Field efficacy of the entomopathogenic nematode Steinernema thermophilum Ganguly and Singh (Rhabditida: Steinernematidae) against diamondback moth (Plutella xylostella L.) infesting cabbage

2006 ◽  
Vol 37 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Vishal Singh Somvanshi ◽  
Sudershan Ganguly ◽  
A.V.N. Paul
Keyword(s):  
Plutella Xylostella ◽  
Entomopathogenic Nematode ◽  
Diamondback Moth ◽  
Field Efficacy

scholarly journals Impact of insecticides on the efficacy of entomopathogenic nematodes against the diamondback moth Plutella xylostella (L.) (Lepidoptera: Plutellidae) on cabbage in northern Benin

2019 ◽  
Author(s):  
Brice G. Sinhouenon ◽  
Hugues Baimey ◽  
Laura Wauters ◽  
Rufin Dossou ◽  
Regis B. Ahissou ◽  
...  
Keyword(s):  
Survival Rate ◽  
Plutella Xylostella ◽  
Entomopathogenic Nematodes ◽  
Entomopathogenic Nematode ◽  
Larval Mortality ◽  
Diamondback Moth ◽  
Neem Oil ◽  
Nematode Reproduction ◽  
The Subject ◽  
The Impact

Description of the subject. The article deals with the study of compatibility of entomopathogenic nematode (EPN) species with insecticides currently used against diamondback moth (DBM) Plutella xylostella (L.) in northern Benin Objectives. The aim of this work was to determine the impact of five insecticides on the efficacy of entomopathogenic nematodes against DBM larvae. Method. Infective juveniles (IJs) of two EPN species (Steinernema sp. 83a and Heterorhabditis sonorensis KF723827) were exposed to five insecticides used against P. xylostella larvae in Djougou for 48 h. The number of surviving nematodes was used to infest DBM larvae. The experiment was carried out under laboratory and semi-field conditions. Results. The bioassays carried out in laboratory showed that the survival rate of nematodes exposed to KARATE 2.5 WG, LAMBDA SUPER 2.5EC or neem oil ranges between 95% and 98%. In the treated plots, Steinernema sp. 83a was the most virulent with the highest P. xylostella mortality (87% at 50 IJs·cm-2 after 24 h) while only 35% larval mortality was recorded for H. sonorensis applied at the same dose. Population density of nematodes which penetrated DBM larvae reached 9 ± 3I IJs·larva-1 for H. sonorensis KF723827 and 6 ± 2 IJs·larva-1 for Steinernema sp. 83a. In cadaver of DBM, nematode reproduction did not appear to be affected by the contact with insecticides. Conclusions. Based on our research, we conclude that the three insecticides did not affect EPNs efficiency and could be used in combination against DBM.

scholarly journals Efficacy of Two Entomopathogenic Nematode Species as Potential Biocontrol Agents against the Diamondback Moth, Plutella xylostella (L.)

2016 ◽  
Vol 30 (2) ◽  
pp. 78
Author(s):  
Masaaod Zolfagharian ◽  
Ayatollah Saeedizadeh ◽  
Habib Abbasipour
Keyword(s):  
Plutella Xylostella ◽  
Laboratory Experiments ◽  
Entomopathogenic Nematodes ◽  
Entomopathogenic Nematode ◽  
Larval Mortality ◽  
Diamondback Moth ◽  
Nematode Species ◽  
Steinernema Carpocapsae ◽  
Important Pest ◽  
High Larval Mortality

The diamondback moth, <em>Plutella xylostella</em> (L.) (Lepidoptera: Plutellidae) is an important pest of cruciferous crops in Iran. The susceptibility of <em>P. xylostella</em> larvae to two species of entomopathogenic nematodes (EPNs) (<em>Steinernema carpocapsae</em> and <em>Heterorhabditis bacteriophora</em>) was examined under laboratory conditions. Leaf bioassays were conducted to evaluate the nematode's capability to reach the larvae and kill them. High larval mortality (72.6-96%) was observed in laboratory experiments. The ET<sub>50</sub> of <em>H. bacteriophora</em> was higher than that of <em>S. carpocapsae</em>. The ET<sub>50</sub> of entomopatpgenic nematodes, <em>H. bacteriophora</em> and <em>S. carpocapsae</em> tested ranged from 21 to 139.7 and 11.3 to 71.4 hours, respectively. The effect of both factors infective juveniles (IJs) and exposure time of 50% (ET50) on the larval mortality was significant (df = 6; P &lt; 0.001) and (df = 2; P &lt; 0.001), respectively. This study revealed that entomopatogenic nematodes (EPNs) have great potential that should be exploited in diamondback moth, <em>P. xylostella</em> management.

Evaluation of adjuvants for foliar application of Steinernema carpocapsae against larvae of the diamondback moth (Plutella xylostella)

Nematology ◽  
2005 ◽  
Vol 7 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Sibylle Schroer ◽  
Xiaoli Yi ◽  
Ralf-Udo Ehlers
Keyword(s):  
Plutella Xylostella ◽  
Foliar Application ◽  
Entomopathogenic Nematode ◽  
Larval Instar ◽  
Diamondback Moth ◽  
Fold Increase ◽  
Steinernema Carpocapsae ◽  
Control Measures ◽  
The Third ◽  
Insect Mortality

Abstract Increasing resistance to chemical insecticides in field populations of the diamondback moth (Plutella xylostella) has stimulated research on alternative control measures. The entomopathogenic nematode Steinernema carpocapsae may be one such alternative, particularly against the third larval instar of P. xylostella. The LC50 for the second instar is 38, the third 13 and the fourth 22 nematodes/larva. Plutella xylostella pupae were not affected by the nematodes, although mortality in leaf disk bioassays after application of nematodes in water seldom surpassed 50%. Therefore, additives were tested to improve nematode performance. Only Triton X-100 (0.3%) caused phytotoxic effects. The addition of xanthan gum or potassium alginate resulted in a two-fold increase of insect mortality at 80% relative humidity (RH) and a five-fold increase at 60% RH. Mixtures of 0.3% xanthan or alginate with 0.3% surfactants further improved efficacy. In water the LT50 for S. carpocapsae against P. xylostella larvae was > 40 h. Using a mixture of 0.3% xanthan or 0.3% alginate with 0.3% surfactant, the LT50 was reduced to < 25 h.

Efficacy of entomopathogenic nematode,Steinernema carpocapsaeagainst the diamondback moth,Plutella xylostella(L.) in laboratory condition

2014 ◽  
Vol 48 (5) ◽  
pp. 393-399 ◽  
Author(s):  
Masaaod Zolfagharian ◽  
Ayatallah Saeedizadeh ◽  
Habib Abbasipour ◽  
Ali Joyandeh ◽  
Ali Ahmadian Yazdi
Keyword(s):  
Laboratory Condition ◽  
Plutella Xylostella ◽  
Entomopathogenic Nematode ◽  
Diamondback Moth

Effects of rhizobacteria on the biology and behavior of Plutella xylostella (Lepidoptera: Plutellidae)

2017 ◽  
Vol 43 (2) ◽  
pp. 195
Author(s):  
Robson Thomaz Thuler ◽  
Fernando Henrique Iost Filho ◽  
Hamilton César De Oliveira Charlo ◽  
Sergio Antônio De Bortoli
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Insect Behavior ◽  
Control Treatment ◽  
Pupal Weight ◽  
Larval Feeding ◽  
Direct Effects ◽  
Plant Feeding ◽  
Kluyvera Ascorbata ◽  
And Behavior

Plant induced resistance is a tool for integrated pest management, aimed at increasing plant defense against stress, which is compatible with other techniques. Rhizobacteria act in the plant through metabolic changes and may have direct effects on plant-feeding insects. The objective of this study was to determine the effects of cabbage plants inoculated with rhizobacteria on the biology and behavior of diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Cabbage seeds inoculated with 12 rhizobacteria strains were sowed in polystyrene trays and later transplanted into the greenhouse. The cabbage plants with sufficient size to support stress were then infested with diamondback moth caterpillars. Later, healthy leaves suffering injuries were collected and taken to the laboratory to feed P. xylostella second instar caterpillars that were evaluated for larval and pupal viability and duration, pupal weight, and sex ratio. The reduction of leaf area was then calculated as a measure of the amount of larval feeding. Non-preference for feeding and oviposition assays were also performed, by comparing the control treatment and plants inoculated with different rhizobacterial strains. Plants inoculated with the strains EN4 of Kluyvera ascorbata and HPF14 of Bacillus thuringiensis negatively affected the biological characteristics of P. xylostella when such traits were evaluated together, without directly affecting the insect behavior.

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