Influence of white-rot fungus Polyporus brumalis BRFM 985 culture conditions on the pretreatment efficiency for anaerobic digestion of wheat straw

2018 ◽  
Vol 110 ◽  
pp. 75-79 ◽  
Author(s):  
Elsa Rouches ◽  
Simeng Zhou ◽  
Michelle Sergent ◽  
Sana Raouche ◽  
Helene Carrere
Keyword(s):  
Anaerobic Digestion ◽  
Wheat Straw ◽  
Culture Conditions ◽  
White Rot ◽  
White Rot Fungus ◽  
Polyporus Brumalis

scholarly journals Integrative visual omics of the white-rot fungus Polyporus brumalis exposes the biotechnological potential of its oxidative enzymes for delignifying raw plant biomass

2018 ◽  
Author(s):  
Shingo Miyauchi ◽  
Anaïs Rancon ◽  
Elodie Drula ◽  
Delphine Chaduli ◽  
Anne Favel ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Lignin Degradation ◽  
Plant Biomass ◽  
White Rot ◽  
Fungal Genome ◽  
Oxidative Enzymes ◽  
White Rot Fungus ◽  
Large Set ◽  
Combining Data ◽  
Polyporus Brumalis

AbstractWhite-rot fungi are wood decayers able to degrade all polymers from lignocellulosic biomass including cellulose, hemicelluloses, and lignin. The white-rot fungus Polyporus brumalis efficiently breaks down lignin and is regarded as having a high potential for the initial treatment of plant biomass in its conversion to bio-energy. We performed integrative multi-omics analyses by combining data from the fungal genome, transcriptomes, and secretomes. We found the fungus possessed an unexpectedly large set of genes coding for enzymes related to lignin degradation, and that these were highly expressed and massively secreted under solid-state fermentation conditions. The examination of interrelated multi-omics patterns revealed the coordinated regulation of lignin-active peroxidases and H2O2-generating enzymes along with the activation of cellular mechanisms for detoxification, which combined to result in the efficient lignin breakdown by the fungus.ImportancePlant biomass conversion for green chemistry and bio-energy is a current challenge for a modern sustainable bioeconomy. The complex polyaromatic lignin polymers in raw biomass feedstocks (i.e. agriculture and forestry by-products) are major obstacles for biomass conversions. From a biotechnological aspect, these compounds could be a potential source of aromatic platform molecules for bio-based polymers. Here we describe the extraordinary ability of Polyporus brumalis for lignin degradation using its enzymatic arsenal to break down wheat straw, a lignocellulosic substrate that is considered as a biomass feedstock worldwide. We observed unusual expansions of gene families coding for; 1) Class II peroxidases involved in lignin degradation; and 2) GMC oxidoreductases/dehydrogenases involved in generating the hydrogen peroxide required for lignin peroxidase activity. Our findings suggested the fungus massively mobilizes this oxidative machinery during growth on wheat straw. Overall, we identified sets of co-regulated enzymes, which could potentially augment the efficiency of biotechnological plant biomass conversions.

scholarly journals Alkali treatment of fungal pretreated wheat straw for bioethanol production

Bioethanol ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
María García-Torreiro ◽  
Miguel Álvarez Pallín ◽  
María López-Abelairas ◽  
Thelmo A. Lu-Chau ◽  
Juan M. Lema
Keyword(s):  
Wheat Straw ◽  
Alkali Treatment ◽  
Alkaline Treatment ◽  
White Rot ◽  
White Rot Fungus ◽  
Process Conditions ◽  
Alkali Pretreatment ◽  
Irpex Lacteus ◽  
C 30 ◽  
Pretreated Wheat Straw

AbstractBioconversion of lignocellulosic materials into ethanol requires an intermediate pretreatment step for conditioning biomass. Sugar yields from wheat straw were previously improved by the addition of a mild alkali pretreatment step before bioconversion by the white-rot fungus Irpex lacteus. In this work, an alternative alkaline treatment, which significantly reduces water consumption, was implemented and optimized. Sugar recovery increased 117% with respect to the previously developed alkaline wash process at optimal process conditions (30°C, 30 minutes and 35.7% (w/w) of NaOH). In order to further reduce operational costs, a system for alkali recycling was implemented. This resulted in the treatment of 150% more wheat straw using the same amount of NaOH. Finally, enzymatic hydrolysis was optimized and resulted in a reduction of enzyme dose of 33%.

PCB’s Biotransformation by a White-Rot Fungus Under Composting and Liquid Culture Conditions

2002 ◽  
pp. 287-297
Author(s):  
G. Ruiz-Aguilar ◽  
J. Fernández-Sánchez ◽  
R. Rodríguez-Vázquez ◽  
H. M. Poggi-Varaldo ◽  
F. Esparza-García ◽  
...  
Keyword(s):  
Liquid Culture ◽  
Culture Conditions ◽  
White Rot ◽  
White Rot Fungus

Alkaline-sulfite chemithermomechanical pulping of Eucalyptus grandis biotreated by Ceriporiopsis subvermispora under varied culture conditions

Holzforschung ◽  
2008 ◽  
Vol 62 (4) ◽  
Author(s):  
Marcos Paulo Vicentim ◽  
André Ferraz
Keyword(s):  
Fiber Length ◽  
Corn Steep Liquor ◽  
Eucalyptus Grandis ◽  
Culture Conditions ◽  
Wood Chips ◽  
White Rot ◽  
White Rot Fungus ◽  
Ceriporiopsis Subvermispora ◽  
Fungal Pretreatment ◽  
Chemithermomechanical Pulping

Abstract The effect of different culture conditions have been evaluated concerning the extracellular enzyme activities of the white-rot fungus Ceriporiopsis subvermispora growing on Eucalyptus grandis wood. The consequence of the varied fungal pretreatment on a subsequent chemithermomechanical pulping (CTMP) was addressed. In all cultures, manganese peroxidase (MnP) and xylanase were the predominant extracellular enzymes. The biopulping efficiency was evaluated based on the amount of fiber bundles obtained after the first fiberizing step and the fibrillation levels of refined pulps. It was found that the MnP levels in the cultures correlated positively with the biopulping benefits. On the other hand, xylanase and total oxalate levels did not vary significantly. Accordingly, it was not possible to determine whether MnP accomplishes the effect alone or depends on synergic action of other extracellular agents. Pulp strength and fiber size distribution were also evaluated. The average fiber length of CTMP pulps prepared from untreated wood chips was 623 μm. Analogous values were observed for most of the biopulps; however, significant amounts of shorter fibers were found in the biopulp prepared from wood chips biotreated in cultures supplemented with glucose plus corn-steep liquor. Despite evidence of reduced average fiber length, biopulps prepared from these wood chips presented the highest improvement in tensile indexes (+28% at 23° Schopper-Riegler).

Dibutyl phthalate biodegradation by the white rot fungus,Polyporus brumalis

2007 ◽  
Vol 97 (6) ◽  
pp. 1516-1522 ◽  
Author(s):  
Soo-Min Lee ◽  
Jae-Won Lee ◽  
Bon-Wook Koo ◽  
Myung-Kil Kim ◽  
Don-Ha Choi ◽  
...  
Keyword(s):  
Dibutyl Phthalate ◽  
White Rot ◽  
White Rot Fungus ◽  
Polyporus Brumalis

Xylanase production by white-rot fungus Pleurotus eryngii grown on optimized culture conditions

2008 ◽  
Vol 136 ◽  
pp. S292
Author(s):  
Simair A. Altaf ◽  
Dahot M. Umar ◽  
Mangio S. Muhammad ◽  
Jian-Jiang Zhong
Keyword(s):  
Culture Conditions ◽  
Pleurotus Eryngii ◽  
White Rot ◽  
White Rot Fungus ◽  
Xylanase Production

Induction of laccase activity in the white rot fungus Pleurotus ostreatus using water polluted with wheat straw extracts

2013 ◽  
Vol 133 ◽  
pp. 142-149 ◽  
Author(s):  
Alejandra Parenti ◽  
Elaia Muguerza ◽  
Amaia Redin Iroz ◽  
Alejandra Omarini ◽  
Enma Conde ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Pleurotus Ostreatus ◽  
Laccase Activity ◽  
White Rot ◽  
White Rot Fungus

scholarly journals Anaerobic digestion of olive mill wastewater

2013 ◽  
Vol 11 (3) ◽  
pp. 364-372
Keyword(s):  
Anaerobic Digestion ◽  
White Rot Fungi ◽  
Olive Mill Wastewater ◽  
The Other ◽  
White Rot ◽  
White Rot Fungus ◽  
Stable Operation ◽  
The Stability ◽  
Solids Removal ◽  
Olive Mill

Anaerobic digestion of olive-mill wastewater (OMW) was carried out in a continuously fed mode bioreactor. The bioreactor was operated at different hydraulic retention times (HRTs), using OMW, either raw or pre-treated with white-rot fungi. Two different kinds of feed were tested in this process, one which was thermally treated and subjected to sedimentation, in order to remove the solids contained and the other without any physicochemical treatment (raw OMW). Thermally pretreated OMW did not allow a stable operation even at an HRT of 30d. Further pretreatment of the OMW with a white-rot fungus for removal of the contained phenolics, allowed a stable operation at an HRT of 30 d. On the other hand, simple dilution of the raw wastewater, without any solids removal, lead to a stable operation at an HRT of 30d and was accompanied by higher production of biogas. The presence of the solids in the OMW proved to be a determining factor for the stability of the process and could be attributed to a possible adsorption on the solids of hydrophobic compounds, such as long-chain fatty acids that are toxic to methanogens.

scholarly journals Enhancing of the utilization of palm leaf hay using Bacillus subtilis and Phanerochaete chrysosporium in the diet of lambs under desert conditions

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Hatem A. Hamdon ◽  
Ahmed E. Kholif ◽  
Gamal B. Mahmoud ◽  
Ali M.A. Khalifa ◽  
Mohamed N.M. Abdel Ati
Keyword(s):  
Bacillus Subtilis ◽  
Growth Performance ◽  
Wheat Straw ◽  
Phanerochaete Chrysosporium ◽  
Carcass Characteristics ◽  
Nutrient Digestibility ◽  
White Rot ◽  
White Rot Fungus ◽  
Blood Metabolites ◽  
Palm Leaf

AbstractThe efficiency of the bacterium Bacillus subtilis and white-rot fungus Phanerochaete chrysosporium on growth performance, blood metabolites, carcass characteristics, meat composition and nutrient digestibility of lambs fed crushed whole palm leaf hay was investigated for 105 d. In Experiment 1 (Growth experiment), twenty-four lambs (29.5 ± 1.25 kg) were divided into four feed-based treatments: (1) concentrate and wheat straw ad libitum (control), (2) palm leaf hay (DPL), (3) palm leaf hay supplemented with 2 g of B. subtilis and P. chrysosporium (DPL2) or (4) palm leaf hay supplemented with 4 g of B. subtilis and P. chrysosporium (DPL4). In Experiment 2 (Digestibility experiment), twelve rams, three from each treatment, were used to study nutrient digestibility using the faecal bag technique. Results of the Experiment 1 showed that the DPL4 showed greater (P=0.049) live-weight gain than the control. The diets containing palm leaf hay showed lower (P=0.001) roughage and total feed intake and higher (P=0.001) feed efficiency than the control. Increased serum total protein, globulin, urea-N, aspartate aminotransferase and alanine aminotransferase (ALT) levels were observed with the DPL4 (P˂0.05). Additionally, diets containing palm leaf hay showed higher ALT levels than the control. The DPL4 increased the hot carcass weight (P=0.006) and dressing percentage (P=0.036), and the diets supplemented with B. subtilis and P. chrysosporium decreased (P˂0.05) the tail fat and all fat levels compared with the DPL treatment. Results of the Experiment 2 showed that nutrient digestibility was lower (P˂0.05) with the DPL and DPL2 than with the control and DPL4. Compared with the control, nutrient digestibility of DPL4 was not affected. It is concluded that replacement of wheat straw with palm leaf hay did not affect the feed utilization or animal performance of the lambs; however, supplementation with B. subtilis and P. chrysosporium at 4 g/lamb daily enhanced the growth performance, nutrient digestibility, and carcass characteristics without any adverse effects on blood metabolites.

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