Treatment with Trichilia catigua ethyl-acetate fraction improves healing and reduces oxidative stress in TNBS-induced colitis in rats

BACKGROUND: Oxidative stress in type 2 diabetes mellitus (T2D) causes insulin resistance and disordered insulin secretion. Pathomechanisms of T2D consist of dysfunctional pancreatic β-cell and insulin resistance caused by free radical (reactive oxygen species and reactive nitrogen species) that produced from the glucose metabolism pathway. Insulin resistance can be measured using the homeostatic model assessment of insulin resistance (HOMA-IR). Oxidative stress can measure through the activities of malondialdehyde (MDA) and superoxide dismutase (SOD). AIM: This research aims to study the potential of chayote (Sechium edule Jacq. Swartz) to be used as antihyperglycemic in T2D. MATERIALS AND METHODS: This research was conducted with a post-test randomized controlled group design. Eleven groups with four male rats each were used. Normal untreated rats were treated under ad libitum feeding and drinking condition. Meanwhile, the rat models were induced with the combination of 45 mg/kg b.w. streptozotocin, 110 mg/kg b.w. nicotinamide, 40.5 mg/kg b.w. metformin, high-fat diet, and/or chayote extract. The chayote extract was orally administered to the rat in the form of ethanol extract and/or ethyl acetate fraction, with three dosages of 45 mg/kg b.w., 100 mg/kg b.w., and 150 mg/kg b.w. for each extract type. The body weight, glucose level, insulin level, MDA, and SOD activities were measured. The HOMA-IR was used. RESULTS: The lowest body weight of the rat model in week 0 was 145 ± 25.31, founded in Group H that was treated with ethyl acetate fraction of chayote extract (45 mg/kg b.w.). The lowest blood sugar level in the group with 2 h glucose load was 112.5 ± 27.00 on average, found in Group G that was treated with chayote ethanolic extract (150 mg/kg b.w.). The highest SOD in the group treated with chayote extract was 1.27 ± 0.20, founded in Group H treated with ethyl acetate 45 mg/kg b.w. The lowest level of MDA was 0.86 ± 0.70 in Group H treated with ethyl acetate 45 mg/kg b.w. The lowest fasting blood sugar spectrophotometer level was 150.54 ± 17.24 mg/dl in Group K with metformin treatment, followed by 155.16 ± 31.92 mg/dl in Group K treated 45 mg/kg b.w. ethanol treatment. The highest insulin level was 6.14 ± 0.71, founded in Group F that was treated with chayote ethanolic extract 100 mg/kg b.w. The lowest measurement of HOMA-IR was 0.16 ± 0.80 in Group E treated with ethanol extract of chayote 45 mg/kg b.w. CONCLUSION: Ethanol extract and fractionation of chayote work as an antioxidant and anti-insulin resistance.

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Ethyl-acetate fraction of Trichilia catigua restores long-term retrograde memory and reduces oxidative stress and inflammation after global cerebral ischemia in rats

Behavioural Brain Research ◽

10.1016/j.bbr.2017.08.050 ◽

2018 ◽

Vol 337 ◽

pp. 173-182 ◽

Cited By ~ 14

Author(s):

Jacqueline Godinho ◽

Rúbia Maria Weffort de Oliveira ◽

Anacharis Babeto de Sa-Nakanishi ◽

Cristiano Correia Bacarin ◽

Claudia Hitomi Huzita ◽

...

Keyword(s):

Oxidative Stress ◽

Cerebral Ischemia ◽

Ethyl Acetate ◽

Ethyl Acetate Fraction ◽

Global Cerebral Ischemia ◽

Retrograde Memory

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Ethyl-acetate fraction of Trichilia catigua protects against oxidative stress and neuroinflammation after cerebral ischemia/reperfusion

Journal of Ethnopharmacology ◽

10.1016/j.jep.2018.04.018 ◽

2018 ◽

Vol 221 ◽

pp. 109-118 ◽

Cited By ~ 9

Author(s):

Jacqueline Godinho ◽

Anacharis Babeto de Sa-Nakanishi ◽

Lucas Stafuza Moreira ◽

Rúbia Maria Weffort de Oliveira ◽

Claudia Hitomi Huzita ◽

...

Keyword(s):

Oxidative Stress ◽

Cerebral Ischemia ◽

Ethyl Acetate ◽

Ischemia Reperfusion ◽

Ethyl Acetate Fraction ◽

Cerebral Ischemia Reperfusion

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Effects of Pine Needle Ethyl Acetate Fraction on Membrane Fluidity and Oxidative Stress in Liver Membranes of Rats

Journal of Life Science ◽

10.5352/jls.2003.13.5.684 ◽

2003 ◽

Vol 13 (5) ◽

pp. 684-691

Keyword(s):

Oxidative Stress ◽

Ethyl Acetate ◽

Membrane Fluidity ◽

Ethyl Acetate Fraction ◽

Pine Needle ◽

Liver Membranes ◽

And Oxidative Stress

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Regulating role of ethyl acetate fraction of Tephrosia tinctoria pers. in carbohydrate metabolism and oxidative stress in diabetic rats

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2019.108842 ◽

2019 ◽

Vol 114 ◽

pp. 108842

Author(s):

Rajaram Krishnasamy ◽

Sureshkumar Periyasamy

Keyword(s):

Oxidative Stress ◽

Ethyl Acetate ◽

Carbohydrate Metabolism ◽

Ethyl Acetate Fraction ◽

Diabetic Rats ◽

And Oxidative Stress

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Ethyl Acetate Fraction of Abelmoschus manihot (L.) Medic Flowers Exerts Inhibitory Effects Against Oxidative Stress in H2O2-Induced HepG2 Cells and D-Galactose-Induced Aging Mice

Journal of Medicinal Food ◽

10.1089/jmf.2021.k.0053 ◽

2021 ◽

Vol 24 (9) ◽

pp. 997-1009

Author(s):

Chang Liu ◽

Yanli Jia ◽

Yan Qiu

Keyword(s):

Oxidative Stress ◽

Ethyl Acetate ◽

Hepg2 Cells ◽

Ethyl Acetate Fraction ◽

Inhibitory Effects

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Ethyl Acetate Fraction of Hemerocallis citrina Baroni Decreases Tert-butyl Hydroperoxide-Induced Oxidative Stress Damage in BRL-3A Cells

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/1526125 ◽

2018 ◽

Vol 2018 ◽

pp. 1-13 ◽

Cited By ~ 2

Author(s):

Jing Wang ◽

Dongmei Hu ◽

Jing Hou ◽

Shushu Li ◽

Weiping Wang ◽

...

Keyword(s):

Oxidative Stress ◽

Ethyl Acetate ◽

Antioxidant Capacity ◽

Ethyl Acetate Fraction ◽

Glutamate Cysteine Ligase ◽

Butyl Hydroperoxide ◽

Tert Butyl Hydroperoxide ◽

Hemerocallis Citrina ◽

Stress Damage

The main purposes of this study were to screen the antioxidant activities of various fractions of Hemerocallis citrina Baroni and test their hepatoprotective effects in vitro. Antioxidant assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and reducing power experiments) and tert-butyl hydroperoxide- (t-BHP-) induced BRL-3A oxidative damage experiments were performed in vitro. The H. citrina ethyl acetate fraction (HCEA) was determined to have strong antioxidant activity because of its high flavonoid and polyphenol content. Ultraperformance liquid chromatography- (UPLC-) photodiode array (PDA)/mass spectrometry (MS) analysis showed that the main components of the HCEA were flavonoids and caffeic acid derivatives. A total of 17 compounds were identified. HCEA also effectively protected the liver against t-BHP-induced oxidative stress injury and significantly reduced reactive oxygen (ROS) accumulation. Moreover, HCEA significantly reduced levels of alanine aminotransferase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH). Further studies have shown that HCEA inhibits t-BHP-induced apoptosis by increasing B-cell lymphoma-2 (BCL-2) activity and decreasing caspase-3 and caspase-9 activity. Moreover, HCEA enhanced the activity of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), as well as the total antioxidant capacity (T-AOC), and increased the antioxidant level of glutathione (GSH) in BRL-3A cells. HCEA increased the antioxidant capacity of cells by increasing the gene expression of AMP-activated protein kinase (AMPK), extracellular signal-regulated kinase (ERK), P38, nuclear factor, erythroid 2 like 2 (Nrf2), SOD, glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM), and heme oxygenase 1 (HO-1), which are associated with antioxidant pathways to protect against oxidative stress. In conclusion, HCEA protected BRL-3A cells against t-BHP-induced oxidative stress damage via antioxidant and antiapoptosis pathways. Therefore, H. citrina Baroni may serve as a potential hepatoprotective drug.

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Croton argenteus preparation inhibits initial growth, mitochondrial respiration and increase the oxidative stress from Senna occidentalis seedlings

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201520140238 ◽

2015 ◽

Vol 87 (2) ◽

pp. 753-763 ◽

Cited By ~ 2

Author(s):

KATLIN S. RECH ◽

CRISTIANE B. SILVA ◽

JULIANA D. KULIK ◽

JOSIANE F.G. DIAS ◽

SANDRA M.W. ZANIN ◽

...

Keyword(s):

Oxidative Stress ◽

Ethyl Acetate ◽

Crude Extract ◽

Ethanolic Extract ◽

Ethyl Acetate Fraction ◽

Initial Growth ◽

Mitochondrial Energy Metabolism ◽

Atp Production ◽

Phytotoxic Activity ◽

Catalase Peroxidase

Senna ocidentalis is a weed, native to Brazil, considered to infest crops and plantations, and is responsible for yield losses of several crops, particularly soybean. The aim of this work was to evaluate if theCroton argenteus extract and fractions possess phytotoxic activity on S. ocidentalis. The crude ethanolic extract (CEE) and its hexanic (HF), chloroformic (CLF) and ethyl acetate (EAF) fractions were tested in germination, growth, oxidative stress increase, Adenosine triphosphate, L-malate and succinate synthesis. The crude extract and its fractions slowed down the germination of S. ocidentalis and decreased the final percentage of germination. Oxidative stress was also increased in the seedlings, by an increase of catalase, peroxidase, superoxide dismutase, glutathione reductase and lipid peroxidation; and it became clear that the ethyl acetate fraction was more phytotoxic. The results indicate that the crude extract and fractions of C. argenteus compromise the mitochondrial energy metabolism, by the inhibition of mitochondrial ATP production, with a decrease in the production of L-malate and succinate. The ethyl acetate fraction of C. argenteus showed high activity on germination and growth, and these effects take place by means of mitochondrial metabolism alterations and increase the oxidative stress, leading the seedling death.

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