scholarly journals Trichoderma asperellum affects Meloidogyne incognita infestation and development in Celosia argentea

2020 ◽  
Vol 5 (1) ◽  
pp. 778-784
Author(s):  
Alori Elizabeth Temitope ◽  
Aluko Ajibola Patrick ◽  
Joseph Abiodun ◽  
Adekiya Aruna Olasekan ◽  
Aremu Charity Onye ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Plant Root ◽  
Trichoderma Asperellum ◽  
Root Knot Nematode ◽  
Environmental Consequences ◽  
Control Root ◽  
Biocontrol Activity ◽  
Celosia Argentea ◽  
Gall Index ◽  
Plant Root System

AbstractDue to the rise in cost and detrimental health and environmental consequences that accompany the use of nematicides, there is a need for a more eco-friendly and less expensive alternative to control root-knot nematode (Meloidogyne incognita). Nematode infestation reduces the quality and quantity of Celosia argentea Linn. A pot experiment was conducted in a greenhouse to determine the biocontrol efficacy of Trichoderma asperellum against M. incognita in C. argentea. The treatments consisted M. incognita infected C. argentea inoculated with 0, 2.2 × 107, 4.4 × 107, or 6.6 × 107 cfu/pot of T. asperellum. All doses of T. asperellum reduced the root-knot nematode population and root gall index. Growth and development of C. argentea were improved, indicating that T. asperellum has the potential to be used as a biocontrol agent in C. argentea production. The biocontrol activity of T. asperellum in C. argantea increased as the week went by until the plants attained full maturity. Hence, the control of M. incognita by T. asperellum depends on the developmental stage of the plant root system.

scholarly journals Inoculum Level and Inoculation Method Influences on the Pathogenic Activities of Meloidogyne incognita in Studied Model Plant Okra (Abelmoschus esculentus L. Moench)

2021 ◽  
pp. 82-92
Author(s):  
Yadom Y. F. R. Kouakou ◽  
Kouamé Daniel Kra ◽  
Hortense Atta Diallo
Keyword(s):  
Meloidogyne Incognita ◽  
Infected Plant ◽  
Correlation Coefficients ◽  
Root Knot Nematode ◽  
Reproductive Factor ◽  
Inoculum Level ◽  
Root Explants ◽  
Second Stage ◽  
Inoculation Method ◽  
Gall Index

Agricultural activities such as watering crops with nematode-infested water from wells and boreholes, and using infected plant debris as manure or mulch increase root-knot nematode infection. So, this study aims at assessing the influence of the inoculation method and inoculum level of Meloidogyne incognita on the development of root galls on okra plants. Two M. incognita inoculation methods (suspension of individuals and galled root explants) and six inoculum levels (0, 10, 100, 500, 1000 and 2000 second-stage larvae/plant) were studied. The gall index, total numbers and reproductive factor of M. incognita were used to assess the effect of treatments on root gall development. Unlike the reproductive factor, gall index and the total numbers of M. incognita increased with their inoculum level. The pathogenic activities of M. incognita were most significant when crop soils were infested with galled root explants. However, an inverse relationship was found between the inoculum levels of M. incognita and the okra plant’s development. It is reflected by negative correlation coefficients ranging from -0.90 to -0.62. It is therefore important to burn roots infected with root-knot nematodes left in fields so that they do not act as an inoculum for crops.

scholarly journals Chitosan Oligosaccharide Fluorinated Derivative Control Root-Knot Nematode (Meloidogyne incognita) Disease Based on the Multi-Efficacy Strategy

Marine Drugs ◽  
2020 ◽  
Vol 18 (5) ◽  
pp. 273 ◽  
Author(s):  
Zhaoqian Fan ◽  
Yukun Qin ◽  
Song Liu ◽  
Ronge Xing ◽  
Huahua Yu ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Plant Root ◽  
Photosynthetic Pigment ◽  
Good Control ◽  
Chitosan Oligosaccharide ◽  
Root Knot Nematode ◽  
Control Effect ◽  
Low Cytotoxicity

Plant root-knot nematode disease is a great agricultural problem and commercially available nematicides have the disadvantages of high toxicity and limited usage; thus, it is urgent to develop new nematicides derived from nature substances. In this study, a novel fluorinated derivative was synthesized by modifying chitosan oligosaccharide (COS) using the strategy of multiple functions. The derivatives were characterized by FTIR, NMR, elemental analysis, and TG/DTG. The activity assays show that the derivatives can effectively kill the second instar larvae of Meloidogyne incognita in vitro, among them, chitosan-thiadiazole-trifluorobutene (COSSZFB) perform high eggs hatching inhibitory activity. The derivatives can regulate plant growth (photosynthetic pigment), improve immunity (chitinase and β-1,3-glucanase), and show low cytotoxicity and phytotoxicity. According to the multi-functional activity, the derivatives exhibit a good control effect on plant root-knot nematode disease in vivo. The results demonstrate that the COS derivatives (especially fluorinated derivative) perform multiple activities and show the potential to be further evaluated as nematicides.

scholarly journals Efficacy Of Two Organic Amendments And A Nematicide To Manage Root-Knot Nematode (Meloidogyne Incognita) Of Tomato (Lycopersicon Esculentum L.)

1970 ◽  
Vol 36 (3) ◽  
pp. 477-486 ◽  
Author(s):  
MI Faruk ◽  
MI Rahman ◽  
MR Ali ◽  
MM Rahman ◽  
MMH Mustafa
Keyword(s):  
Effective Treatment ◽  
Meloidogyne Incognita ◽  
Organic Amendments ◽  
Fruit Yield ◽  
First Year ◽  
Root Knot Nematode ◽  
Tomato Seedlings ◽  
Gall Index ◽  
Second Year ◽  
Treatment Application

A field experiment was conducted in two consecutive years to find out the efficacy of poultry refuse (PR), mustard oilcake (MOC), and Furadan 5G for the management of root-knot disease (Meloidogyne incognita) of tomato. Soil was treated with PR @ 3 and 5 t/ha, MOC @ 0.3 and 0.6 t/ha 3 weeks before transplanting and Furadan 5G @ 40 kg/ha on the day of transplanting of tomato seedlings. PR @ 3 t/ha and MOC @0.3 t/ha were applied alone and also mixed with Furadan 5G @ 20 kg/ha. The soils of the experimental plots were inoculated with chopped severely galled (M incognita) roots of tomato at the time of treatment application. In both the years, considerable reduction in rootknot disease and increase in plant growth and fruit yield were achieved with different treatments with two organic materials applied alone or mixed with Furadan 5G. The most effective treatment was PR @ 3 t/ha + Furadan 5G @ 20 kg/ha followed by PR alone @ 5 t/ha. Efficacy of PR @ 3 t/ha and MOC @ 0.6 1/ha were also appreciable. In first year and second year, gall index values were 6.50 and 6.27 under control, respectively. The severity was reduced to 2.27-4.00 in first year and 1.73-4.07 in second year due to application of the four treatments. On the other hand, fruit yield under control was 50.9 t/ha at first year and 47.6 t/ha in second year. The highly effective four treatments increased fruit yield to 71.1-82.5 t/ha in first year and 60.8-82.0 t/ha in second year. The fruit yield of tomato was directly and linearly correlated with gall indices in tomato gall. Based on findings of the study PR @ 3 t/ha + Furadan @20 kg/ha and PR alone @ 5 t/ha were noted as effective treatment to manage root-knot disease of tomato. Keywords: Poultry refuse; mustard oilcake; Furadan; Meloidogjyne incognita; tomato. DOI: http://dx.doi.org/10.3329/bjar.v36i3.9275 BJAR 2011; 36(3): 477-486

scholarly journals Bioefficacy of Bacillus subtilis against root knot nematode Meloidogyne incognita (Kofoid and White) Chitwood in tomato

2015 ◽  
Vol 7 (2) ◽  
pp. 1012-1015
Author(s):  
Subhalaxmi Roy ◽  
Arun Rathod ◽  
Aniruddha Pramanik
Keyword(s):  
Bacillus Subtilis ◽  
Trichoderma Harzianum ◽  
Meloidogyne Incognita ◽  
Dry Weight ◽  
Control Agent ◽  
Seedling Height ◽  
Root Knot Nematode ◽  
Tomato Fruits ◽  
Highest Weight ◽  
Gall Index

An investigation was conducted for the management of root knot nematode Meloidogyne incognita (Kofoid and White) Chitwood infesting tomato through the application of bio-control agent like Bacillus subtilis, Trichoderma harzianum and Pseudomonas fluorescens. Experiment result revealed that minimum no. of galls/25seedlings (17.50) and maximum seedling height (27.6cm) were observed in Bacillus subtilis @50g/m2 in nursery bed + B. subtilis @ 5kg along with 2.5 tons of FYM/ ha. The highest weight/25seedlings (69.50g) was noticed in the B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha. The highest growth of the plant at 45 DAT (49.2cm) and at harvest (81.2cm) and maximum fresh (711.3g) and dry weight (265g) was found in B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha. B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha exhibited lowest gall index (1.2/plant) and highest reduction of nematode population and provided highest yield of tomato fruits (335.75q/ha).

scholarly journals F4 families of crispleaf lettuce with tolerance to early bolting and homozygous for resistance to Meloidogyne incognita race 1

2009 ◽  
Vol 27 (3) ◽  
pp. 335-339
Author(s):  
José Luiz S de Carvalho Filho ◽  
Luiz Antonio A Gomes ◽  
Felipe A Biguzzi ◽  
Wilson Roberto Maluf ◽  
Sindynara Ferreira
Keyword(s):  
Root System ◽  
Meloidogyne Incognita ◽  
Leaf Blade ◽  
Fresh Weight ◽  
Root Knot Nematode ◽  
Egg Masses ◽  
Grand Rapids ◽  
Race 1 ◽  
Gall Index

The objective of this study was to evaluate yield, commercial characteristics, tolerance to early bolting and resistance to the root-knot nematode Meloidogyne incognita race 1 in 25 F4 families of crispleaf lettuce, obtained out of crosses between cultivars Grand Rapids, Regina 71, and Verônica. In the first experiment, we evaluate leaf blade and borders characteristics, aboveground fresh weight, and number of days from sowing to the anthesis of first flower (tolerance to early bolting). In the second experiment, we evaluated the resistance to Meloidogyne incognita race 1 via gall index and number of galls and egg masses per root system. Five families had scores for leaf blade and borders similar to cultivars Verônica and Grand Rapids. Furthermore, 84% of the families were as tolerant to early bolting as cultivar Veronica, while 92% of the families were homozygous for resistance to Meloidogyne incognita race 1.

scholarly journals Susceptibility of Eight Herbs to Common Root-Knot Nematodes

2002 ◽  
Vol 20 (2) ◽  
pp. 101-103
Author(s):  
Jerry T. Walker
Keyword(s):  
Meloidogyne Incognita ◽  
Mass Production ◽  
Root Systems ◽  
Common Species ◽  
Egg Mass ◽  
Common Root ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Gall Index ◽  
Herb Species

Abstract Eight herb species were inoculated with two common species of root-knot nematode and grown for 2 months in a greenhouse. Root systems were examined for galls and egg mass production. All herb species were susceptible but developed fewer galls and had lower gall indices than Rutgers tomato. Burnet, chives, valerian and winter savory had few galls or low gall indices. Eggs were produced on all. Chamomile had a high gall index. Chicory, parsley, and sorrel had intermediate indices. Herbs were equally susceptible to the southern (Meloidogyne incognita) and peanut root-knot nematodes (M. arenaria), particularly at the highest inoculum densities. Mean dry weights of inoculated herbs were not always significantly less than the non-inoculated plants, suggesting that some herbs may be tolerant to root-knot nematodes.

scholarly journals SUSCEPTIBILITY AND TOLERANCE TO ROOT-KNOT NEMATODES (MELOIDOGYNE INCOGNITA) OF TELFAIRIA OCCIDENTALIS UNDER ORGANIC CONVERSION

2020 ◽  
Vol 4 (3) ◽  
pp. 26-31
Author(s):  
Jonathan Atungwu ◽  
O. O. Olabinjo ◽  
C. O. Eche ◽  
I. Tijjani
Keyword(s):  
Meloidogyne Incognita ◽  
Yield Loss ◽  
Block Design ◽  
Soil Nematode ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Nematode Reproduction ◽  
Plant Soil ◽  
Telfairia Occidentalis ◽  
Celosia Argentea

Three cultivars of Telfairia occidentalis were assessed for their resistance or otherwise to root-knot nematodes (Meloidogyne spp.) infection on the field. Seeds were extracted from the pods of the selected cultivars and planted on moist sawdust in nursery trays to raise seedlings for transplanting. The experiment was laid out in Randomized Complete Block Design and replicated thrice. Pre-plant soil samples were taken to estimate initial nematode populations. At seven days after transplanting, eggs of Meloidogyne incognita (Mi) were extracted from 60 day-old Celosia argentea and used for inoculation of the three cultivars of T. occidentalis at approximately 5000 eggs/stand. Plants were, thereafter, observed for vine length, vine girth and number of leaves from two weeks till 8 weeks after transplanting. Final soil nematode population and root gall indices were determined at 60-66 days after inoculation. Reproduction factor was determined and used along with the gall indices to rate the cultivars for resistance or susceptibility to Mi. Results showed that cultivars NHTo-020 and NHTo-030 were susceptible to root-knot nematode which implied that plants allowed nematode reproduction and also suffered yield loss while cultivar NHTo-010 was tolerant to nematode infestation meaning that nematode reproduction took place but the plant does not suffer yield loss. There was no significant (P>0.05) difference in the mean vine length and number of leaf irrespective of the inoculum while effect on the vine girth was significantly variable. The inoculated plants gave significantly better vegetative growth than the naturally infested plants which indicated genetic variability in the cultivars.

scholarly journals Biocontrol agents in the management of Meloidogyne incognita in tomato

2017 ◽  
Vol 47 (10) ◽  
Author(s):  
Juliana de Oliveira Silva ◽  
Marcus Vinícius Santana ◽  
Leonardo Levorato Freire ◽  
Bruno da Silva Ferreira ◽  
Mara Rúbia da Rocha
Keyword(s):  
Population Density ◽  
Meloidogyne Incognita ◽  
Biocontrol Agents ◽  
Egg Mass ◽  
Root Knot Nematode ◽  
Tomato Crop ◽  
Tomato Plants ◽  
Biological Products ◽  
Gall Index ◽  
Different Doses

ABSTRACT: Recognized as prominent among the principal pests of tomato crop, the root-knot nematode (Meloidogyne incognita) has generated much study with regards to control methods. This study aimed to assess the efficiency of biological products in decreasing the M. incognita population in tomato. Four experiments were conducted under greenhouse conditions. Two of these experiments had the purpose to determine the efficiency of NemOut™ (Bacillus subtilis + B. licheniformis + Trichoderma longibrachiatum), and the other two to assess the efficiency of other different biological products in M. incognita management. Tomato plants of the cultivar Santa Cruz Kada Giant were inoculated using 2000 eggs and J2 of M. incognita. In the first two experiments doses corresponding to 0, 4, 6, 8 and 10kg ha-1 of NemOut™ were applied after inoculation. In the second study, the treatments consisted of the use of biological products containing different other biocontrol agents. Assessments were made at 45 and 65 days after inoculation (DAI) to evaluate plant height, fresh shoot and fresh root weigh, gall index and egg mass index, population density and reproduction factor (RF). The NemOut™ doses reduced the M. incognita population density and RF at 45 DAI. The agent Pochonia chlamydosporia showed greater efficiency in controlling M. incognita on tomato. Biological products and the different doses of NemOut™ had no influence on the tomato plant development.

Effect of chitin compost and broth on biological control of Meloidogyne incognita on tomato (Lycopersicon esculentum Mill.)

Nematology ◽  
2005 ◽  
Vol 7 (1) ◽  
pp. 125-132 ◽  
Author(s):  
Rong De Jin ◽  
Joo Won Suh ◽  
Ro Dong Park ◽  
Yong Woong Kim ◽  
Hari B. Krishnan ◽  
...  
Keyword(s):  
Biological Control ◽  
Meloidogyne Incognita ◽  
Chitinase Activity ◽  
Parasitic Nematodes ◽  
Vegetable Crops ◽  
Root Knot Nematode ◽  
Tomato Root ◽  
Chitinolytic Bacteria ◽  
Gall Index ◽  
Amended Soil

Abstract Plant-parasitic nematodes are distributed worldwide and affect a broad range of important agronomic plant species. Chitinolytic bacteria were evaluated as potential biological control agents of the root-knot nematode, Meloidogyne incognita, on tomato. After transplantation of seedlings into pots containing soil amended with chitin compost, chitin broth, or respective controls, soil was inoculated with nematode eggs and infective second-stage juveniles (J2). Samples taken at 4, 6 and 8 weeks after inoculation indicated that fresh weights of plants did not vary between treatments or between treatments and controls. The gall index was lower in the plants grown in the chitin-amended soil at each time point. Activities of soil chitinase and β-1,3-glucanase were greater in those soils amended with chitin compost and chitin broth. Gall index of tomato root was negatively correlated with soil chitinase activity. Activities of tomato root chitinase and β-1,3-glucanase were higher in plants growing in non-chitin-amended soil at 6 and 8 weeks after nematode infestation. Chitinase activity in tomato root was positively correlated with the gall index of tomato root. The results indicate the potential of chitinase producing bacteria to alleviate nematode parasitism in important vegetable crops.

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