297 RAV12: a glycotope-specific chimeric antibody that exhibits potent cytotoxic activity against gastrointestinal tumor cell lines in vitro and in vivo

EucalyptusL. is traditionally used for many medicinal purposes. In particular, someEucalyptusspecies have currently shown cytotoxic properties. Local Brazilian communities have used leaves ofE. benthamiias a herbal remedy for various diseases, including cancer. Considering the lack of available data for supporting this cytotoxic effect, the goal of this paper was to study thein vitrocytotoxic potential of the essential oils from young and adult leaves ofE. benthamiiand some related terpenes (α-pinene, terpinen-4-ol, andγ-terpinene) on Jurkat, J774A.1 and HeLa cells lines. Regarding the cytotoxic activity based on MTT assay, the essential oils showed improved results thanα-pinene andγ-terpinene, particularly for Jurkat and HeLa cell lines. Terpinen-4-ol revealed a cytotoxic effect against Jurkat cells similar to that observed for volatile oils. The results of LDH activity indicated that cytotoxic activity of samples against Jurkat cells probably involved cell death by apoptosis. The decrease of cell DNA content was demonstrated due to inhibition of Jurkat cells proliferation by samples as a result of cytotoxicity. In general, the essential oils from young and adult leaves ofE. benthamiipresented cytotoxicity against the investigated tumor cell lines which confirms their antitumor potential.

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Tumorigenicity of simian virus 40-hepatocyte cell lines: effect of in vitro and in vivo passage on expression of liver-specific genes and oncogenes.

Molecular and Cellular Biology ◽

10.1128/mcb.8.10.4492 ◽

1988 ◽

Vol 8 (10) ◽

pp. 4492-4501 ◽

Cited By ~ 36

Author(s):

C D Woodworth ◽

J W Kreider ◽

L Mengel ◽

T Miller ◽

Y L Meng ◽

...

Keyword(s):

Gene Expression ◽

Tumor Cell ◽

Cell Lines ◽

Phosphoenolpyruvate Carboxykinase ◽

Simian Virus 40 ◽

Simian Virus ◽

Tumor Cell Lines ◽

Glutathione S Transferase

Five simian virus 40 (SV40)-hepatocyte cell lines were examined for tumorigenicity and the effect of in vitro passage on the expression of four liver-specific genes (albumin, transferrin, alpha 1-antitrypsin, and phosphoenolpyruvate carboxykinase), two oncogenes (c-Ha-ras and c-raf), and two genes associated with hepatocarcinogenesis (alpha-fetoprotein and placental-type glutathione-S-transferase). At low passage (12 to 22), all five cell lines expressed the four liver-specific genes at levels similar to those in the liver and were not tumorigenic or were weakly tumorigenic. At high passage (33 to 61), the cell lines formed carcinomas, and four out of five cell lines produced primary tumors that metastasized. At least two cell lines produced well-differentiated hepatocellular carcinomas that expressed liver-specific RNAs. Levels of expression of liver-specific genes changed with time in culture. Some of the changes in liver-specific gene expression in the tumor tissue (such as for the phosphoenolpyruvate carboxykinase gene) paralleled those that occurred with in vitro passage, while other changes (such as for the albumin gene) did not parallel those that occurred with in vitro passage. Correlations between enhanced expression of c-Ha-ras and tumorigenic potential and between the process of SV40 immortalization and induced expression of c-raf and glutathione-S-transferase-P were observed. Induction of alpha-fetoprotein was detected with in vitro and in vivo passage only in the CWSV14 cell line and was paralleled by diminished albumin expression. In conclusion, we developed a model system with five SV40-hepatocyte cell lines, tumors induced by them, and tumor cell lines to examine changes in gene expression that accompany the progression from a normal cell to a hepatocellular carcinoma. Because the SV40-hepatocyte cell lines and tumor cell lines remain highly differentiated and vary in the magnitude of expression of specific genes, they can be used to study the molecular mechanisms regulating gene expression, in particular those regulating specific genes associated with differentiation.

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Statins potentiate cytostatic/cytotoxic activity of sorafenib but not sunitinib against tumor cell lines in vitro

Cancer Letters ◽

10.1016/j.canlet.2009.06.022 ◽

2010 ◽

Vol 288 (1) ◽

pp. 57-67 ◽

Cited By ~ 22

Author(s):

Jacek Bil ◽

Lukasz Zapala ◽

Dominika Nowis ◽

Marek Jakobisiak ◽

Jakub Golab

Keyword(s):

Tumor Cell ◽

Cytotoxic Activity ◽

Cell Lines ◽

Tumor Cell Lines

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Inhibition of Tumor Cell Proliferation In Vitro by Benzamide Derivatives

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.997.225 ◽

2014 ◽

Vol 997 ◽

pp. 225-228 ◽

Cited By ~ 1

Author(s):

Yan Ling Wu ◽

Li Wen Shen ◽

Yan Ping Ding ◽

Yoshimasa Tanaka ◽

Wen Zhang

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Malignant Tumors ◽

Dependent Manner ◽

Tumor Cell Lines ◽

Lead Compounds ◽

Proliferative Effect ◽

Benzamide Derivatives

Benzamide derivatives have been shown to have antitumor activity in various tumor cell lines in vitro as well as in vivo. In this study, we examined the anti-proliferative effect of four benzamide derivativeson Hela, H7402, and SK-RC-42 tumor cell lines in vitro by means of Real-Time cell assay (RTCA), and found that four benzamide derivatives suppressed proliferation of tumor cells in a time-and dose-dependent manner. The anti-proliferative activity of benzamide derivatives demonstrated that theycould be promising lead compounds for developing therapeutic agents for malignant tumors.

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The ability to overcome multidrug resistance of tumor cell lines by novel acridine cytostatics with condensed heterocyclic rings.

Acta Biochimica Polonica ◽

10.18388/abp.2002_3824 ◽

2002 ◽

Vol 49 (1) ◽

pp. 87-92 ◽

Cited By ~ 7

Author(s):

Maria M Bontemps-Gracz ◽

Agnieszka Kupiec ◽

Ippolito Antonini ◽

Edward Borowski

Keyword(s):

Multidrug Resistance ◽

Tumor Cell ◽

Cytotoxic Activity ◽

Cell Lines ◽

Human Tumor ◽

Heterocyclic Ring ◽

Tumor Cell Lines ◽

Human Tumor Cell Lines

Two recently synthesized groups of acridine cytostatics containing fused heterocyclic ring(s): pyrazoloacridines (PAC) and pyrazolopyrimidoacridines (PPAC) were tested in regard to their in vitro cytotoxic activity towards a panel of sensitive and resistant human tumor cell lines. The obtained results corroborate our earlier hypothesis on the essential role of heterocyclic ring fused to the acridine moiety in the ability of acridine cytostatics to overcome multidrug resistance of tumor cells. The presence, location and kind of substituents considerably influenced both the cytotoxic activity of the derivatives and their ability to overcome multidrug resistance. The same factors also affected the cytostatics ability to differentiate between tumor cell lines with various types of drug exporting pumps.

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In Vitro and in Vivo Selection of two Lewis Lung Carcinoma Cell Lines

Tumori Journal ◽

10.1177/030089167906500601 ◽

1979 ◽

Vol 65 (6) ◽

pp. 657-664 ◽

Cited By ~ 15

Author(s):

Ada Sacchi ◽

Anna Corsi ◽

Marco Caputo ◽

Gabriella Zupi

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Lung Carcinoma ◽

Lewis Lung Carcinoma ◽

Lung Metastases ◽

Tumor Cell Lines ◽

Lewis Lung ◽

Selection Of

Two tumor cell lines adapted to grow in vitro were originated from an explant of lung metastases of Lewis lung carcinoma. These lines differ in their malignancy when reinoculated into syngeneic animals; nevertheless, they do not show any difference for their in vitro clonogenic ability. From these lines 2 in vivo sublines of 3LL carcinoma were developed. The TD 50 of the 2 in vivo sublines are different, and both the values obtained are lower than that of the original line. These results are interpreted as a selection of more malignant tumor cell lines.

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