PRETREATMENT OF MILLET HUSK USING ALKALINE HYDROGEN PEROXIDE TO ENHANCE ENZYMATIC HYDROLYSIS FOR REDUCING SUGAR PRODUCTION

2021 ◽  
Vol 5 (2) ◽  
pp. 289-294
Author(s):  
Zeenat Ibrahim Saulawa ◽  
Lawal Nura ◽  
Muntari Bala ◽  
Abdullahi A. Iman
Keyword(s):  
Hydrogen Peroxide ◽  
Enzymatic Hydrolysis ◽  
Reducing Sugars ◽  
Reducing Sugar ◽  
Sugar Concentration ◽  
Sugar Production ◽  
Alkaline Hydrogen Peroxide ◽  
Pretreatment Time ◽  
Pretreatment Temperature ◽  
Reducing Sugar Concentration

The effectiveness of alkaline hydrogen peroxide as a suitable choice of pretreatment for the conversion of millet husk to reducing sugars using cellulase enzyme for hydrolysis and subsequent ethanol production was determined. The effects of three variables on reducing sugar production from millet husk were determined using one factor at a time (OFAT) method namely; peroxide concentration, pretreatment time and pretreatment temperature. From the results, it was observed that a significant (P<0.05) amount of reducing sugars were lost during pretreatment of millet husk. The untreated group which was only physically pretreated (milled) however yielded a significantly higher (P<0.05) reducing sugar concentration of 10.67mg/ml after enzymatic hydrolysis while the highest reducing sugar concentration of 4.82mg/ml was obtained using 0.375%v/v peroxide concentration for 60minutes at 250C. Therefore, pretreatment of biomass with alkaline hydrogen peroxide may be more suitable for feedstock with high lignin contents than millet husk.

IRRIGATION, FERTILIZER, HARVEST DATES AND STORAGE EFFECTS ON THE REDUCING SUGAR AND FRUCTOSE CONCENTRATIONS OF JERUSALEM ARTICHOKE TUBERS

1977 ◽  
Vol 57 (2) ◽  
pp. 591-596 ◽  
Author(s):  
D. G. DORRELL ◽  
B. B. CHUBEY
Keyword(s):  
Management Practices ◽  
Reducing Sugars ◽  
Tuber Yield ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Jerusalem Artichoke ◽  
Reducing Sugar ◽  
Sugar Concentration ◽  
Variable Effect ◽  
Reducing Sugar Concentration

Several management practices were imposed on four Jerusalem artichoke (Helianthus tuberosus L.) accessions to determine changes in tuber yield, sugar content and the percentage of fructose and glucose in the hydrolyzed soluble sugar extracts. The fructose concentration in the reducing sugars declined from 82.3 to 74.2% from 6 September to 24 October. Storage of tubers at 2 C for up to 11 wk reduced the mean level of fructose from 78.2 to 68.0% and had a variable effect on reducing sugar concentration. Supplemental irrigation lowered the reducing sugar concentration significantly, while fertilization had no affect on any quality parameters. The Morden accession, M6, produced the best combination of high tuber yield (37.4 t/ha), reducing sugar concentration (16.3%) and fructose concentration in the reducing sugars (77.4%). Although a long period of growth was desirable to produce maximum yields of both tubers and total sugar, it was accompanied by a reduction in the ratio of fructose to glucose. This crop does not appear to require a high level of management to produce a good quality carbohydrate product.

scholarly journals Chemical Pretreatments on Residual Cocoa Pod Shell Biomass for Bioethanol Production

Bionatura ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 1490-1500
Author(s):  
Jose F. Alvarez-Barreto ◽  
Fernando Larrea ◽  
Maria C. Pinos C ◽  
Jose Benalcázar ◽  
Daniela Oña ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Reducing Sugars ◽  
Ethanol Yield ◽  
Lignin Content ◽  
Reducing Sugar ◽  
Sugar Concentration ◽  
Biomass Composition ◽  
Future Research ◽  
Bioethanol Production ◽  
Acid Pretreatment

Cocoa pod shell is an essential agricultural residue in Ecuador, and this study addressed its potential valorization for bioethanol production. For this, three types of pretreatments, acid, alkaline, and autohydrolysis, were applied to pod shells from two different cocoa types, national and CCN-51. to remove the lignin. Untreated and treated biomasses were characterized by composition, thermal stability, Fourier transformed infrared spectroscopy (FITR), and scanning electron microscopy (SEM). The treated biomass was then enzymatically hydrolyzed with cellulase. Reducing sugars were quantified after pretreatments and enzymatic hydrolysis, and the pretreatment liquors and the enzymatic hydrolysates were subjected to alcoholic fermentation with Saccharomyces cerevisiae. There were substantial differences in composition between both biomasses, particularly in lignin content, with national cocoa having the lowest values. All pretreatment conditions had significant effects on biomass composition, structure, and thermal properties. After alkaline pretreatment, the biomass presented the highest cellulose and lowest lignin contents, resulting in the highest reducing sugar concentration in the pretreatment liquor. The highest lignin content was found after the acid pretreatment, which resulted in low, reducing sugar concentrations. Autohydrolysis produced similar results as the acid pretreatment; however, it resulted in the highest sugar concentration after enzymatic hydrolysis, while the acid-treated sample had negligible levels. After fermentation, there were no differences in productivity among the pretreatment liquors, but autohydrolysis had the largest ethanol yield. In the hydrolysates, it was also autohydrolysis that resulted in higher productivity and yield. Thus, there is an indication of the formation of inhibitors, both enzymatic activity and ethanol production, in the acid and alkaline pretreatments, and this should be tackled in future research. Nonetheless, given the crucial changes observed in biomass, we believe that cocoa pod shell pretreatment has potential for the generation of reducing sugars that could be further used in different bioprocesses, nor only bioethanol production.

scholarly journals Peningkatan Produksi Gula Pereduksi dari Tandan Kosong Kelapa Sawit dengan Praperlakuan Asam Organik pada Reaktor Bertekanan

REAKTOR ◽  
2017 ◽  
Vol 16 (4) ◽  
pp. 199
Author(s):  
Fahriya Puspita Sari ◽  
Nissa Nurfajrin Solihat ◽  
Sita Heris Anita ◽  
Fitria Fitria ◽  
Euis Hermiati
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oxalic Acid ◽  
Organic Acid ◽  
Oil Palm ◽  
Maleic Acid ◽  
Reducing Sugar ◽  
Sugar Production ◽  
Empty Fruit Bunch ◽  
Acid Pretreatment ◽  
Severity Factor

ENHANCEMENT OF REDUCING SUGAR PRODUCTION FROM OIL PALM EMPTY FRUIT BUNCH BY PRETREATMENT USING ORGANIC ACID IN PRESSURIZED REACTOR. Organic acids are potential to create more environmentally friendly process in the pretreatment of lignocellulosic biomass for bioethanol production. This study was aimed to investigate the influence of organic acid pretreatment in reducing sugar production in a pressurized reactor with various resident times and temperatures on enzymatic hydrolysis of OPEFB. Two different organic acids (maleic acid and oxalic acid) were used in the pretreatment of oil palm empty fruit bunch (OPEFB) using a pressurized reactor. Factorial design using three different temperatures (170, 180, and 190°C) and four resident times (15, 30, 45, and 60 min) were employed, followed by enzymatic hydrolysis. Each condition conducted two repetitions. Analysis was conducted on the reducing sugar that was produced after saccharification by means of the severity factor of each pretreatment condition. Maleic acid showed higher reducing sugar yield with lower severity factor than oxalic acid with the same operating conditions. The highest yield of reducing sugars (80.84%) was obtained using maleic acid at 170 for 60 minutes with severity factor of 1.836. Keywords: bioethanol; organic acid pretreatment; pressurized reactor; severity factor; oil palm empty fruit bunches;   Abstrak Asam organik berpotensi dalam membantu proses praperlakuan dari biomassa lignoselulosa untuk memproduksi bioetanol yang ramah lingkungan. Penelitian ini bertujuan untuk mengetahui pengaruh asam organik, suhu dan waktu operasi terhadap produksi gula pereduksi dengan reaktor bertekanan pada tandan kosong kelapa sawit. Dua asam organik yang berbeda yaitu asam oksalat dan asam maleat digunakan untuk proses praperlakuan tandan kosong kelapa sawit (TKKS) dengan bantuan reaktor bertekanan. Dalam proses praperlakuan digunakan tiga suhu yang berbeda yaitu suhu 170, 180, dan 190°C dan empat waktu operasi 15, 30, 45, dan 60 min yang dilanjutkan dengan proses hidrolisis enzimatis. Setiap kondisi dilakukan dua kali pengulangan. Analisa yang digunakan adalah analisa uji gula pereduksi dan severity factor pada kondisi tiap praperlakuan. Asam maleat menunjukkan hasil yang lebih baik dengan severity factor yang lebih rendah dibandingkan menggunakan asam oksalat dengan kondisi operasi yang sama. Hasil yang didapatkan menunjukkan bahwa praperlakuan tandan kosong kelapa sawit dengan bantuan reaktor bertekanan memiliki rendemen gula pereduksi optimum sebesar 80,84% dengan menggunakan asam maleat pada suhu 170°C selama 60 menit dengan severity factor sebesar 1,836. Kata kunci: bioetanol; praperlakuan asam organik; reaktor bertekanan; severity factor; tandan kosong kelapa sawit.

Alkaline hydrogen peroxide pretreatment, enzymatic hydrolysis and fermentation of sugarcane bagasse to ethanol

Fuel ◽  
2014 ◽  
Vol 136 ◽  
pp. 349-357 ◽  
Author(s):  
Sarita C. Rabelo ◽  
Rafael R. Andrade ◽  
Rubens Maciel Filho ◽  
Aline C. Costa
Keyword(s):  
Hydrogen Peroxide ◽  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Alkaline Hydrogen Peroxide

Investigation of Alkaline Hydrogen Peroxide in Aqueous Organic Solvent to Enhance Enzymatic Hydrolysis of Rice Straw

2020 ◽  
Author(s):  
Jaruwan Damaurai ◽  
Thanchanok Preechakun ◽  
Marisa Raita ◽  
Verawat Champreda ◽  
Navadol Laosiripojana
Keyword(s):  
Hydrogen Peroxide ◽  
Enzymatic Hydrolysis ◽  
Organic Solvent ◽  
Rice Straw ◽  
Alkaline Hydrogen Peroxide ◽  
Hydrolysis Of

Ethanol production from enzymatic hydrolysis of sugarcane bagasse pretreated with lime and alkaline hydrogen peroxide

2011 ◽  
Vol 35 (7) ◽  
pp. 2600-2607 ◽  
Author(s):  
S.C. Rabelo ◽  
N.A. Amezquita Fonseca ◽  
R.R. Andrade ◽  
R. Maciel Filho ◽  
A.C. Costa
Keyword(s):  
Hydrogen Peroxide ◽  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Alkaline Hydrogen Peroxide ◽  
Hydrolysis Of

scholarly journals Bioethanol Production from Enzymatic Hydrolyzates of Pretreated Flaxseed Meals by Baker’s Yeast

2021 ◽  
Author(s):  
SAHELI GHOSAL ◽  
JAYATI BHOWAL
Keyword(s):  
Enzymatic Hydrolysis ◽  
Fermentation Process ◽  
Reducing Sugars ◽  
Reducing Sugar ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Bioethanol Production ◽  
Dilute Acid ◽  
Cellulose Conversion ◽  
Flaxseed Meal

Abstract The present study investigated the usefulness of flaxseed meals as a novel feedstock for the production of bioethanol. The proximate composition of the flaxseed meal was carried out before the pretreatment of the flaxseed meal. In this study, flaxseed meal was pretreated with dilute acid, alkali, and aqueous for disruption of lignocellulosic compounds. The acid pretreated flaxseed meal was used for enzymatic hydrolysis by different enzymes (cellulase, α-amylase, and cellulase combined with α-amylase) for better release of reducing sugar. The cellulose conversion to reducing sugar was significantly higher for acid pretreated flaxseed meals. After enzymatic hydrolysis with cellulase, cellulose conversions to reducing sugars were found to be significantly higher than those of α-amylase and cellulase combined with α-amylase. The bioethanol production was also investigated. The fermentation process was carried out by using baker’s yeast (Saccharomyces cerevisiae) with the acid pretreated flaxseed meal enzymatic hydrolyzate. Maximum ethanol production (0.11 g/l) was achieved from the fermented medium obtained from the acid pretreated flaxseed meal followed by enzymatic hydrolysis by using cellulase enzyme. The structural analysis of bioethanol was also investigated by FTIR.

scholarly journals Waktu Optimum Hidrolisis Pati Limbah Hasil Olahan Ubi Kayu (Manihot esculenta Crantz var. Lahumbu) Menjadi Gula Cair Menggunakan Enzim α-Amilase Dan Glukoamilase

2018 ◽  
Vol 5 (2) ◽  
pp. 86-95
Author(s):  
Ardiansyah Ardiansyah ◽  
Nurlansi Nurlansi ◽  
Rustam Musta
Keyword(s):  
Manihot Esculenta ◽  
Reducing Sugar ◽  
Sugar Concentration ◽  
Optimum Time ◽  
Manihot Esculenta Crantz ◽  
A Value ◽  
Time Required ◽  
Reducing Sugar Concentration

This study aims to determine optimum time of action of the enzyme α-amylase and glucoamylase needed in hydrolyze of starch from waste processed cassava (Manihot esculenta Crantz var. Lahumbu). This research was conducted through three main stages, namely the gelatinization, liquefaction and saccharification. The method was used method are liquefaction and  saccharification. The variation time of the stage liquefaction: 12; 24; 36; 48; 60; and 72 minutes and the saccharification stage are: 9; 18; 27; 36; 45; 54; and 63 hours. The results showed that the optimum time required for stage liquefaction using α-amylase enzyme is 48 minutes on the condition of a temperature of 80 oC with a value of 0.09% amylose levels were measured using UV-Vis spectrophotometer. The optimum time required for saccharification step using a glucoamylase which is 54 hours on the conditions of a temperature of 50oC with the amount of reducing sugar concentration of 9.186 g/L as measured using a UV-Vis spectrophotometer.

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