Biopriming chilli seeds with Trichoderma asperellum: a study on biopolymer compatibility with seed and biocontrol agent for disease suppression

2021 ◽  
pp. 104819
Author(s):  
Jia May Chin ◽  
Yau Yan Lim ◽  
Adeline Su Yien Ting
Keyword(s):  
Biocontrol Agent ◽  
Disease Suppression ◽  
Trichoderma Asperellum

scholarly journals Biological and Application-Oriented Factors Influencing Plant Disease Suppression by Biological Control: A Meta-Analytical Review

2006 ◽  
Vol 96 (11) ◽  
pp. 1168-1174 ◽  
Author(s):  
P. S. Ojiambo ◽  
H. Scherm
Keyword(s):  
Biological Control ◽  
Effect Size ◽  
Untreated Control ◽  
Plant Disease ◽  
Biocontrol Agent ◽  
Disease Suppression ◽  
Effect Sizes ◽  
Biological Control Agents ◽  
Control Effect ◽  
Soilborne Diseases

Studies to evaluate the effectiveness of biological control in suppressing plant disease often report inconsistent results, highlighting the need to identify general factors that influence the success or failure of biological control in plant pathology. We conducted a quantitative synthesis of previously published research by applying meta-analysis to determine the overall effectiveness of biocontrol in relation to biological and application-oriented factors. For each of 149 entries (antagonist-disease combinations) from 53 reports published in Biological & Cultural Tests between 2000 and 2005, an effect size was calculated as the difference in disease intensity expressed in standard deviation units between the biocontrol treatment and its corresponding untreated control. Effect sizes ranged from -1.15 (i.e., disease strongly enhanced by application of the biocontrol agent) to 4.83 (strong disease suppression by the antagonist) with an overall weighted mean of 0.62, indicating moderate effectiveness on average. There were no significant (P >0.05) differences in effect sizes between entries from studies carried out in the greenhouse versus the field, between those involving soilborne versus aerial diseases, or among those carried out in conditions of low, medium, or high disease pressure (expressed relative to the disease intensity in the untreated control). However, effect sizes were greater on annual than on perennial crops, regardless of whether the analysis was carried out for all entries (P = 0.0268) or for those involving only soilborne diseases (P = 0.0343). Effect sizes were not significantly different for entries utilizing fungal versus bacterial biocontrol agents or for those targeting fungal versus bacterial pathogens. However, entries that used r-selected biological control agents (i.e., those having short generation times and producing large numbers of short-lived offspring) were more effective than those that applied antagonists that were not r-selected (P = 0.0312). Interestingly, effect sizes for entries that used Bacillus spp. as biological control agents were lower than for those that applied other antagonists (P = 0.0046 for all entries and P = 0.0114 for soilborne diseases). When only aerial diseases were considered, mean effect size was greater for entries that received one or two sprays than for those that received more than eight sprays of the biocontrol agent (P = 0.0002). This counterintuitive result may indicate that investigators often attempt unsuccessfully to compensate for anticipated poor performance in antagonist-disease combinations by making more applications.

scholarly journals Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides

2020 ◽  
Author(s):  
Tomasz Oszako ◽  
Dmitry Voitka ◽  
Marcin Stocki ◽  
Natalia Stocka ◽  
Justyna Anna Nowakowska ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Rna Polymerase Ii ◽  
Quercus Robur ◽  
Biocontrol Agent ◽  
Elongation Factor ◽  
Preventive Measure ◽  
Protective Effects ◽  
Trichoderma Asperellum ◽  
Erysiphe Alphitoides ◽  
Forest Nurseries

AbstractThe protective effects of Trichoderma asperellum IZR D-11 as a biocontrol agent against the powdery mildew Erysiphe alphitoides infecting leaves of Quercus robur were evaluated for the first time. A strain of Trichoderma had earlier been isolated in Belarus, and was identified in this study as T. asperellum by sequencing of three genomic markers: internal transcribed spacer, translation elongation factor 1 alpha and RNA polymerase II subunit 2, with over 99.2% identity to corresponding genomic sequences in GenBank. When applied once in the first year just after onset of powdery mildew disease, T asperellum IZR D-11 reduced disease progression and continued to reduce powdery mildew levels during the following three years. Photosynthetic activity as represented by chlorophyll fluorescence measured in oak seedlings was increased in treated plants, and greater assimilate production was also found. The use of this antagonistic fungus increased the total water content in oak leaves suggesting that T. asperellum IZR D-11 can serve as a preventive measure to reduce energy losses in the process of water transpiration. GC-MS analysis detected 49 volatile compounds in the headspace of pure cultures of T. asperellum. Sesquiterpenes represented mainly by daucene, dauca-4(11),8-diene and isodaucene were the largest group of compounds emitted. We speculate that these volatiles from T. asperellum IZR D-11 may be involved in induced resistance in the plant, but further research is needed. The above results suggest that T. asperellum strain IZR D-11 has potential as a biocontrol agent of oak powdery mildew in forest nurseries.

scholarly journals Functional analysis of the class II hydrophobin gene HFB2-6 from the biocontrol agent Trichoderma asperellum ACCC30536

2015 ◽  
Vol 171 ◽  
pp. 8-20 ◽  
Author(s):  
Ying Huang ◽  
Gulijimila Mijiti ◽  
Zhiying Wang ◽  
Wenjing Yu ◽  
Haijuan Fan ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Biocontrol Agent ◽  
Class Ii ◽  
Trichoderma Asperellum

scholarly journals Effects of Soil Solarization and Trichoderma asperellum on Soilborne Inoculum of Phytophthora ramorum and Phytophthora pini in Container Nurseries

Plant Disease ◽  
2016 ◽  
Vol 100 (2) ◽  
pp. 438-443 ◽  
Author(s):  
F. Funahashi ◽  
J. L. Parke
Keyword(s):  
Biocontrol Agent ◽  
Soil Surface ◽  
Field Trials ◽  
Phytophthora Ramorum ◽  
Soil Solarization ◽  
Trichoderma Asperellum ◽  
Promising Technique ◽  
Phytophthora Spp ◽  
Container Nurseries ◽  
San Rafael

Infested container nursery beds are an important source of soilborne Phytophthora spp. for initiating disease through movement with surface water or splashing onto foliage. We investigated the effects of soil solarization, alone or with subsequent amendment with a Trichoderma asperellum biocontrol agent, on the survival of Phytophthora spp. inoculum. In field trials conducted with Phytophthora ramorum in San Rafael, CA and with P. pini in Corvallis, OR, infested rhododendron leaf inoculum was buried at 5, 15, and 30 cm below the soil surface. Solarization for 2 or 4 weeks during summer 2012 eliminated recovery of Phytophthora spp. buried at all depths in California trial 1, at 5 and 15 cm in California trial 2, but only at 5 cm in Oregon. There was no significant reduction of Phytophthora spp. recovery after T. asperellum application. Although the population densities of the introduced T. asperellum at the 5-cm depth were often two- to fourfold higher in solarized compared with nonsolarized plots, they were not significantly different (P = 0.052). Soil solarization appears to be a promising technique for disinfesting the upper layer of soil in container nurseries under certain conditions.

scholarly journals Mode of action of Trichoderma asperellum SKT-1, a biocontrol agent against Gibberella fujikuroi

2007 ◽  
Vol 32 (3) ◽  
pp. 222-228 ◽  
Author(s):  
Satoshi Watanabe ◽  
Kazuo Kumakura ◽  
Norihiko Izawa ◽  
Kozo Nagayama ◽  
Takashi Mitachi ◽  
...  
Keyword(s):  
Mode Of Action ◽  
Biocontrol Agent ◽  
Gibberella Fujikuroi ◽  
Trichoderma Asperellum

scholarly journals Disease suppression in Ganoderma-infected oil palm seedlings treated with Trichoderma harzianum

2008 ◽  
Vol 44 (No. 3) ◽  
pp. 101-107 ◽  
Author(s):  
M.Z. Nur Ain Izzati ◽  
F. Abdullah
Keyword(s):  
Oil Palm ◽  
Large Scale ◽  
Biocontrol Agent ◽  
Severity Index ◽  
Disease Suppression ◽  
Scale Production ◽  
Conidial Suspension ◽  
Disease Severity Index ◽  
Artificial Infection ◽  
Large Scale Production

Disease suppression in <I>Ganoderma</I>-infected oil palm seedlings treated with a conidial suspension of <I>Trichoder-ma harzianum</I> FA 1132 was tested in plant house conditions to determine the effectiveness of the fungus as a biocontrol agent. The highest efficacy of control was achieved by treatment right after artificial infection; the total number of infected plants was reduced to give the lowest disease severity index (DSI) value of 5.0%, compared to the infected and non-treated control that had the highest DSI of 70.0%. After conidia suspension of FA 1132 was applied, the colony forming ability by <I>Trichoderma</I> in the soil was dramatically increased, but decreased after some time. Results of the present study are a useful reference basis for further tests in the field and large scale production trials.

scholarly journals Trichoderma asperellum Strain T34 Used as Biocontrol Agent Against Rhizoctonia solani in Potato Plants

2020 ◽  
Vol 19 (2) ◽  
pp. 89-97
Author(s):  
Edgar Vilcacundo ◽  
Maria Isabel Trillas ◽  
Wilman Carrillo
Keyword(s):  
Rhizoctonia Solani ◽  
Biocontrol Agent ◽  
Trichoderma Asperellum ◽  
Potato Plants

scholarly journals Evaluation of a novel endophytic Pseudomonas lactis strain for control of boxwood blight1

2019 ◽  
Vol 37 (2) ◽  
pp. 39-43 ◽  
Author(s):  
Ping Kong
Keyword(s):  
Biocontrol Agent ◽  
Disease Suppression ◽  
Lead Times ◽  
Bacterial Strains ◽  
Control Efficacy ◽  
Public Gardens ◽  
Buxus Sempervirens ◽  
Private And Public ◽  
Culture Growth ◽  
Blight Disease

Abstract Boxwood blight, caused by Calonectria pseudonaviculata (Cps) (Synonyms: Cylindrocladium buxicola, Cylindrocladium pseudonaviculatum), is a devastating disease which affects boxwood (Buxus) in private and public gardens worldwide. SW, an endophyte identified as a strain of Pseudomonas lactis, was isolated along with seven other bacterial strains from symptom-reversed boxwood leaves infected by Cps. SW had the largest population among the 8 isolates. SW inhibited Cps culture growth similar to three other antagonistic isolates but was the strongest suppressor of Cps conidial germination and germling development. When the cell-free supernatant (CFS) from SW liquid cultures was used to treat boxwood plants 48 h prior to inoculation with Cps, it resulted in a 69.4% disease reduction; suggesting involvement of its metabolites in disease suppression. Boxwood blight control efficacy was further evaluated with resuspended SW cell at 108-9 cfu.ml−1 at different treatment lead times. When SW was applied 2 or 10 days before inoculation with Cps, boxwood blight disease decreased by 72% and 67%, respectively. Further extending the lead time to 20 and 30 days, the efficacy was reduced to 26-27%. However, with its features of moderate control efficacy, high population in plant tissue and safety towards humans, SW shows great potential as a biocontrol agent for boxwood blight. Index words: Biocontrol agent, endophyte, plant pretreatment, Calonectria pseudonaviculata suppression. Species used in this study: Bacterium strain SW (Pseudomonas lactis). Plant species: Buxus sempervirens ‘Justin Brouwers'.

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