fungal taxons,has been found to exhibit selective cytotoxic activity against HepG2 and Hela cell lines (EC50 2.8 and 0.5 μM, respectively) in MTT assays in vitro. This result corresponds to the standard antitumor antibiotic doxorubicin, which has an EC50 value of 440 nM. In a fibroblast toxicity test, emericellipsin A exhibited less cytotoxic activity than doxorubicin (EC50 14 and 0.34 μM, respectively). Therefore, it is less toxic to normal cells than doxirubicin (~40 times), but it yields a more potent cytotoxic effect on tumor cell lines. That is why emericellipsin A can be considered for future more detailed investigations to be an effective antitumor substance.

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Synthesis and Cytotoxicity of New Mannich Bases of 6-[3-(3,4,5-Trimetoxyphenyl)-2- propenoyl]-2(3H)-Benzoxazolone

Letters in Drug Design & Discovery ◽

10.2174/1570180816666190730164952 ◽

2020 ◽

Vol 17 (4) ◽

pp. 512-517

Author(s):

Ognyan Ivanov Petrov ◽

Yordanka Borisova Ivanova ◽

Mariana Stefanova Gerova ◽

Georgi Tsvetanov Momekov

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Biological Activities ◽

Human Tumor ◽

Mannich Bases ◽

In Vitro Cytotoxicity ◽

Tumor Cell Lines ◽

Human Tumor Cell ◽

Human Tumor Cell Lines

Background: Chemotherapy is one of the mainstays of cancer treatment, despite the serious side effects of the clinically available anticancer drugs. In recent years increasing attention has been directed towards novel agents with improved efficacy and selectivity. Compounds with chalcone backbone have been reported to possess various biological activities such as anticancer, antimicrobial, anti-inflammatory, analgesic, antioxidant, etc. It was reported that aminomethylation of hydroxy chalcones to the corresponding Mannich bases increased their cytotoxicity. In this context, our interest has been focused on the design and synthesis of the so-called multi-target molecules, containing two or more pharmacophore fragments. Methods: A series of Mannich bases were synthesized by the reaction between 6-[3-(3,4,5- trimethoxyphenyl)-2-propenoyl]-2(3Н)-benzoxazolone, formaldehyde, and a secondary amine. The structures of the compounds were confirmed by elemental analysis, IR and NMR spectra. The new Mannich bases were evaluated for their in vitro cytotoxicity against a panel of human tumor cell lines, including BV-173, SKW-3, K-562, HL-60, HD-MY-Z and MDA-MB-231. The effects of selected compounds on the cellular levels of glutathione (GSH) were determined. Results: The new compounds 4a-e exhibited concentration-dependent cytotoxic effects at micromolar concentrations in MTT-dye reduction assay against a panel of human tumor cell lines, similar to those of starting chalcone 3. The tested agents led to concentration - dependent depletion of cellular GSH levels, whereby the effects of the chalcone prototype 3 and its Mannich base-derivatives were comparable. Conclusion: The highest chemosensitivity to the tested compounds was observed in BV- 173followed by SKW-3 and HL-60 cell lines.

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