Glucoamylase production by solid-state fermentation using rice flake manufacturing waste products as substrate

2006 ◽  
Vol 97 (10) ◽  
pp. 1161-1166 ◽  
Author(s):  
Hema Anto ◽  
U.B. Trivedi ◽  
K.C. Patel
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Waste Products ◽  
Glucoamylase Production

scholarly journals Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation

2020 ◽  
Vol 2 (4) ◽  
pp. 13-23
Author(s):  
Nabiha Naeem Sheikhs ◽  
Qurat-ul-ain ◽  
Saba Altaf
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Bacterial Strain ◽  
Hydrolytic Enzymes ◽  
Skim Milk ◽  
Cost Effective ◽  
Protease Production ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Waste Products

Proteases (also known as peptidases or proteinases) are hydrolytic enzymes that cleave proteins into amino acids. They comprise 60% of the total industrial usage of enzymes worldwide and can be obtained from many sources. The current study aims to isolate and screen protease-producing bacterial strains from the soil and to produce protease from the bacterial co-cultures using solid-state fermentation (SSF). Primary screening of the protease-producing bacterial strains was carried out on skim milk agar and they were sub-cultured and preserved on the nutrient agar for further testing. Thirty-two compatibility tests of twenty-seven bacterial isolates were performed and SSF was carried out. Afterward, absorbance was taken at 660 nm against tyrosine as standard. According to the results, the bacterial co-culture 19 showed the highest absorbance with an enzyme activity of 10.2 U/ml. The bacterial strains of the co-culture 19 were identified through morphological and biochemical tests. Bacterial strain 1 was observed as cocci and irregular, while bacterial strain 2 was bacillus and rod-shaped. Both strains were positive for gram staining, catalase test, casein hydrolysis test and methyl red test. As for endospore staining, bacterial strain 1 was spore forming while bacterial strain 2 was a non-spore former. It was concluded that the bacterial co-culture 19 can act as a potent co-culture for protease production. Compatibility test was carried out to enhance the production of protease by utilizing cheap and readily available agro-waste products, which benefit the industry by being cost effective and the environment by being eco-friendly.

Optimization of glucoamylase production by Mucor indicus, Mucor hiemalis, and Rhizopus oryzae through solid state fermentation / Mucor indicus, Mucor hiemalis, ve Rhizopus oryzae tarafından üretien glukoamilazın katı hal fermantasyonu ile optimizasyonu

2016 ◽  
Vol 41 (4) ◽  
Author(s):  
Sanaz Behnam ◽  
Keikhosro Karimi ◽  
Morteza Khanahmadi ◽  
Zahra Salimian
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Rhizopus Oryzae ◽  
Industrial Applications ◽  
Mucor Hiemalis ◽  
Suitable Candidate ◽  
Cultivation Time ◽  
Mucor Indicus ◽  
Glucoamylase Production

AbstractObjective: Glucoamylase is a hydrolyzing enzyme with several industrial applications. Glucoamylase was produced via a solid state fermentation by three naturally occurring zygomycetes fungi of Mucor indicus, Mucor hiemalis, and Rhizopus oryzae on wheat bran.Methods: The effects of cultivation temperature, medium moisture content, and cultivation time on the enzyme production were investigated. Experiments were designed with an orthogonal central composite design on the three variables using response surface methodology (RSM).Results: For glucoamylase production, the optimum temperature and medium moisture content for the three fungi were 26.6°C and 71.8%, respectively. The optimum cultivation time for M. hiemalis and R. oryzae was 33.1 h, while it was 66.8 h for M. indicus. At optimum conditions, glucoamylase production by M. indicus, M. hiemalis, and R. oryzae was respectively 255.3, 272.3, and 1545.3 U per g dry substrate.Conclusion: R. oryzae is a suitable candidate for industrial production of glucoamylase.

scholarly journals Influence of Carbon, Nitrogen and Phosphorous Sources on Glucoamylase Production by Aspergillus awamori in Solid State Fermentation

2003 ◽  
Vol 58 (9-10) ◽  
pp. 708-712 ◽  
Author(s):  
Telma Elita Bertolin ◽  
Willibaldo Schmidell ◽  
Alfredo E. Maiorano ◽  
Janice Casara ◽  
Jorge A. V. Costa
Keyword(s):  
Solid State ◽  
Carbon Source ◽  
Solid State Fermentation ◽  
Carbon Sources ◽  
Aspergillus Awamori ◽  
Main Carbon Source ◽  
Nitrogen Phosphorus ◽  
Additional Carbon Source ◽  
Main Carbon ◽  
Glucoamylase Production

AbstractIt was the objective of the present study to increase the production of glucoamylase by Aspergillus awamori through solid state fermentation, using wheat bran as the main carbon source and (NH4)2SO4, urea, KH2PO4, glucose, maltose and starch as additional nitrogen, phosphorus, and carbon sources. The production of glucoamylase is strongly influenced by N and C sources. A 100% increase was observed when the (NH4)2SO4 was replaced by urea, with C/N = 4.8, using maltose as the additional carbon source. C/P ratios in a range of 5.1 to 28.7 did not induce glucoamylase production under the studied conditions.

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