The influence of proepicardial cells on the osteogenic potential of marrow stromal cells in a three-dimensional tubular scaffold

Biomaterials ◽  
2008 ◽  
Vol 29 (14) ◽  
pp. 2203-2216 ◽  
Author(s):  
Mani T. Valarmathi ◽  
Michael J. Yost ◽  
Richard L. Goodwin ◽  
Jay D. Potts
Keyword(s):  
Stromal Cells ◽  
Three Dimensional ◽  
Marrow Stromal Cells ◽  
Osteogenic Potential ◽  
Tubular Scaffold

Isolation Yield and Osteogenic Potential of Human Bone Marrow Stromal Cells are Maintained Irrespective of Age or Gender

2007 ◽  
Vol 361-363 ◽  
pp. 1149-1152
Author(s):  
Jeong Joon Yoo ◽  
Jeon Hyun Bang ◽  
Kyung Hoi Koo ◽  
Kang Sup Yoon ◽  
Hee Joong Kim
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Mononuclear Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Osteogenic Potential ◽  
Donor Age

The relationships between donor age and gender and initial isolation yield and the osteogenic potentials of human bone marrow stromal cells (hBMSCs) have not been clearly elucidated. The authors investigated whether isolation yields and the osteogenic differentiation potentials of hBMSCs are indeed dependent on donor age or gender. Fresh bone marrow was aspirated from iliac crest of 72 donors (mean age 54.1 years; range, 23-84 years; 39 men and 33 women) undergoing total hip arthroplasty. Numbers of mononuclear cells, numbers of colony forming unit-fibroblasts (CFU-Fs) and alkaline phosphatase (ALP)-positive CFU-Fs, and numbers of BMSCs after isolation culture were not found to be significantly dependent on donor age or gender. Moreover, no significant age- or gender-related differences were observed in terms of the proliferation activities, ALP activities, and calcium contents of BMSCs during in vitro osteogenic differentiation. The data obtained from 72 human donors revealed no significant age- or genderrelated differences among hBMSCs in terms of isolation yields, proliferation activities, and osteogenic potentials.

scholarly journals Solution Mediated Effect of Bioactive Glass in Poly (Lactic-Co-Glycolic Acid)- Bioactive Glass Composites on Osteogenesis of Marrow Stromal Cells

2005 ◽  
Vol 284-286 ◽  
pp. 619-622 ◽  
Author(s):  
Jun Yao ◽  
Shula Radin ◽  
Gwendolen Reilly ◽  
Phoebe S. Leboy ◽  
Paul Ducheyne
Keyword(s):  
Bioactive Glass ◽  
Stromal Cells ◽  
Glycolic Acid ◽  
Three Dimensional ◽  
Osteoblastic Differentiation ◽  
Marrow Stromal Cells ◽  
Dimensional Structure ◽  
Composite Scaffolds ◽  
Glass Composites ◽  
Osteogenic Effect

A previous study demonstrated that the incorporation of bioactive glass (BG) into poly (lactic-co-glycolic acid) (PLGA) can promote the osteoblastic differentiation of marrow stromal cells (MSC) on PLGA by forming a calcium phosphate rich layer on its surface. To further understand the mechanisms underlying the osteogenic effect of PLGA-BG composite scaffolds, we tested whether solution-mediated factors derived from composite scaffolds/hybrids can promote osteogenesis of marrow stromal cells. The dissolution product from PLGA-30%BG scaffold stimulated osteogenesis of MSC, as was confirmed by increased mRNA expression of osteoblastic markers such as osteocalcin (OCN), alkaline phosphatase (ALP), and bone sialoprotein (BSP). The three-dimensional structure of the scaffolds may contribute to the production of cell derived factors which promoted distant MSC differentiation. Thus PLGA-BG composites demonstrates significant potential as a bone replacement material.

scholarly journals Three-Dimensional Engineered Bone from Bone Marrow Stromal Cells and Their Autogenous Extracellular Matrix

2009 ◽  
Vol 15 (1) ◽  
pp. 187-195 ◽  
Author(s):  
Fatima N. Syed-Picard ◽  
Lisa M. Larkin ◽  
Charles M. Shaw ◽  
Ellen M. Arruda
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Three Dimensional ◽  
Marrow Stromal Cells

scholarly journals Effect of Platelet-Rich Plasma on Chondrogenic Differentiation in Three-Dimensional Culture

2014 ◽  
Vol 8 (1) ◽  
pp. 78-84 ◽  
Author(s):  
Steven Elder ◽  
John Thomason
Keyword(s):  
Cell Proliferation ◽  
Stromal Cells ◽  
Chondrogenic Differentiation ◽  
Transforming Growth Factor ◽  
Platelet Rich Plasma ◽  
Three Dimensional ◽  
Cartilage Regeneration ◽  
Alginate Beads ◽  
Marrow Stromal Cells ◽  
Significant Difference

Platelet-rich plasma (PRP) may have the potential to enhance articular cartilage regeneration through release of growth factors including transforming growth factor isoforms. The purpose of this study was to investigate the potential for PRP to stimulate chondrogenic differentiation in three-dimensional PRP hydrogel constructs. Allogenic PRP was prepared using a double centrifugation protocol which resulted in a platelet concentration approximately 250% above baseline. Canine marrow stromal cells were encapsulated at 6.8×106 cells/ml in either 2% sodium alginate or in a 3:1 mixture of freshly prepared PRP and 2% alginate. PRP and alginate beads were cultured in chemically defined chondrogenic medium with and without 10 ng/ml TGF-β3. PRP cultures were additionally supplemented with frozen-thawed PRP. In the absence of TGF-β3, PRP had a mild stimulatory effect on cell proliferation. PRP did not stimulate cell proliferation in the presence of TGF-β3. Cells exposed to TGF-β3 accumulated significantly more GAG/DNA than those which were not, but there was not a statistically significant difference between alginate and PRP. Total collagen content was greater in PRP than in alginate, regardless of TGF-β3. Chondrogenesis in PRP was qualitatively and spatially different than that which occurred in conventional alginate beads and was characterized by isolated centers of intense chondrogenesis. Overall the results demonstrate that PRP alone weakly promotes chondroinduction of marrow stromal cells, and the effect is greatly augmented by TGF-β3.

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