scholarly journals Enhancement of co-production of nutritional protein and carotenoids in Dunaliella salina using a two-phase cultivation assisted by nitrogen level and light intensity

2019 ◽  
Vol 287 ◽  
pp. 121398 ◽  
Author(s):  
Yixing Sui ◽  
Maarten Muys ◽  
Dedmer B. Van de Waal ◽  
Sarah D'Adamo ◽  
Pieter Vermeir ◽  
...  
Keyword(s):  
Light Intensity ◽  
Dunaliella Salina ◽  
Nitrogen Level ◽  
Two Phase

scholarly journals Planar droplet sizing: Application to a spray of Jet A1 kerosene

2017 ◽  
Author(s):  
Pierre Doublet ◽  
Christine Lempereur ◽  
Virginel Bodoc ◽  
Mikael Orain ◽  
Pierre Gajan
Keyword(s):  
Light Intensity ◽  
Scattered Light ◽  
Droplet Surface ◽  
Scattered Light Intensity ◽  
Field Of View ◽  
Fluorescence Signal ◽  
Droplet Volume ◽  
Image Processing Algorithm ◽  
Two Phase ◽  
Scattering Angle

Optical techniques are  widely employed for their non-intrusive behavior and are applied to two-phase flowinvestigations. Until now, the most commonly used technique to determine the droplet size is the Phase Doppler Anemogranulometry, although it is time consuming for an overall injector characterization. An imaging technique called Planar Droplet Sizing has been used to offer an alternative and provide a spatially-resolved 2D map of the Sauter Mean Diameter (SMD). The measurement is based on the ratio between laser-induced fluorescence and scattered light intensities which are assumed to be proportional respectively to the droplet volume and droplet surface area. However, previous studies revealed that the dependence of fluorescence intensity on the droplet volume can be altered by the absorption of light in the liquid. The scattered light intensity depends on the scattering angle and intensity variations within the field of view must be avoided.The aim of this study is to make the PDS technique operational for a Jet A-1 kerosene spray. A strong absorption of liquid kerosene appears under UV excitation at 266 nm making the technique unsuitable. Under visible excitation at 532 nm, a fluorescent tracer (Pyrromethene 597) must be added to the kerosene to enhance the fluorescence signal. To prevent scattered light intensity variations within the field of view, an optimal scattering angle close to 115° is required. An image processing algorithm is proposed in order to reduce the effects ofmultiple scattering.DOI: http://dx.doi.org/10.4995/ILASS2017.2017.4698

Photosynthesis and Pigments Influenced By Light Intensity and Salinity in the HalophileDunaliella Salina(Chlorophyta)

1982 ◽  
Vol 62 (3) ◽  
pp. 493-508 ◽  
Author(s):  
Laurel A. Loeblich
Keyword(s):  
Light Intensity ◽  
Dunaliella Salina ◽  
Entire Body ◽  
Definition Of

Dunal (1837) originally described the taxon now known asDunaliella salina(Dunal) Teodoresco, 1905, as consisting of spherical or elliptical cells, red-orange to rust coloured. Teodoresco, in his definition of the species (1905) stated that the zoospores had a green chromatophore but in aged zoospores the entire body, including chromatophore, was red. Thus, by definition, any isolate ofDunaliellawhich is not capable of turning redorange to rust coloured should not be consideredD. salina. Several strains have been reported in the literature under the nameD. salinabut do not meet the aforementioned criteria. These include UTEX 200 (= Cambridge 19/3), Mil'ko's strain (Mil'ko, 1963a, b) and Strain No. 6 used by Mironyuk (Drokova, 1970; Drokova, Popova & Tupik, 1964; Drokova & Popova, 1973, 1974; Mironyuk 1969a, b; Mironyuk & Einor, 1968).

scholarly journals MORPHOLOGICAL AND MORPHOMETRICAL FEATURES IN DUNALIELLA SALINA (CHLAMYDOMONADALES, DUNALIELLACEAE) DURING THE TWO-PHASE CULTIVATION MODE

2019 ◽  
Vol 22 ◽  
pp. 157-165
Author(s):  
Andrei B. Borovkov ◽  
Irina N. Gudvilovich ◽  
Olga A. Memetshaeva ◽  
Anna L. Avsiyan ◽  
Alexander S. Lelekov ◽  
...  
Keyword(s):  
Cell Volume ◽  
Dunaliella Salina ◽  
Linear Growth ◽  
Cell Number ◽  
Two Phase ◽  
Cell Content ◽  
Crimean Peninsula ◽  
Cell Parameters ◽  
Maximum Productivity ◽  
Salina Cell

The paper presents studies of morphological and morphometrical characteristics of green halophilic carotenogenic microalga Dunaliella salina (Dunal) Teodoresco, 1905, from the south-west region of Crimean Peninsula. D. salina was cultivated in two-phase mode under conditions of natural illumination at the premises of A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS (IBSS), Sevastopol, Russia. The maximum D. salina cell density was 1.69·106 cell/ml in the “green” phase and 0.84·106 cell/ml in the “red” growth phase. The maximum productivity by cell number reached 0.15 ∙ 106 cell/(ml·day)in the “green” phase while it was lower by 73% in the “red” phase (0.04∙106 cell/(ml·day). Along with the maximum productivity, linear growth stage in the first phase was characterized by a maximum fraction of small (up to 500 μm3 in volume) cells (about 15-29%) and a decrease in cell volume by 40-45% as compared with initial value. The mean of D. salina cell volume in the “red” phase was 30% higher than in the “green” phase. At the same time, the large cell fraction in the “red” phase was consistently high (15-35%). The patterns of change in morphological and morphometrical cell parameters were in accordance to stage and conditions of growth. Thus, cell elongation was noted in the stage of linear growth, while under unfavorable conditions at growth-declining stage cells became more round-shaped, with orange and tile-red coloration and granulation of cell content. It was shown that morphological and morphometric cell parameters can serve as additional criteria for assessment of physiological condition in D. salina culture. The experiment demonstrated the prospects for two-stage D. salina cultivation in Crimea.

scholarly journals Production Characteristics of Dunaliella salina at Two-phase Pilot Cultivation (Crimea)

2020 ◽  
Vol 20 (5) ◽  
Author(s):  
Andrey B. Borovkov ◽  
Irina N. Gudvilovich ◽  
Anna L. Avsiyan ◽  
Nakhimov Olga A. Memetshaeva ◽  
Alexander S. Lelekov ◽  
...  
Keyword(s):  
Dunaliella Salina ◽  
Two Phase ◽  
Production Characteristics
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