Fortified bacterial consortium – A novel approach to control root knot nematode in cucumber (cucumis sativum)

2020 ◽  
pp. 104528
Author(s):  
D.G. Panpatte ◽  
H.N. Shelat ◽  
Y.K. Jhala ◽  
R.V. Vyas
Keyword(s):  
Bacterial Consortium ◽  
Root Knot Nematode ◽  
Novel Approach ◽  
Control Root

scholarly journals The Application of Manure (Poultry Wests) and Bio-Formulations of Trichoderma harzianum and T. viride and Their Interaction to Control Root-knot Disease on Radish and Chard

2019 ◽  
Vol 32 (1) ◽  
pp. 88-98
Author(s):  
Nuha A. Al-Zehebawi ◽  
Dhia S. Al-Waily ◽  
Labeed A. Al-Saad
Keyword(s):  
Plant Height ◽  
Molecular Identification ◽  
Contaminated Soil ◽  
Trichoderma Harzianum ◽  
Control Treatment ◽  
Root Knot Nematode ◽  
Green House ◽  
Control Root ◽  
Positive Treatment

The study was designed to examine the effect of manure (poultry wastes) and bio-formulations of Trichoderma harzianum and T. viride separately or with some, to control root-knot disease on radish (Raphanus sativus L.) and chard (Beta vulagaris var.cicla (L.)). The study included the isolation of pathogenic nematode of both plant roots, morphological and molecular identification, examination of the pathogenicity in vitro and in vivo and green house experiments involved application of manure and fungal bio-formulations treatments. The morphological and molecular identification confirmed the identity of root-knot nematode, as Meloidogyne javanica, which was pathogenic to radish and chard. The recorded infection severity was 89 and 95% respectively. The green house experiment results revealed that MThTv treatment was significantly reduced infection severity to 0% for radish and chard in contaminated soil (CS) compared with control positive treatment (55.17 and 40%) respectively. MThTv treatment also showed a highest plant height for Radish in non-nematode-contaminated soil (NCS) and CS treatment (17.85 and 16.50 cm) respectively compared with control positive treatment (5.00 cm), while the highest plant height of Chard was 24.5 cm in MThTv-NCS. The wet weight index in Radish showed a superiority of MThTv and MTh in NC on other treatments (201.75 and 189.5 g.plant-1) respectively followed by MThTv-NCS treatment (184.5 g.plant-1) compared with 19.25 gm.plant-1 in control treatment. In Chard the results showed similar pattern represented by superiority of MThTv-NC treatment (255.25 gm.plant-1) followed by MThTv-NCS (190.75 gm.plant-1) compared with 37.50 gm/plant for positive control.

scholarly journals Exploiting cell cycle inhibitor genes of the KRP family to control root-knot nematode induced feeding sites in plants

2017 ◽  
Vol 40 (7) ◽  
pp. 1174-1188 ◽  
Author(s):  
Roberta Ramos Coelho ◽  
Paulo Vieira ◽  
José Dijair Antonino de Souza Júnior ◽  
Cristina Martin-Jimenez ◽  
Lieven De Veylder ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Root Knot Nematode ◽  
Feeding Sites ◽  
Cell Cycle Inhibitor ◽  
Control Root ◽  
Inhibitor Genes

scholarly journals Trichoderma asperellum affects Meloidogyne incognita infestation and development in Celosia argentea

2020 ◽  
Vol 5 (1) ◽  
pp. 778-784
Author(s):  
Alori Elizabeth Temitope ◽  
Aluko Ajibola Patrick ◽  
Joseph Abiodun ◽  
Adekiya Aruna Olasekan ◽  
Aremu Charity Onye ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Plant Root ◽  
Trichoderma Asperellum ◽  
Root Knot Nematode ◽  
Environmental Consequences ◽  
Control Root ◽  
Biocontrol Activity ◽  
Celosia Argentea ◽  
Gall Index ◽  
Plant Root System

AbstractDue to the rise in cost and detrimental health and environmental consequences that accompany the use of nematicides, there is a need for a more eco-friendly and less expensive alternative to control root-knot nematode (Meloidogyne incognita). Nematode infestation reduces the quality and quantity of Celosia argentea Linn. A pot experiment was conducted in a greenhouse to determine the biocontrol efficacy of Trichoderma asperellum against M. incognita in C. argentea. The treatments consisted M. incognita infected C. argentea inoculated with 0, 2.2 × 107, 4.4 × 107, or 6.6 × 107 cfu/pot of T. asperellum. All doses of T. asperellum reduced the root-knot nematode population and root gall index. Growth and development of C. argentea were improved, indicating that T. asperellum has the potential to be used as a biocontrol agent in C. argentea production. The biocontrol activity of T. asperellum in C. argantea increased as the week went by until the plants attained full maturity. Hence, the control of M. incognita by T. asperellum depends on the developmental stage of the plant root system.

scholarly journals The synergistic advantage of combining chloropicrin or dazomet with fosthiazate nematicide to control root-knot nematode in cucumber production

2019 ◽  
Vol 18 (9) ◽  
pp. 2093-2106 ◽  
Author(s):  
Bin HUANG ◽  
Qian WANG ◽  
Mei-xia GUO ◽  
Wen-sheng FANG ◽  
Xiao-ning WANG ◽  
...  
Keyword(s):  
Root Knot Nematode ◽  
Control Root

Root Extracts of Plants to Control Root-Knot Nematode on Edible Soybean

2006 ◽  
Vol 12 (2) ◽  
pp. 5-12 ◽  
Author(s):  
A. A. Adegbite ◽  
S. O. Adesiyan
Keyword(s):  
Root Knot Nematode ◽  
Root Extracts ◽  
Control Root

scholarly journals Endophytic Bacterial Consortium Originated from Forestry Plant Roots and Their Nematicidal Activity against Meloidogyne incognita Infestation in Greenhouse

2019 ◽  
Vol 67 (5) ◽  
pp. 1171-1182
Author(s):  
Abdul Munif ◽  
Supramana ◽  
Elis Nina Herliyana ◽  
Ankardiansyah Pandu Pradana
Keyword(s):  
Meloidogyne Incognita ◽  
Volatile Compound ◽  
Bacterial Consortium ◽  
Physiological Characteristics ◽  
Phenotypic Characterization ◽  
Bacterial Isolates ◽  
Root Knot Nematode ◽  
Egg Hatching ◽  
Protease Enzyme

Yield loss due to root-knot nematode Meloidogyne incognita infection is reported to reach 35%, depends on factors contributing to infection. Application of several endophytic bacterial isolates (bacterial consortium) to control pathogenic infection is reported to be more effective compared to the application of single bacterial isolate. This study was aimed to obtain endophytic bacterial consortium originated from forestry plant that is effective to control root-knot nematode. The study was conducted through bacterial isolation followed by biosafety test. Bacterial isolates that were found to be safe for plants and mammals and compatible with each other were further grouped as the endophytic bacterial consortium. Phenotypic characterization and physiological characteristics including Gram type, ability to produce protease, chitinase, and lipase enzymes as well as HCN volatile compound were also tested. Moreover, the ability to fix nitrogen and dissolve phosphate were also examined. The endophytic bacterial consortium consisted of several bacterial isolates was further tested for its ability to inhibit M. incognita egg hatching and increase J2 of M. incognita mortality in vitro. Furthermore, test on tomato plants infested with 500 J2 of M. incognita was also performed in the greenhouse. Test results showed that 70 bacterial isolates were successfully isolated from Shorea sp., Swietenia sp., Albizia falcataria, Anthocephalus cadamba, and Juglans nigra. However, 34 bacterial isolates were observed to be safe (did not cause hypersensitivity reaction and did not produce hemolytic toxin). According to physiological characteristics, it was found that 25 isolates were able to produce protease enzyme, 26 isolates were able to produce chitinase enzyme, and 14 isolates were able to produce lipase enzyme. Moreover, it was also detected that 11 isolates were able to produce HCN volatile compound, 23 isolates were able to fix nitrogen (N), and 24 isolates were able to dissolve phosphate (P). Endophytic bacterial consortium obtained in this study was also observed to be able to inhibit M. incognita egg hatching up to 81.33% and increase J2 of M. incognita mortality up to 85% compared to control. In addition, the application of endophytic bacterial consortium was also able to increase the growth of tomato plant infected with M. incognita, and suppress the severity of the root-knot disease. This study provided information that endophytic bacterial consortium originated from forestry plants has the potential as a biocontrol agent of M. incognita.

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