scholarly journals Nucleocapsid protein of SARS-CoV activates the expression of cyclooxygenase-2 by binding directly to regulatory elements for nuclear factor-kappa B and CCAAT/enhancer binding protein

2006 ◽  
Vol 38 (8) ◽  
pp. 1417-1428 ◽  
Author(s):  
Xiaohong Yan ◽  
Qian Hao ◽  
Yongxin Mu ◽  
Khalid Amine Timani ◽  
Linbai Ye ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Nuclear Factor Kappa B ◽  
Binding Protein ◽  
Regulatory Elements ◽  
Cyclooxygenase 2 ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein

The role of the CCAAT/enhancer-binding protein in the transcriptional regulation of the gene for phosphoenolpyruvate carboxykinase (GTP)

1990 ◽  
Vol 10 (12) ◽  
pp. 6264-6272
Author(s):  
E A Park ◽  
W J Roesler ◽  
J Liu ◽  
D J Klemm ◽  
A L Gurney ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Binding Protein ◽  
Regulatory Elements ◽  
Specific Protein ◽  
Affinity Column ◽  
Binding Domains ◽  
Nuclear Extracts ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein

Previous studies have identified a region in the promoter of the gene for phosphoenolpyruvate carboxykinase (GTP) (PEPCK) (positions -460 to +73) containing the regulatory elements which respond to cyclic AMP, glucocorticoids, and insulin and confer the tissue- and developmental stage-specific properties to the gene. We report that CCAAT/enhancer-binding protein (C/EBP) binds to the cyclic AMP-responsive element CRE-1 as well as to two regions which have been previously shown to bind proteins enriched in liver nuclei. The DNase I footprint pattern provided by the recombinant C/EBP was identical to that produced by a 43-kDa protein purified from rat liver nuclear extracts, using a CRE oligonucleotide affinity column, which was originally thought to be the CRE-binding protein CREB. Transient contransfection experiments using a C/EBP expression vector demonstrated that C/EBP could trans activate the PEPCK promoter. The trans activation occurred through both the upstream, liver-specific protein-binding domains and the CRE. The CRE-binding protein bound only to CRE-1 and not to the upstream C/EBP-binding sites. The results of this study, along with physiological properties of C/EBP, indicate an important role for this transcription factor in providing the PEPCK gene with several of its regulatory characteristics.

Sign in / Sign up

Export Citation Format

Share Document