Biocontrol mechanisms of Trichoderma harzianum ITEM 3636 against peanut brown root rot caused by fusarium solani RC 386

Black root rot of faba bean is one of the most destructive diseases that causes up to 100% yield loss on farmers’ fields under severe conditions. As use of chemical fungicides for control of the disease is neither efficient nor economical, alternative options such as biological control need to be exploited. The antagonistic fungus Trichoderma harzianum was used as seed dressing or soil application treatment in combination with three varieties viz. Kasa (susceptible), Wolki (moderately resistant) and Wayu (resistant) in two consecutive cropping seasons in a sick plot. Each variety was sown with and without T. harzianum. There were a total of six treatments. The experimental design was randomized complete block design with three replications in a 2 x 3 factorial arrangement. Results showed that in the first year highly significant variations in the percentage of dead plants at harvesting stage were observed only due to varietal differences for soil treatment (P = 0.0001) and seed dressing (P< 0.0001) respectively. Percentage of dead plants of variety wayu was 26.1% and 30.8% in soil application and seed dressing treatments respectively. Similarly, 92.1% and 95.2% dead plants were observed for the susceptible variety Kassa in soil application and seed dressing treatments respectively. In the second year there was a significant interaction between varieties and Trichoderma treatments (P=0.0047, F=8.66, df= 2) with variety Wayu showing the least percentage of dead plants (56.58%) significantly differing from Kassa (93.86%) and Wolki (77.08%) in the soil treatments. It is concluded that use of T. harzianum in combination with a resistant variety such as Wayu is effective to reduce the incidence of faba bean root rot caused by Fusarium solani. Future research should focus on studying the effects of combining different Trichoderma spp. on the disease as integrated with resistant varieties.

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Bio-management of Citrus Nematode, Tylenchulus semipenetrans and Dry Root Rot Fungi, Fusarium solani under Laboratory and Field Conditions

Egyptian Journal of Agronematology ◽

10.21608/ejaj.2019.52577 ◽

2019 ◽

Vol 18 (2) ◽

pp. 118-128

Author(s):

Dina Ibrahim ◽

Ayat Ali ◽

Howida Metwaly

Keyword(s):

Fusarium Solani ◽

Root Rot ◽

Field Conditions ◽

Tylenchulus Semipenetrans ◽

Citrus Nematode

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In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

BMC Microbiology ◽

10.1186/s12866-021-02181-7 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Alemayehu Dugassa ◽

Tesfaye Alemu ◽

Yitbarek Woldehawariat

Keyword(s):

Pseudomonas Aeruginosa ◽

Pseudomonas Fluorescens ◽

Faba Bean ◽

Fusarium Solani ◽

Root Rot ◽

Mycelial Growth ◽

Biocontrol Agents ◽

Black Root Rot ◽

Root Rot Disease ◽

Rot Disease

Abstract Background Faba bean (Vicia faba L.) cultivation is highly challenged by faba bean black root rot disease (Fusarium solani) in high lands of Ethiopia. To ensure sustainable production of faba beans, searching for eco-friendly disease management options is necessary to curb the progress of the disease timely. The indigenous biocontrol agents that suit local environments may effectively strive with in-situ microorganisms and suppress local pathogen strains. This study aimed to screen antagonistic indigenous compatible Trichoderma and Pseudomonas strains against Fusarium solani. In the pathogenicity test, soil-filled pots were arranged in complete random block design and sown with health faba bean seeds. The effect of some fungicides was evaluated against Fusarium by food poisoning methods to compare with the biocontrol agents. The antagonistic efficacy of biocontrol agents and their compatibility was investigated on Potato dextrose agar medium. Results Fusarium solani AAUF51 strain caused an intense root rotting in faba bean plant. The effect of Mancozeb 80% WP at 300 ppm was comparable with Trichoderma and Pseudomonas strains against Fusarium. The mycelial growth of test the pathogen was significantly (P ≤ 0.05) reduced to 86.67 and 85.19% by Trichoderma harzianum AAUW1 and Trichoderma viridae AAUC22 strains in dual culture, respectively. The volatile metabolites of Pseudomonas aeruginosa AAUS31 (77.78%) found the most efficient in reducing mycelial growth of Fusarium followed by Pseudomonas fluorescens AAUPF62 (71.11%) strains. The cell-free culture filtrates of Pseudomonas fluorescens AAUPF62 and Pseudomonas aeruginosa AAUS31 were more efficient than the Trichoderma strain in reducing the growth of Fusarium isolates. There was no zone of inhibition recorded between Trichoderma harzianum AAUW1, Trichoderma viridae AAUC22, Pseudomonas aeruginosa AAUS31, and Pseudomonas fluorescens AAUPF62 strains, hence they were mutually compatible. Conclusions The compatible Trichoderma and Pseudomonas strains showed antagonistic potentiality that could be explored for faba bean protection against black root rot disease and might have a future dual application as biocontrol agents.

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Evidence for the heritability of resistance to brown root rot of alfalfa, caused byPhoma sclerotioides

Canadian Journal of Plant Pathology ◽

10.1080/07060660509507195 ◽

2005 ◽

Vol 27 (1) ◽

pp. 64-70 ◽

Cited By ~ 6

Author(s):

C.R. Hollingsworth ◽

F.A. Gray ◽

R.W. Groose

Keyword(s):

Root Rot ◽

Brown Root Rot

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Methyl Bromide Fumigation of Soil Infested by the Fungus Causing Brown Root Rot Complex of Tomatoes and by the Potato Cyst Nematode

Plant Pathology ◽

10.1111/j.1365-3059.1967.tb00394.x ◽

1967 ◽

Vol 16 (4) ◽

pp. 148-152 ◽

Cited By ~ 3

Author(s):

G. L. James ◽

N. G. M. Hague

Keyword(s):

Methyl Bromide ◽

Root Rot ◽

Cyst Nematode ◽

Potato Cyst Nematode ◽

Brown Root Rot

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The brown root rot fungus Phellinus noxius affects microbial communities in different root‐associated niches of Ficus trees

Environmental Microbiology ◽

10.1111/1462-2920.15862 ◽

2021 ◽

Author(s):

Tse‐Yen Liu ◽

Chao‐Han Chen ◽

Yu‐Liang Yang ◽

Isheng J. Tsai ◽

Ying‐Ning Ho ◽

...

Keyword(s):

Microbial Communities ◽

Root Rot ◽

Brown Root Rot ◽

Phellinus Noxius

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Antifungal activity of Trichoderma harzianum and T. koningiopsis against Fusarium solani in seed germination and vigor of Miahuateco chili seedlings

Revista Mexicana de Fitopatología Mexican Journal of Phytopathology ◽

10.18781/r.mex.fit.2101-5 ◽

2021 ◽

Vol 39 (2) ◽

Author(s):

Leydi Miguel-Ferrer ◽

Omar Romero-Arenas ◽

Petra Andrade-Hoyos ◽

Primo Sánchez-Morales ◽

José Antonio Rivera-Tapia ◽

...

Keyword(s):

Antifungal Activity ◽

Seed Germination ◽

Trichoderma Harzianum ◽

Fusarium Solani ◽

Para Determinar ◽

Santa Maria

El chile es la segunda hortaliza de mayor producción en México. El objetivo de la investigación fue evaluar la actividad antagónica in vitro e in vivo de Trichoderma harzianum (T-H4) y T. koningiopsis (T-K11) versus Fusarium solani (MX-MIC 798) en la germinación y establecimiento de plántula de chile Miahuateco. Se utilizó la técnica de cultivo dual para determinar el porcentaje de inhibición de crecimiento radial (PICR) de la cepa MX-MIC 798. Además, se analizó el porcentaje de germinación en semillas de chile Miahuateco en vivero, así como la mortalidad de plántulas y grado de severidad a los 40 días después del trasplante (ddt) en Santa María Tecomavaca, Oaxaca, a través de biocontrol y control químico (Mancozeb 80®). La cepa T-H4 presentó el nivel antagónico PICR más alto (53.3%) in vitro y clase II en la escala de Bell, asimismo obtuvo 82% de germinación en semillas de chile Miahuateco en vivero y 48% de mortalidad en campo; de manera que igualó al control químico y superó a T. koningiopsis T-K11. La actividad antifúngica de Trichoderma spp., ofrecen una alternativa para el biocontrol de la marchitez y necrosis en raíz del cultivo de chile Miahuateco causada por F. solani MX-MIC 798.

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