Ferrofluid-assisted rapid and directional harvesting of marine microalgal Chlorella sp. used for biodiesel production

2017 ◽  
Vol 244 ◽  
pp. 1337-1340 ◽  
Author(s):  
Shih-Hsin Ho ◽  
Sheng-Yi Chiu ◽  
Chien-Ya Kao ◽  
Tsai-Yu Chen ◽  
Yu-Bin Chang ◽  
...  
2016 ◽  
Vol 78 (7) ◽  
Author(s):  
Costantine Joannes ◽  
Rachel Fran Mansa ◽  
Suhaimi Md. Yasir ◽  
Jedol Dayou

Lately, research on biodiesel production as a renewable and sustainable energy has become increasingly apparent due to the fact that fossil fuel is decreasing and the concern of global warming issues. The third generation of biofuel, which is microalgae-based biodiesel had gained interest over the last decade. The ability of microalgae to grow in various conditions is one of its advantages as the potential and promising feedstock for biodiesel. Microalgae can be cultivated in three modes such as photoautotrophic, heterotrophic and mixotrophic culture mode. Unlike photoautotrophic mode where light is required, the heterotrophic mode mainly utilized carbon compounds to grow. On the other hand, the mixotrophic mode is the condition where light and carbon compounds are supplied for microalgae culturing. This paper investigates the cell growth of Chlorella sp. cultivated in photoautotrophic, heterotrophic and mixotrophic culture mode. It was found that Chlorella sp. was capable of producing the highest cell concentration of 6.67 ± 0.56 x 106 cell mL-1 in the photoautotrophic mode for 23 days of cultivation period. This was 1.3 times and 3.2 times greater than the cell concentration in mixotrophic (5.02 ± 0.49 x 106 cell mL-1) and heterotrophic (2.03 ± 0.29 x 106 cell mL-1) culture, respectively. On the contrary, the highest specific growth rate obtained in the study was from heterotrophic mode (0.32 ± 0.04 day-1) followed by photoautotrophic and mixotrophic mode with 0.26 ± 0.05 day-1 and 0.20 ± 0.04 day-1, respectively. Chlorella sp. cell grew well under the photoautotrophic and mixotrophic mode. However, the insufficient of glucose level had contributed to lower cells productivity in the heterotrophic culture. Therefore, the mixotrophic mode could also be an alternative pathway in microalgae cultivation for biodiesel production if the glucose supplied was adequate and at the suitable level.  


2018 ◽  
Vol 122 ◽  
pp. 80-88 ◽  
Author(s):  
Richa Katiyar ◽  
Randhir K. Bharti ◽  
B.R. Gurjar ◽  
Amit Kumar ◽  
Shalini Biswas ◽  
...  

2020 ◽  
Vol 141 ◽  
pp. 03009
Author(s):  
Pichayatorn Bunkaew ◽  
Sasithorn Kongruang

The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 0C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 0C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.


2017 ◽  
Vol 76 (3) ◽  
pp. 719-727 ◽  
Author(s):  
Samadhan Yuvraj Bagul ◽  
Randhir K. Bharti ◽  
Dolly Wattal Dhar

Microalgae are reported as the efficient source of renewable biodiesel which should be able to meet the global demand of transport fuels. Present study is focused on assessment of wastewater grown indigenous microalga Chlorella sp. for fuel quality parameters. This was successfully grown in secondary treated waste water diluted with tap water (25% dilution) in glass house. The microalga showed a dry weight of 0.849 g L−1 with lipid content of 27.1% on dry weight basis on 21st day of incubation. After transesterification, the yield of fatty acid methyl ester was 80.64% with major fatty acids as palmitic, linoleic, oleic and linolenic. The physical parameters predicted from empirical equations in the biodiesel showed cetane number as 56.5, iodine value of 75.5 g I2 100 g−1, high heating value 40.1 MJ kg−1, flash point 135 °C, kinematic viscosity 4.05 mm2 s−1 with density of 0.86 g cm3 and cold filter plugging point as 0.7 °C. Fourier transform infra-red (FTIR), 1H, 13C NMR spectrum confirmed the chemical nature of biodiesel. The results indicated that the quality of biodiesel was almost as per the criterion of ASTM standards; hence, wastewater grown Chlorella sp. can be used as a promising strain for biodiesel production.


2013 ◽  
Vol 147 ◽  
pp. 471-476 ◽  
Author(s):  
Naruetsawan Sanyano ◽  
Pakamas Chetpattananondh ◽  
Sininart Chongkhong

2014 ◽  
Vol 37 (10) ◽  
pp. 2083-2094 ◽  
Author(s):  
Ramasamy Praveenkumar ◽  
Bohwa Kim ◽  
Eunji Choi ◽  
Kyubock Lee ◽  
Sunja Cho ◽  
...  

2018 ◽  
Vol 55 (4C) ◽  
pp. 14 ◽  
Author(s):  
Ha Vinh Hung

Microalgae are a promising feedstock for biodiesel production. Harvesting of microalgal biomass is still a bottleneck to its commercial scale application, due to small cell size, low culture densities, colloidal stability and thus economic disadvantage. The aim of this study was to evaluate the biomass separation of the small size microalgae Chlorella sp. by electrochemical flotation process with rectangle electrodes using aluminum or iron plates. The most effective conditions for this experiment involved the use of an aluminum electrode for 30 min with a current density of 1.5 mA/cm2, whereas the iron electrode has been used ineffectively with the same of conditions. The effect of current density (0.5–3 mA/cm2), concentration of microalgae biomass (0.29–1.5 g/L), and electrolyte (0–2 g/L) for aluminum electrode were analyzed. The highest recovery efficiency of 90 % was obtained for Chlorella sp. at 1.5 mA/cm2 in 30 min and concentration of microalgae biomass of 0.74 - 1.5 g/L with power consumption of 1.36 kWh/kg.  The electrochemical flotation process with aluminum electrodes could be a possible harvesting step at commercial scale for microalgal biomass production.


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