scholarly journals Ethyl Acetate Fraction of Hemerocallis citrina Baroni Decreases Tert-butyl Hydroperoxide-Induced Oxidative Stress Damage in BRL-3A Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Jing Wang ◽  
Dongmei Hu ◽  
Jing Hou ◽  
Shushu Li ◽  
Weiping Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Ethyl Acetate Fraction ◽  
Glutamate Cysteine Ligase ◽  
Butyl Hydroperoxide ◽  
Tert Butyl Hydroperoxide ◽  
Hemerocallis Citrina ◽  
Stress Damage

The main purposes of this study were to screen the antioxidant activities of various fractions of Hemerocallis citrina Baroni and test their hepatoprotective effects in vitro. Antioxidant assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and reducing power experiments) and tert-butyl hydroperoxide- (t-BHP-) induced BRL-3A oxidative damage experiments were performed in vitro. The H. citrina ethyl acetate fraction (HCEA) was determined to have strong antioxidant activity because of its high flavonoid and polyphenol content. Ultraperformance liquid chromatography- (UPLC-) photodiode array (PDA)/mass spectrometry (MS) analysis showed that the main components of the HCEA were flavonoids and caffeic acid derivatives. A total of 17 compounds were identified. HCEA also effectively protected the liver against t-BHP-induced oxidative stress injury and significantly reduced reactive oxygen (ROS) accumulation. Moreover, HCEA significantly reduced levels of alanine aminotransferase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH). Further studies have shown that HCEA inhibits t-BHP-induced apoptosis by increasing B-cell lymphoma-2 (BCL-2) activity and decreasing caspase-3 and caspase-9 activity. Moreover, HCEA enhanced the activity of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), as well as the total antioxidant capacity (T-AOC), and increased the antioxidant level of glutathione (GSH) in BRL-3A cells. HCEA increased the antioxidant capacity of cells by increasing the gene expression of AMP-activated protein kinase (AMPK), extracellular signal-regulated kinase (ERK), P38, nuclear factor, erythroid 2 like 2 (Nrf2), SOD, glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM), and heme oxygenase 1 (HO-1), which are associated with antioxidant pathways to protect against oxidative stress. In conclusion, HCEA protected BRL-3A cells against t-BHP-induced oxidative stress damage via antioxidant and antiapoptosis pathways. Therefore, H. citrina Baroni may serve as a potential hepatoprotective drug.

scholarly journals Protective Effect of Natural and Synthetic Anthocyanins against Tert-butyl-hydroperoxide-induced Oxidative Damages in Normal and β-thalassemic Major Human Erythrocytes In Vitro

2020 ◽  
Vol 17 (1) ◽  
pp. 38-47
Author(s):  
Naïma Charif ◽  
Nassima Mokhtari-Soulimane ◽  
Sabri Cherrak ◽  
Hafida Merzouk ◽  
Mourad Elhabiri
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Thalassemia Major ◽  
Dpph Assay ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Oxidative Damages ◽  
Blood Disorder ◽  
Tert Butyl Hydroperoxide

Background: Even though β-thalassemia major is a genetic blood disorder, the damages endured by erythrocytes are mediated in part by oxidative stress. Antioxidants such as anthocyanins are capable to prevent the pro-oxidant effects induced by reactive oxygen species (ROS). Objective: This study aims to evaluate the in vitro preventive effects of one natural and two synthetic anthocyanins on normal and β-thalassemic erythrocytes on which toxicity has been induced by the free radical generator: tert-butyl-hydroperoxide TBHP. Methods: Erythrocytes isolated from fasting blood samples of healthy and β-thalassemic major individuals were treated either with TBHP alone or with TBHP after being pre-incubated with anthocyanins. Cell viability, reduced glutathione (GSH) and malondialdehyde (MDA) contents were measured after 90 minutes of incubation. In parallel, the antiradical scavenging capacities of the investigated anthocyanins were also estimated by using the 2,2-DiPhenyl-1-PicrylHydrazyl (DPPH•) assay. Results: The results clearly demonstrate that the treatment of erythrocytes with TBHP induces hemolysis along with marked redox state alteration (lipid peroxidation concomitant to GSH depletion) in both normal and β-thalassemia erythrocytes. During the pre-treatment with anthocyanins, erythrocytes become more resistant to oxidative impairments. Cyanin chloride and 6,7,3’,4’- tetrahydroxyflavylium chloride effectively prevent from TBHP-induced: hemolysis, lipid peroxidation and GSH depletion in normal and thalassemic erythrocytes, while 3’4’-dihdroxy-7-methoxyflavylium chloride had a lesser effect on MDA levels with thalassemic erythrocytes. These results are in agreement with those derived from the DPPH• assay. Conclusion: Our study contributes with important insights that tested anthocyanins may exert relevant potential in the alleviation of oxidative stress, especially the one affecting β-thalassemia erythrocytes.

scholarly journals Assessment of In-Vitro Antioxidant Potential of Ethyl Acetate Fraction of Hydroalcoholic Extract of Aerva Javanica Linn. Flowering Tops

1970 ◽  
Vol 7 (5) ◽  
pp. 133-136
Author(s):  
Alok Khunteta ◽  
Surendra K Swarnkar ◽  
Manish Kumar Gupta ◽  
Aruna Swarnkar ◽  
Pankaj Jain ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Alcoholic Extract ◽  
Total Antioxidant ◽  
Pharmacological Potential

Aerva javanica (Amaranthaceae) is a grey coloured woolly perennial tomentose shrub. Its traditional and folklore usage motivates further investigation on its pharmacognostic parameters and pharmacological potential. Therefore, in order to establish its antioxidant potential, DPPH, SOD and superoxide scavenging and total antioxidant capacity, were determined. Hydro-alcoholic extract (CE) was prepared from flowering tops of A. javanica. In order to work further on activity guided fractions, ethyl acetate (AJEAF) fraction was prepared.  For in-vitro evaluation, ascorbic acid was used as standard antioxidant compound. In DPPH assay IC50 was determined as 89.00 µg/ml, as compared with standard ascorbic acid with IC50 21.80 µg/ml, with a concentration dependent scavenging of free radical. Superoxide scavenging potential in terms of SOD expressed as IC50, was determined as 61.904 µg /ml for AJEAF in contrast to 132.413 µg /ml for standard ascorbic acid. This was equivalent to 16.154 Eq SOD units /mg (EAF) per mg of sample respectively against 7.552 Eq SOD units /mg of standard. Total antioxidant capacity was found to be 283.67 mg Ascorbic acid Eq /g. Results indicated that fraction (AJEAF had significant antioxidant potential which expressed the prospective potential of fraction against metabolic disorders.  

scholarly journals The Effect of Oxidative Stress on the Chicken Ovary: Involvement of Microbiota and Melatonin Interventions

Antioxidants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1422
Author(s):  
Jianping Wang ◽  
Ru Jia ◽  
Haojie Gong ◽  
Pietro Celi ◽  
Yong Zhuo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ovarian Function ◽  
Stress Factors ◽  
Ovary Cell ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Laying Performance ◽  
Chicken Ovary

The poultry ovary is used as a classic model to study ovarian biology and ovarian cancer. Stress factors induced oxidative stress to cause follicle atresia, which may be a fundamental reason for the reduction in fertility in older laying hens or in aging women. In the present study, we set out to characterize the relationships between oxidative stress and ovarian function. Layers (62 weeks of age; BW = 1.42 ± 0.12 kg) were injected with tert-butyl hydroperoxide (tBHP) at 0 (CON) and 800 μmol/kg BW (oxidative stress group, OS) for 24 days and the role of melatonin (Mel) on tBHP-induced ovary oxidative stress was assessed through ovary culture in vitro. The OS (800 μmol/kg BW tert-butyl hydroperoxide) treatment decreased the reproduction performance and ovarian follicle numbers. OS decreased the expression of SIRT1 and increased the P53 and FoxO1 expression of the ovary. A decreased Firmicutes to Bacteroidetes ratio, enriched Marinifilaceae (family), Odoribacter (genus) and Bacteroides_plebeius (species) were observed in the cecum of the OS group. Using Mel in vitro enhanced the follicle numbers and decreased the ovary cell apoptosis induced by tBHP. In addition, it increased the expression of SIRT1 and decreased the P53 and FoxO1 expression. These findings indicated that oxidative stress could decrease the laying performance, ovarian function and influence gut microbiota and body metabolites in the layer model, while the melatonin exerts an amelioration the ovary oxidative stress through SIRT1-P53/FoxO1 pathway.

scholarly journals In vitro Anti-Inflammatory and Anti-Oxidative Stress Activities of Kushenol C Isolated from the Roots of Sophora flavescens

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1768
Author(s):  
Byoung Ok Cho ◽  
Denis Nchang Che ◽  
Ji-Su Kim ◽  
Jang Hoon Kim ◽  
Jae Young Shin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Diseases ◽  
Hacat Cells ◽  
Sophora Flavescens ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Raw264.7 Macrophages ◽  
Tert Butyl Hydroperoxide ◽  
Anti Inflammatory

Kushenol C (KC) is a prenylated flavonoid isolated from the roots of Sophora flavescens aiton. Little is known about its anti-inflammatory and anti-oxidative stress activities. Here, we investigated the anti-inflammatory and anti-oxidative stress effects of KC in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, and tert-butyl hydroperoxide (tBHP)-induced oxidative stress in HaCaT cells. The results demonstrated that KC dose-dependently suppressed the production of inflammatory mediators, including NO, PGE2, IL-6, IL1β, MCP-1, and IFN-β in LPS-stimulated RAW264.7 macrophages. The study demonstrated that the inhibition of STAT1, STAT6, and NF-κB activations by KC might have been responsible for the inhibition of NO, PGE2, IL-6, IL1β, MCP-1, and IFN-β in the LPS-stimulated RAW264.7 macrophages. KC also upregulated the expression of HO-1 and its activities in the LPS-stimulated RAW264.7 macrophages. The upregulation of Nrf2 transcription activities by KC in the LPS-stimulated RAW264.7 macrophages was demonstrated to be responsible for the upregulation of HO-1 expression and its activity in LPS-stimulated RAW264.7 macrophages. In HaCaT cells, KC prevented DNA damage and cell death by upregulating the endogenous antioxidant defense system involving glutathione, superoxide dismutase, and catalase, which prevented reactive oxygen species production from tert-butyl hydroperoxide (tBHP)-induced oxidative stress in HaCaT cells. The upregulated activation of Nrf2 and Akt in the PI3K-Akt signaling pathway by KC was demonstrated to be responsible for the anti-oxidative stress activity of KC in HaCaT cells. Collectively, the study suggests that KC can be further investigated as a potential anti-inflammatory candidate for the treatment of inflammatory diseases.

scholarly journals Maerua angolensis DC. (Capparaceae) Stem Bark Extract Protects against Pentylenetetrazole-Induced Oxidative Stress and Seizures in Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Charles Kwaku Benneh ◽  
Robert Peter Biney ◽  
Augustine Tandoh ◽  
Felix Agyei Ampadu ◽  
Donatus Wewura Adongo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Fraction ◽  
Stem Bark ◽  
Bark Extract ◽  
Anticonvulsant Effect ◽  
Sprague Dawley

Introduction. The stem bark of Maerua angolensis DC. (Capparaceae) is traditionally used for management of epilepsy. Our aim was to evaluate the antiseizure potential and identify possible mechanisms by which the effects are registered. Methods. The petroleum ether/ethyl acetate extract (100–1000 mg kg−1) was administered per os to male Sprague-Dawley rats after pretreatment with flumazenil (0.3 mg kg−1) or L-arginine (150 mg kg−1) or sildenafil (5 mg kg−1) and they subsequently received a subcutaneous injection of pentylenetetrazole (65 mg kg−1). Rats were observed for latency to and duration of myoclonic seizures and additionally the level of protection against oxidant markers and products was assessed in vitro and in vivo. Results. The extract (300 and 1000 mg kg−1, p.o.) significantly delayed the onset and decreased the duration and frequency of PTZ-induced convulsions. The anticonvulsant effect of MAE (300 mg kg−1, p.o.) was reversed by pretreatment with flumazenil, L-arginine, or sildenafil. Also, MAE (300 mg kg−1) treatment reversed significantly PTZ-induced oxidative stress in rat brain tissue. Conclusion. The petroleum ether/ethyl acetate fraction exhibits antiseizure activity by affecting GABAergic and nitric oxide-cGMP pathways. In addition, the extract protects against the generation of free radicals and the oxidative products of the PTZ-induced seizures.

scholarly journals Phytochemicals of Minthostachys diffusa Epling and Their Health-Promoting Bioactivities

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 144
Author(s):  
Immacolata Faraone ◽  
Daniela Russo ◽  
Lucia Chiummiento ◽  
Eloy Fernandez ◽  
Alka Choudhary ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Mass Spectrometry Analysis ◽  
Antioxidant Power ◽  
Spectrometry Analysis ◽  
In Silico Docking ◽  
Aerial Parts ◽  
Health Promoting

The genus Minthostachys belonging to the Lamiaceae family, and is an important South American mint genus used commonly in folk medicine as an aroma in cooking. The phytochemical-rich samples of the aerial parts of Minthostachys diffusa Epling. were tested for pharmacological and health-promoting bioactivities using in vitro chemical and enzymatic assays. A range of radical scavenging activities of the samples against biological radicals such as nitric oxide and superoxide anion and against synthetic 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals, the ferric reducing antioxidant power and the lipid peroxidation inhibition were determined and ranked using the ‘relative antioxidant capacity index’ (RACI). The ethyl acetate fraction showed the highest RACI of +1.12. Analysis of the various fractions’ inhibitory ability against enzymes involved in diabetes (α-amylase and α-glucosidase), and against enzymes associated with Parkinson’s or Alzheimer’s diseases (acetylcholinesterase and butyrylcholinesterase) also suggested that the ethyl acetate fraction was the most active. Liquid chromatography–tandem mass spectrometry analysis of the ethyl acetate fraction showed more than 30 polyphenolic compounds, including triterpenes. The inhibitory cholinesterase effects of the triterpenes identified from M. diffusa were further analysed by in silico docking of these compounds into 3D-structures of acetylcholinesterase and butyrylcholinesterase. This is the first study on pharmacological activities and phytochemical profiling of the aerial parts of M. diffusa, showing that this plant, normally used as food in South America, is also rich in health-promoting phytochemicals.

scholarly journals Perilla frutescens Leaf Extract and Fractions: Polyphenol Composition, Antioxidant, Enzymes (α-Glucosidase, Acetylcholinesterase, and Tyrosinase) Inhibitory, Anticancer, and Antidiabetic Activities

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 315
Author(s):  
Zhenxing Wang ◽  
Zongcai Tu ◽  
Xing Xie ◽  
Hao Cui ◽  
Kin Weng Kong ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Animal Experiments ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Glycogen Accumulation ◽  
Inhibitory Ability

This study aims to evaluate the bioactive components, in vitro bioactivities, and in vivo hypoglycemic effect of P. frutescens leaf, which is a traditional medicine-food homology plant. P. frutescens methanol crude extract and its fractions (petroleum ether, chloroform, ethyl acetate, n-butanol fractions, and aqueous phase residue) were prepared by ultrasound-enzyme assisted extraction and liquid–liquid extraction. Among the samples, the ethyl acetate fraction possessed the high total phenolic (440.48 μg GAE/mg DE) and flavonoid content (455.22 μg RE/mg DE), the best antioxidant activity (the DPPH radical, ABTS radical, and superoxide anion scavenging activity, and ferric reducing antioxidant power were 1.71, 1.14, 2.40, 1.29, and 2.4 times higher than that of control Vc, respectively), the most powerful α-glucosidase inhibitory ability with the IC50 value of 190.03 μg/mL which was 2.2-folds higher than control acarbose, the strongest proliferative inhibitory ability against MCF-7 and HepG2 cell with the IC50 values of 37.92 and 13.43 μg/mL, which were considerable with control cisplatin, as well as certain inhibition abilities on acetylcholinesterase and tyrosinase. HPLC analysis showed that the luteolin, rosmarinic acid, rutin, and catechin were the dominant components of the ethyl acetate fraction. Animal experiments further demonstrated that the ethyl acetate fraction could significantly decrease the serum glucose level, food, and water intake of streptozotocin-induced diabetic SD rats, increase the body weight, modulate their serum levels of TC, TG, HDL-C, and LDL-C, improve the histopathology and glycogen accumulation in liver and intestinal tissue. Taken together, P. frutescens leaf exhibits excellent hypoglycemic activity in vitro and in vivo, and could be exploited as a source of natural antidiabetic agent.

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