Removal notice to “Bioactive Paper Design for Human Blood Analysis: Paper Property Suitable for Large-scale Sensor Production” [Biochem Eng J (2016) 473–480]

2016 ◽  
Vol 105 ◽  
pp. R1
Author(s):  
Liyun Guan ◽  
Lizi Li ◽  
Xiaolei Huang ◽  
Jianfeng Ji ◽  
Junfei Tian ◽  
...  
Keyword(s):  
Human Blood ◽  
Large Scale ◽  
Blood Analysis ◽  
Bioactive Paper ◽  
Paper Property

REMOVED: Bioactive Paper Design for Human Blood Analysis: Paper Property Suitable for Large-scale Sensor Production

2016 ◽  
Vol 105 ◽  
pp. 473
Author(s):  
Liyun Guan ◽  
Lizi Li ◽  
Xiaolei Huang ◽  
Jianfeng Ji ◽  
Junfei Tian ◽  
...  
Keyword(s):  
Human Blood ◽  
Large Scale ◽  
Blood Analysis ◽  
Bioactive Paper ◽  
Paper Property

scholarly journals Using a Two-Sample Mendelian Randomization Method in Assessing the Causal Relationships Between Human Blood Metabolites and Heart Failure

2021 ◽  
Vol 8 ◽  
Author(s):  
Zixian Wang ◽  
Shiyu Chen ◽  
Qian Zhu ◽  
Yonglin Wu ◽  
Guifeng Xu ◽  
...  
Keyword(s):  
Heart Failure ◽  
Human Blood ◽  
Large Scale ◽  
Genome Wide Association Study ◽  
Mendelian Randomization ◽  
Causal Effect ◽  
Meta Analysis ◽  
Sensitivity Analyses ◽  
Blood Metabolites ◽  
Causal Relationships

Background: Heart failure (HF) is the main cause of morbidity and mortality worldwide, and metabolic dysfunction is an important factor related to HF pathogenesis and development. However, the causal effect of blood metabolites on HF remains unclear.Objectives: Our chief aim is to investigate the causal relationships between human blood metabolites and HF risk.Methods: We used an unbiased two-sample Mendelian randomization (MR) approach to assess the causal relationships between 486 human blood metabolites and HF risk. Exposure information was obtained from Sample 1, which is the largest metabolome-based genome-wide association study (mGWAS) data containing 7,824 Europeans. Outcome information was obtained from Sample 2, which is based on the results of a large-scale GWAS meta-analysis of HF and contains 47,309 cases and 930,014 controls of Europeans. The inverse variance weighted (IVW) model was used as the primary two-sample MR analysis method and followed the sensitivity analyses, including heterogeneity test, horizontal pleiotropy test, and leave-one-out analysis.Results: We observed that 11 known metabolites were potentially related to the risk of HF after using the IVW method (P < 0.05). After adding another four MR models and performing sensitivity analyses, we found a 1-SD increase in the xenobiotics 4-vinylphenol sulfate was associated with ~22% higher risk of HF (OR [95%CI], 1.22 [1.07–1.38]).Conclusions: We revealed that the 4-vinylphenol sulfate may nominally increase the risk of HF by 22% after using a two-sample MR approach. Our findings may provide novel insights into the pathogenesis underlying HF and novel strategies for HF prevention.

scholarly journals Monoclonal Antibody-Based Time-Resolved Fluorescence Immunoassays for Daidzein, Genistein, and Equol in Blood and Urine: Application to the Isoheart Intervention Study

2007 ◽  
Vol 53 (4) ◽  
pp. 748-756 ◽  
Author(s):  
Duncan CS Talbot ◽  
Richard M Ogborne ◽  
Tony Dadd ◽  
Herman Adlercreutz ◽  
Geoff Barnard ◽  
...  
Keyword(s):  
Large Scale ◽  
Intervention Studies ◽  
Intervention Study ◽  
Dietary Intervention ◽  
Blood Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Ether Extraction ◽  
Dietary Intervention Trial

Abstract Background: Time-resolved fluorescence immunoassays (TR-FIAs) for phytoestrogens in biological samples are an alternative to mass spectrometric methods. These immunoassays were used to test urine and plasma samples from individuals in a dietary intervention trial aimed at determining the efficacy of dietary isoflavones in reducing the risk of coronary heart disease in postmenopausal women. Methods: We established murine monoclonal TR-FIA methods for daidzein, genistein, and equol. These assays could be performed manually or adapted to an automated analyzer for high throughput and increased accuracy. Analysis of urine was conducted on nonextracted samples. Blood analysis was performed on nonextracted samples for daidzein, whereas genistein and equol required diethyl-ether extraction. Results: Comparison of monoclonal TR-FIA, commercial polyclonal antibody–based TR-FIA, and gas chromatography–mass spectrometry showed correlations (r, 0.911–0.994) across the concentration range observed in the Isoheart study (50 mg/day isoflavones). The concentrations of urinary daidzein and genistein observed during intervention demonstrated good compliance, and a corresponding increase in serum daidzein and genistein confirmed bioavailability of the isoflavone-rich foods; 33 of the 117 volunteers (28.2%) were classified as equol producers on the basis of their urinary equol concentration (>936 nmol/L), and significant differences in the numbers of equol producers were observed between Berlin and the 3 other European cohorts studied. Conclusions: The validated monoclonal TR-FIA methods are applicable for use in large-scale human phytoestrogen intervention studies and can be used to monitor compliance, demonstrate bioavailability, and assess equol producer status.

scholarly journals Quantitative interferometric measurements of the refractive index and its applications

2021 ◽  
Author(s):  
Mohammed Yahya
Keyword(s):  
Refractive Index ◽  
Optical Properties ◽  
Human Blood ◽  
Medical Science ◽  
Medical Diagnostics ◽  
Blood Analysis ◽  
Optical Systems ◽  
Refractive Indices ◽  
Contact Method

Optical interferometry is a non-contact method used for the imaging and measurement of the optical properties of small or large objects with nanoscale accuracy. Interferometers are essential optical systems in engineering and medical science and are generally used to measure refractive index changes, cell pathology and shape irregularities. The ability to provide quantitative, non-invasive measurements makes interferometers suitable candidates for the study of living cells in vivo and in vitro applications. In this study, a simple interferometric system capable of providing precise measurements of the refractive indices of transparent and semi-transparent mediums was designed and developed based on the Mach-Zehnder arrangement. During the development phase, the system was used to take precise measurements of the contrast factors of the three hydrocarbon components 1,2,3,4-Tetrtahydronaphtalenene (THN), Isobutylbenzen (IBB), and Dodecane (nC12), prepared in the laboratory. The results were also compared with the corresponding results obtained using the Abbemat refractometer. The results were in agreement with those obtained from both techniques as well as the results obtained from the literature, confirming the accuracy of the measurements obtained with the new system with deviations of ± 2.50×10-3. Blood analysis is a routine procedure used in medical diagnostics to confirm a patient’s condition. Measuring the optical properties of blood is difficult due to the non-homogenous nature of the blood itself. In addition, there is a lot of variation in the refractive indices reported in the literature. These are the reasons that motivated this research to develop an empirical model that can be used to predict the refractive index of human blood as a function of concentration, temperature and wavelength. The experimental measurements were conducted on mimicking phantom, that shows better stability compared to human blood, using the Abbemat Refractometer. The values obtained using the model was in substantial agreement with those obtained experimentally with standard deviations of ± 2.33×10-3. Once the accuracy of the interferometric system was confirmed, the system was used to study the refractive index and morphology of human red blood cells. The results of the analysis confirmed the system’s ability to determine refractive index and/or blood hematocrit values with appropriate clinical accuracy.

THE ISOLATION OF COAGULATION FACTOR VIII FROM HUMAN BLOOD PLASMA BY AFFINITY CHROMATOGRAPHY

1987 ◽  
Author(s):  
W Riethorst ◽  
M W P M te Booy ◽  
T Beugeling ◽  
A Bantjes ◽  
J Over ◽  
...  
Keyword(s):  
Factor Viii ◽  
Blood Plasma ◽  
Human Blood ◽  
Large Scale ◽  
Room Temperature ◽  
Hydrophobic Interactions ◽  
Specific Activity ◽  
Coagulation Factor ◽  
Coagulation Factor Viii ◽  
Human Blood Plasma

The need for high quality concentrates of coagulation factor VIII (FVIII:C) for treatment of haemophilia A is increasing. As the purity of FVIII:C obtained with existing large scale methods is poor and yields are low, another method for the isolation of FVIII is being developed primarily to avoid losses incurred during cryoprecipitation.Affinity gels were prepared by derivatizing Sepharose CL 4B with different positively charged ligand-spacer combinations. The adsorption of FVIII as well as the von Willebrand factor (VWF) from human blood plasma onto these gels was measured by a one-stage assay for FVIII:C, and enzyme immuno assays (ELISA) for FVIII:CAG and VWF:AG using monoclonal antibodies. The influences of pH, conductivity, ligand density, geltplasma ratio, and length and composition of the spacer as well as the adsorption kinetics were studied to obtain information about the types of interactions responsible for bonding of FVIII to the gels. A combination of at least electrostatic and hydrophobic interactions was concluded to play a role in most cases.At optimal conditions more than 90 % of FVIII could be adsorbed batch-wise from plasma at room temperature in less than one hour with a gel:plasma ratio of 1:20 (i.e. 2.5 g dried gel/1 plasma). In different runs 65-75 % of the FVIII:C applied was recovered by column-wise elution with a salt gradient. The eluate contained less than 0.34 % of the protein applied, which implies that FVIII was purified 190 times. Using fresh-frozen plasma (0.8 IU FVIII/ml) as a starting material for this one-step procedure the final specific activity was 2.3 IU/mg, which is significantly better than that obtained for FVIII isolated by cryoprecipitation. Furthermore, the F. VIII:C to VWF ratio in the eluate was approximately 1:1. The isolated FVIII:C was stable at room temperature and the supernatant plasma appears suitable for further fractionation. It is concluded that this method is worth scaling up and its use for purification of FVIII from other sources is anticipated.

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