Enhanced reducing sugar production by saccharification of lignocellulosic biomass, Pennisetum species through cellulase from a newly isolated Aspergillus fumigatus

Abstract BackgroundLignocellulosic biomass for biofuel production was considered as an effective way to develop new energy. However, the efficient sugar conversion of cellulose and practical utilization of lignin are great challenges for sustainable biorefinery. In addition, sugar conversion and lignin utilization are generally performed separately. In this study, high reducing sugar production and multiple lignin nanoparticles preparation were realized in a pattern based on tetrahydrofuran-water (THF-H2O) pretreatment of corn straw (CS). ResultsThe maximum production of the reducing sugar was 26.79 g/l, which was significantly higher than the theoretical yield of 20.65 g/l. Lignin nanoparticles with different sizes ranged from 239 to 798 nm were prepared using dissolved lignin in the supernatant fluid from different THF-H2O pretreatment conditions through self-assembly with introducing water. The formation of lignin particles with different sizes were influenced by soluble lignin characteristics in the pretreatment liquid. The lignin molecular, functional groups, and structure were explored to elucidate the effects on the variation of lignin particles sizes by GPC, FTIR, and 2D-HSQC-NMR. The guaiacyl (G)-type lignin was easier to be dissolved in the mild pretreatment liquid, contributing to form smaller lignin nanoparticles with a good dispersibility. Comparatively, a small content of syringyl- and G-type lignin which caused by the lignin depolymerization retained in the severe pretreatment liquid to form the larger sphere particles. ConclusionsThe optimal pretreatment under TH22 (THF-H2O pretreatment at 120 °C for 2 h) simultaneously realized the utilization of all components in biomass through high reducing sugar production and the smaller lignin particles preparation. This new pattern of CS pretreatment plays a novel perspective for the technical design of lignocellulosic biomass conversion.

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Pretreatment and Enzymatic Hydrolysis of Lignocellulosic Biomass for Reducing Sugar Production

Applied Environmental Science and Engineering for a Sustainable Future - Valorisation of Agro-industrial Residues – Volume II: Non-Biological Approaches ◽

10.1007/978-3-030-39208-6_1 ◽

2020 ◽

pp. 1-27

Author(s):

Noor Idayu Nashiruddin ◽

Nor Hasmaliana Abdul Manas ◽

Roshanida A. Rahman ◽

Nur Izyan Wan Azelee ◽

Daniel Joe Dailin ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Lignocellulosic Biomass ◽

Reducing Sugar ◽

Sugar Production ◽

Hydrolysis Of

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Green preparation of covalently co-immobilized multienzymes on silica nanoparticles for clean production of reducing sugar from lignocellulosic biomass

Journal of Cleaner Production ◽

10.1016/j.jclepro.2021.127994 ◽

2021 ◽

pp. 127994

Author(s):

Yuanqing Lin ◽

Wenhui Jin ◽

Lixi Cai ◽

Xin Liu ◽

Yue Qiu ◽

...

Keyword(s):

Silica Nanoparticles ◽

Lignocellulosic Biomass ◽

Reducing Sugar ◽

Clean Production

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Peningkatan Produksi Gula Pereduksi dari Tandan Kosong Kelapa Sawit dengan Praperlakuan Asam Organik pada Reaktor Bertekanan

REAKTOR ◽

10.14710/reaktor.16.4.199-206 ◽

2017 ◽

Vol 16 (4) ◽

pp. 199

Author(s):

Fahriya Puspita Sari ◽

Nissa Nurfajrin Solihat ◽

Sita Heris Anita ◽

Fitria Fitria ◽

Euis Hermiati

Keyword(s):

Enzymatic Hydrolysis ◽

Oxalic Acid ◽

Organic Acid ◽

Oil Palm ◽

Maleic Acid ◽

Reducing Sugar ◽

Sugar Production ◽

Empty Fruit Bunch ◽

Acid Pretreatment ◽

Severity Factor

ENHANCEMENT OF REDUCING SUGAR PRODUCTION FROM OIL PALM EMPTY FRUIT BUNCH BY PRETREATMENT USING ORGANIC ACID IN PRESSURIZED REACTOR. Organic acids are potential to create more environmentally friendly process in the pretreatment of lignocellulosic biomass for bioethanol production. This study was aimed to investigate the influence of organic acid pretreatment in reducing sugar production in a pressurized reactor with various resident times and temperatures on enzymatic hydrolysis of OPEFB. Two different organic acids (maleic acid and oxalic acid) were used in the pretreatment of oil palm empty fruit bunch (OPEFB) using a pressurized reactor. Factorial design using three different temperatures (170, 180, and 190°C) and four resident times (15, 30, 45, and 60 min) were employed, followed by enzymatic hydrolysis. Each condition conducted two repetitions. Analysis was conducted on the reducing sugar that was produced after saccharification by means of the severity factor of each pretreatment condition. Maleic acid showed higher reducing sugar yield with lower severity factor than oxalic acid with the same operating conditions. The highest yield of reducing sugars (80.84%) was obtained using maleic acid at 170 for 60 minutes with severity factor of 1.836. Keywords: bioethanol; organic acid pretreatment; pressurized reactor; severity factor; oil palm empty fruit bunches; Abstrak Asam organik berpotensi dalam membantu proses praperlakuan dari biomassa lignoselulosa untuk memproduksi bioetanol yang ramah lingkungan. Penelitian ini bertujuan untuk mengetahui pengaruh asam organik, suhu dan waktu operasi terhadap produksi gula pereduksi dengan reaktor bertekanan pada tandan kosong kelapa sawit. Dua asam organik yang berbeda yaitu asam oksalat dan asam maleat digunakan untuk proses praperlakuan tandan kosong kelapa sawit (TKKS) dengan bantuan reaktor bertekanan. Dalam proses praperlakuan digunakan tiga suhu yang berbeda yaitu suhu 170, 180, dan 190°C dan empat waktu operasi 15, 30, 45, dan 60 min yang dilanjutkan dengan proses hidrolisis enzimatis. Setiap kondisi dilakukan dua kali pengulangan. Analisa yang digunakan adalah analisa uji gula pereduksi dan severity factor pada kondisi tiap praperlakuan. Asam maleat menunjukkan hasil yang lebih baik dengan severity factor yang lebih rendah dibandingkan menggunakan asam oksalat dengan kondisi operasi yang sama. Hasil yang didapatkan menunjukkan bahwa praperlakuan tandan kosong kelapa sawit dengan bantuan reaktor bertekanan memiliki rendemen gula pereduksi optimum sebesar 80,84% dengan menggunakan asam maleat pada suhu 170°C selama 60 menit dengan severity factor sebesar 1,836. Kata kunci: bioetanol; praperlakuan asam organik; reaktor bertekanan; severity factor; tandan kosong kelapa sawit.

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PRETREATMENT OF MILLET HUSK USING ALKALINE HYDROGEN PEROXIDE TO ENHANCE ENZYMATIC HYDROLYSIS FOR REDUCING SUGAR PRODUCTION

FUDMA Journal of Sciences ◽

10.33003/fjs-2021-0502-617 ◽

2021 ◽

Vol 5 (2) ◽

pp. 289-294

Author(s):

Zeenat Ibrahim Saulawa ◽

Lawal Nura ◽

Muntari Bala ◽

Abdullahi A. Iman

Keyword(s):

Hydrogen Peroxide ◽

Enzymatic Hydrolysis ◽

Reducing Sugars ◽

Reducing Sugar ◽

Sugar Concentration ◽

Sugar Production ◽

Alkaline Hydrogen Peroxide ◽

Pretreatment Time ◽

Pretreatment Temperature ◽

Reducing Sugar Concentration

The effectiveness of alkaline hydrogen peroxide as a suitable choice of pretreatment for the conversion of millet husk to reducing sugars using cellulase enzyme for hydrolysis and subsequent ethanol production was determined. The effects of three variables on reducing sugar production from millet husk were determined using one factor at a time (OFAT) method namely; peroxide concentration, pretreatment time and pretreatment temperature. From the results, it was observed that a significant (P<0.05) amount of reducing sugars were lost during pretreatment of millet husk. The untreated group which was only physically pretreated (milled) however yielded a significantly higher (P<0.05) reducing sugar concentration of 10.67mg/ml after enzymatic hydrolysis while the highest reducing sugar concentration of 4.82mg/ml was obtained using 0.375%v/v peroxide concentration for 60minutes at 250C. Therefore, pretreatment of biomass with alkaline hydrogen peroxide may be more suitable for feedstock with high lignin contents than millet husk.

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Reducing sugar production from spent coffee grounds using microbubble-assisted synthesis of silica acid catalyst

Catalysis Today ◽

10.1016/j.cattod.2021.11.014 ◽

2021 ◽

Author(s):

SungHo Lee ◽

Lusha Qin ◽

Oi Lun Li

Keyword(s):

Acid Catalyst ◽

Reducing Sugar ◽

Sugar Production ◽

Spent Coffee Grounds ◽

Coffee Grounds

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Derived high reducing sugar and lignin colloid particles from corn stover

BMC Chemistry ◽

10.1186/s13065-020-00725-y ◽

2020 ◽

Vol 14 (1) ◽

Author(s):

Wei Liu ◽

Shengnan Zhuo ◽

Mengying Si ◽

Mengting Yuan ◽

Yan Shi

Keyword(s):

Corn Stover ◽

Lignocellulosic Biomass ◽

Alternative Energy ◽

Reducing Sugar ◽

Sugar Yield ◽

Potential Candidate ◽

Practical Utilization ◽

Lignin Concentration ◽

Colloid Particles ◽

G Ratio

AbstractLignocellulosic biomass is considered as the largest potential candidate to develop alternative energy, such as biofuel, biomaterial. However, the efficient conversion of cellulose and practical utilization of lignin are great challenges for sustainable biorefinery. In this study, high reducing sugar yield and different size of lignin colloid particles (LCPs) were obtained via tetrahydrofuran–water (THF–H2O) pretreatment of corn stover (CS). THF–H2O as a co-solvent, could efficiently dissolve lignin and retain cellulose. After the pretreatment, 640.87 mg/g of reducing sugar was produced, that was 6.66-fold higher than that of the untreated CS. Meanwhile, the pretreatment liquor could form spherical LCPs with different sizes ranged from 202 to 732 nm through self-assembly. We studied the optimal pretreatment condition to simultaneously realize the high reducing sugar yield (588.4 mg/g) and excellent LCPs preparation with average size of 243 nm was under TH22 (THF–H2O pretreatment at 120 °C for 2 h). To further explore the formation of LCPs with different sizes. We studied the lignin structure changes of various conditions, concluded the size of LCPs was related to the lignin concentration and syringyl/guaiacyl (S/G) ratio. As the increase of the lignin concentration and S/G, the sizes of LCPs were increased. G-type lignin was easier to dissolve in the mild pretreatment supernatant, contributing to form smaller LCPs with a good dispersibility. In the severe condition, both of S and G-type lignin were dissolved due to the lignin depolymerization, formed the larger sphere particles. This work provides a novel perspective for the technical design of lignocellulosic biomass conversion.

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