Temporal and spatially controllable cell encapsulation using a water-soluble phospholipid polymer with phenylboronic acid moiety

Biomaterials ◽  
2007 ◽  
Vol 28 (10) ◽  
pp. 1770-1777 ◽  
Author(s):  
Tomohiro Konno ◽  
Kazuhiko Ishihara
Keyword(s):  
Phenylboronic Acid ◽  
Cell Encapsulation ◽  
Water Soluble ◽  
Acid Moiety ◽  
Phospholipid Polymer

Control of cell function on a phospholipid polymer having phenylboronic acid moiety

2010 ◽  
Vol 5 (5) ◽  
pp. 054101 ◽  
Author(s):  
Aya Saito ◽  
Tomohiro Konno ◽  
Hiroki Ikake ◽  
Kimio Kurita ◽  
Kazuhiko Ishihara
Keyword(s):  
Cell Function ◽  
Phenylboronic Acid ◽  
Acid Moiety ◽  
Phospholipid Polymer

Bioinspired and Multifunctional Phospholipid Polymer Nanoparticles

2016 ◽  
Vol 102 ◽  
pp. 3-11
Author(s):  
Kazuhiko Ishihara ◽  
Wei Xin Chen ◽  
Yuuki Inoue
Keyword(s):  
Acid Group ◽  
Covalent Bonding ◽  
Water Soluble ◽  
Polymer Nanoparticles ◽  
Butyl Methacrylate ◽  
Carboxylic Acid Group ◽  
Cleavage Reaction ◽  
Phospholipid Polymer ◽  
Biodegradable Poly ◽  
Immobilized Proteins

Photoreactive and cytocompatible polymer nanoparticles for immobilizing and photoinduced releasing proteins were prepared. A water-soluble and amphiphilic phospholipid polymer, poly (2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-4-(4-(1-methacryloyloxyethyl)-2-methoxy-5-nitrophenoxy) butyric acid (PL)) (PMB-PL) was synthesized. The PMB-PL underwent a cleavage reaction at the PL unit by photoirradiation at a wavelength of 365 nm. Additionally, the PMB-PL took polymer aggregate in aqueous medium and was used to modify the surface of biodegradable poly (L-lactic acid) (PLA) nanoparticle as an emulsifier. The morphology of the PMB-PL/PLA nanoparticle was spherical and approximately 130 nm in diameter. The carboxylic acid group in the PL unit could be used for immobilization of proteins by covalent bonding. The bound proteins were released by a photoinduced cleavage reaction. Within 60 sec, up to 90% of the immobilized proteins were released by photoirradiation and activity of the protein released in the medium was maintained as well as that the original proteins before immobilization. Octa-arginine (R8) could promote internalization of the protein/PLA/PMB-PL nanoparticles into cells when the R8 was co-immobilized on the nanoparticles. After that, photoirradiation induced protein release from the nanoparticles and proteins distributed more evenly inside cells. From these results, we concluded that PMB-PL/PLA nanoparticles have the potential to be used as smart carriers to deliver proteins to biological systems, such as the inside of living cells.

scholarly journals Importance of Hydrolysis of Amino Acid Moiety in Water-Soluble Prodrugs of Disodium Cromoglycate for Increased Oral Bioavailability.

1992 ◽  
Vol 15 (7) ◽  
pp. 339-345 ◽  
Author(s):  
Akihisa YOSHIMI ◽  
Hiroyuki HASHIZUME ◽  
Satoshi TAMAKI ◽  
Hirokazu TSUDA ◽  
Fumio FUKATA ◽  
...  
Keyword(s):  
Amino Acid ◽  
Oral Bioavailability ◽  
Water Soluble ◽  
Disodium Cromoglycate ◽  
Acid Moiety ◽  
Amino Acid Moiety ◽  
Hydrolysis Of
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