Effect of stirring on growth and cellulolytic enzymes production by Trichoderma harzianum in a novel bench-scale solid-state fermentation bioreactor

ABSTRACT: Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.

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Optimization of productions of cellulolytic enzymes by Aspergillus niger using residue of mango a substrate

Ciência Rural ◽

10.1590/s0103-84782011005000145 ◽

2011 ◽

Vol 41 (12) ◽

pp. 2210-2216 ◽

Cited By ~ 18

Author(s):

Tamires Carvalho dos Santos ◽

Ingrid Souza Cavalcanti ◽

Renata Cristina Ferreira Bonomo ◽

Nivio Batista Santana ◽

Marcelo Franco

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Water Activity ◽

Solid State Fermentation ◽

Cellulolytic Enzymes ◽

Fermentation Time ◽

Kinetic Activity ◽

Activity Of Enzymes ◽

Activity Variable ◽

Statistical Results

The present paper analyses the effects of water activity (0.88, 0.94 and 0.97) and of fermentation time (24, 48, 72, 96 and 120 hours) on the kinetic activity of enzymes cellulolytic, produced during the solid state fermentation of waste from the improvement of mango, with the aid of fungus species Aspergillus niger. Solid state fermentation was carried out at 35°C inside a bacteriological incubator. The statistical results indicated that the best activity for enzyme CMCase was 7.26U g-1 after 74.51 hours of fermentation, whereas for enzyme FPase was 2.55U g-1 after 98.52 hours, both presenting best results in approximately 0.928 of water activity. Pareto charts have showed that fermentation time has greater effect over the activity of enzyme CMCase, while the water activity variable has greater effect over enzyme FPase activity. During fermentation the fungus synthesized the enzymes without the need of inductors other than mango residue and water.

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Application of response surface methodology for producing cellulolytic enzymes by solid-state fermentation from the puple mombin (Spondias purpurea L.) residue

Food Science and Biotechnology ◽

10.1007/s10068-013-0001-4 ◽

2013 ◽

Vol 22 (1) ◽

pp. 1-7 ◽

Cited By ~ 16

Author(s):

Tamires Carvalho dos Santos ◽

George Abreu Filho ◽

Ana Carolina Oliveira ◽

Thiago José Onório Rocha ◽

Fabiano de Paula Pereira Machado ◽

...

Keyword(s):

Response Surface Methodology ◽

Solid State ◽

Response Surface ◽

Solid State Fermentation ◽

Cellulolytic Enzymes ◽

Spondias Purpurea

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Cellulolytic enzymes on lignocellulosic substrates in solid state fermentation by Aspergillus niger

Indian Journal of Microbiology ◽

10.1007/s12088-007-0059-x ◽

2007 ◽

Vol 47 (4) ◽

pp. 323-328 ◽

Cited By ~ 55

Author(s):

M. Subhosh Chandra ◽

Buddolla Viswanath ◽

B. Rajasekhar Reddy

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Solid State Fermentation ◽

Cellulolytic Enzymes ◽

Lignocellulosic Substrates

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Enzyme production by soil filamentous fungi under solid-state fermentation in banana stalk

Comunicata Scientiae ◽

10.14295/cs.v8i3.1817 ◽

2018 ◽

Vol 8 (3) ◽

pp. 424-431 ◽

Cited By ~ 1

Author(s):

Fernanda Castro Pires dos Santos ◽

Joice Raísa Barbosa Cunha ◽

Fábia Giovana Do val de Assis ◽

Patrícia Lopes Leal

Keyword(s):

Solid State ◽

Environmental Factors ◽

Solid State Fermentation ◽

Substrate Concentration ◽

Enzyme Production ◽

Agricultural Waste ◽

Cellulolytic Enzymes ◽

Sustainable Solutions ◽

Research Challenge ◽

Penicillium Spp

The adequate disposal of agricultural waste is one of the major concerns of public officials and a research challenge to obtain sustainable solutions to the problem. In this sense, the objective of this study was to evaluate the banana leaf stalk use as substrate in solid-state fermentation (SSF) for production of amylolytic and cellulolytic enzymes by Penicillium spp. LEMI A11 strain grown under different substrate concentrations, pH and temperature. Effects of different pH conditions (5.0 and 6.0), temperature (30 and 35 °C) and substrate concentration 70 and 90% (in relation to the final volume) of the fermentation were evaluated over 120 hours of fermentation. The results indicated that Penicillium spp. LEMI A11 was able to use the banana stalk as substrate under SSF. The maximum activities for amylase dextraining, amylase saccharifying and CMCase were 0.18; 0.13 and 04 U.g-1, respectively. The effect of environmental factors related to the substrate concentration was significant for saccharifying amylase and CMCase activity only. The interaction between the environmental factors tested was significant for the dextrinizing amylase activity only. It was verified enzyme activity reduction after 96 hours of fermentation for all enzymes. It concluded that banana stalk is an alternative carbon source to be used in SSF for enzyme production by of Penicillium spp. LEMI A11.

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