Optimized simultaneous saccharification and co-fermentation of rice straw for ethanol production by Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture using design of experiments

2013 ◽  
Vol 142 ◽  
pp. 171-178 ◽  
Author(s):  
Nopparat Suriyachai ◽  
Khatiya Weerasaia ◽  
Navadol Laosiripojana ◽  
Verawat Champreda ◽  
Pornkamol Unrean
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Design Of Experiments ◽  
Ethanol Production ◽  
Rice Straw ◽  
Scheffersomyces Stipitis ◽  
Simultaneous Saccharification

Expression of serpins by Clostridium thermocellum and simultaneous saccharification of lignocellulosic biomass to enhance ethanol production

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 8662-8676
Author(s):  
Maria Mushtaq ◽  
Muhammad Javaid Asad ◽  
Muhammad Zeeshan Hyder ◽  
Syed Muhammad Saqlan Naqvi ◽  
Saad Imran Malik ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Wheat Straw ◽  
Corn Stover ◽  
Rice Straw ◽  
Clostridium Thermocellum ◽  
Ethanol Yield ◽  
Biofuel Production ◽  
Yeast Fermentation ◽  
Second Generation Bioethanol ◽  
Simultaneous Saccharification

Utilization of biomass for production of second generation bioethanol was considered as a way to reduce burdens of fossil fuel in Pakistan. The materials wheat straw, rice straw, cotton stalk, corn stover, and peel wastes were used in this experiment. Various parameters, such as acidic and alkali pretreatment, enzymatic hydrolysis by cellulases, and effect of proteases inhibitors on ethanol production, were examined. Fermentation was completed by the yeasts Saccharomyces cerevisiae and Clostridium thermocellum separately, and their ethanol production were compared and maximum ethanol yield was obtained with wheat straw i.e.,11.3 g/L by S. cerevisiae and 8.5 g/L by C. thermocellum. Results indicated that a higher quantity of sugar was obtained from wheat straw (19.6 ± 1.6 g/L) followed by rice straw (17.6 ± 0.6 g/L) and corn stover (16.1 ± 0.9 g/L) compared to the other evaluated biomass samples. A higher yield of ethanol (11.3 g/L) was observed when a glucose concentration of 21.7 g/L was used, for which yeast fermentation efficiency was 92%. Results also revealed the increased in ethanol production (93%) by using celluases in combination with recombinant Serine protease inhibitors from C. thermocellum. It is expected that the use of recombinant serpins with cellulases will play a major role in the biofuel production by using agricultural biomass. This will also help in the economics of the biofuel.

Bioethanol Production from Rice Straw in the MAFF Research Project, Japan

2009 ◽  
Vol 1219 ◽  
Author(s):  
Ken Tokuyasu
Keyword(s):  
Heat Treatment ◽  
Saccharomyces Cerevisiae ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Bioethanol Production ◽  
Chemical Pretreatment ◽  
Simple Method ◽  
Herbaceous Biomass ◽  
Free Glucose ◽  
Simultaneous Saccharification

AbstractRice straw is among the most abundant herbaceous biomass, and regarded as the central feedstock for bioethanol production in Japan. We found that significant amounts of soft carbohydrates (SCs), defined as carbohydrates readily recoverable by mere extraction from the biomass or brief enzymatic saccharification, exist in rice straw in the form of free glucose, free fructose, sucrose, starch, and β-1,3-1,4-glucan. Based on the finding, we proposed a simple method for bioethanol production from rice straw samples with SCs, by a heat treatment for sterilization and starch gelatinization, followed by simultaneous saccharification/fermentation with Saccharomyces cerevisiae. This method would offer an efficient process for bioethanol production without the aid of harsh thermo/chemical pretreatment step.

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