Development of chemically defined media supporting high cell density growth of Ketogulonicigenium vulgare and Bacillus megaterium

2011 ◽  
Vol 102 (7) ◽  
pp. 4807-4814 ◽  
Author(s):  
Jing Zhang ◽  
Jingwen Zhou ◽  
Jie Liu ◽  
Kejie Chen ◽  
Liming Liu ◽  
...  
Keyword(s):  
Cell Density ◽  
Bacillus Megaterium ◽  
High Cell Density ◽  
High Cell ◽  
Chemically Defined Media ◽  
Ketogulonicigenium Vulgare ◽  
Defined Media ◽  
Density Growth

scholarly journals Development of Chemically Defined Media Supporting High-Cell-Density Growth of Lactococci, Enterococci, and Streptococci

2008 ◽  
Vol 75 (4) ◽  
pp. 1080-1087 ◽  
Author(s):  
Guiying Zhang ◽  
David A. Mills ◽  
David E. Block
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Fractional Factorial ◽  
Chemical Components ◽  
Statistical Experimental Design ◽  
High Cell ◽  
Maximum Biomass ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Density Growth

ABSTRACT Lactococcus lactis IL1403 was used as an experimental strain to develop a chemically defined medium for study of the physiology and metabolic pathways of lactococci. An experimental leave-one-out technique was employed to determine the necessity of each of the 57 chemical components used in medium development. A statistical experimental design approach including three fractional factorial designs and a central composite design was used to optimize the fermentation process with 21 variables composed of 19 nutritional factors grouped from the 57 components and two environmental factors (initial pH and temperature). For L. lactis IL1403, the maximum biomass concentrations obtained with the two optimal chemically defined media developed in this study (ZMB1 and ZMB2) were generally 3.5- to 4-fold higher than the maximum biomass concentrations obtained with the previously described best synthetic media (SA) and 50% to 68% higher than the maximum biomass concentrations obtained with M17, a complex medium commonly used for lactococci. The new chemically defined media support high-cell-density growth of numerous strains of L. lactis, Enterococcus faecalis, and Streptococcus thermophilus.

scholarly journals High cell density growth of High Five suspension cells in DO-controlled wave-mixed bioreactors

2011 ◽  
Vol 5 (Suppl 8) ◽  
pp. P123 ◽  
Author(s):  
Teddy Beltrametti ◽  
Nicole C Bögli ◽  
Gerhard Greller ◽  
Regine Eibl ◽  
Dieter Eibl
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
High Cell ◽  
Suspension Cells ◽  
Density Growth

Construction of Halomonas bluephagenesis capable of high cell density growth for efficient PHA production

2018 ◽  
Vol 102 (10) ◽  
pp. 4499-4510 ◽  
Author(s):  
Yilin Ren ◽  
Chen Ling ◽  
Ivan Hajnal ◽  
Qiong Wu ◽  
Guo-Qiang Chen
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
High Cell ◽  
Density Growth ◽  
Pha Production

Effects of mutations in acetate metabolism on high-cell-density growth of Escherichia coli

2000 ◽  
Vol 24 (6) ◽  
pp. 421-430 ◽  
Author(s):  
J Contiero ◽  
C Beatty ◽  
S Kumari ◽  
C L DeSanti ◽  
W R Strohl ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Density ◽  
High Cell Density ◽  
Acetate Metabolism ◽  
High Cell ◽  
Density Growth
Sign in / Sign up

Export Citation Format

Share Document