Enhanced nitrogen removal through marine anammox bacteria (MAB) treating nitrogen-rich saline wastewater with Fe(III) addition: Nitrogen shock loading and community structure

Nitrogen removal characteristics and the comparison of the microbial community structure were investigated in different anaerobic ammonia oxidation (Anammox) reactors: an anaerobic sequencing batch reactor (ASBR) and a biofilter reactor. The Anammox systems were inoculated with sludge from the second settling tank of a wastewater treatment plant in Guangzhou, China. After successful start up of Anammox, the microbial community structure of different Anammox reactors were studied through high-throughput sequencing. The results showed that anaerobic ammonium oxidation in the ASBR reactor could successfully start up after 134 days, while Anammox in the biofilter could start up after 114 days. In both systems, total nitrogen removal was at 80% after more than 200 days of operation. The diversity of denitrifying microorganisms was high in both reactors, with Planctomycetes as the main taxa. Anammox bacteria belonging to the genera Candidatus Anammoxoglobus and Kuenenia, were dominant in the ASBR, while all three genera of Candidatus, Anammoxoglobus, Kuenenia, and Brocadia, could be detected in the biofilter reactor. Therefore, the biofilter starts up faster than the ASBR, and contains richer species, which makes it more suitable to domesticate Anammox bacteria.

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Unraveling nitrogen removal and microbial response of marine anammox bacteria-dominated consortia to Mo(VI) addition in nitrogen-laden saline wastewater treatment

Separation and Purification Technology ◽

10.1016/j.seppur.2021.118771 ◽

2021 ◽

pp. 118771

Author(s):

Xiuqin Chen ◽

Jin Li ◽

Yulong Zhang ◽

Wenzong Liu

Keyword(s):

Wastewater Treatment ◽

Nitrogen Removal ◽

Anammox Bacteria ◽

Saline Wastewater ◽

Microbial Response

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Screening and Influencing Factors of Halophilic Denitrifying Bacteria

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.1455 ◽

2012 ◽

Vol 550-553 ◽

pp. 1455-1459

Author(s):

Jing Tang ◽

Hong Ming E ◽

Jin Xiang Fu ◽

Jin Nan Chen ◽

Ming Fan

Keyword(s):

Nitrogen Removal ◽

Morphological Characteristics ◽

Development Trend ◽

Denitrifying Bacteria ◽

High Salt ◽

Research Progress ◽

Saline Wastewater ◽

Carbon And Nitrogen ◽

Petroleum Chemical ◽

Seafood Processing

With nitrite or nitrate nitrogen as electron acceptor in the high salt conditons, halophilic denitrifying bacteria can transfer nitrite or nitrate to nitrogen, thereby purifying the high-salt wastewater. Halophilic denitrifying bacteria play an important role in the carbon and nitrogen removal of saline wastewater, such as petroleum, chemical industry, seafood processing and seafood farming. This article dissussed halophilic denitrifying bacteria screening, the main types and the corresponding morphological characteristics, then we focused on the research progress of main factors of halophilic denitrifying bacteria’s growth and nitrogen removal. Finally put forward the current problems of the research and development trend of halophilic denitrifying bacteria.

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Temperature modulates stress response in anammox reactors

10.1101/2020.02.17.952358 ◽

2020 ◽

Author(s):

Robert Niederdorfer ◽

Damian Hausherr ◽

Alejandro Palomo ◽

Jing Wei ◽

Paul Magyar ◽

...

Keyword(s):

Stress Response ◽

Dissolved Oxygen ◽

Nitrogen Removal ◽

High Stress ◽

Anammox Bacteria ◽

Transcriptional Responses ◽

Microbial Biofilms ◽

Temperature Regimes ◽

Anammox Activity ◽

Stress Susceptibility

AbstractAutotrophic nitrogen removal by anaerobic ammonium oxidizing (anammox) bacteria is an energy-efficient nitrogen removal process in wastewater treatment. However, full-scale deployment under mainstream conditions remains challenging for practitioners due to the high stress susceptibility of anammox bacteria towards fluctuations in dissolved oxygen and temperature. Here, we investigated the response of microbial biofilms with verified anammox activity to oxygen shocks under favorable and cold temperature regimes. Genome-centric metagenomics and metatranscriptomics were used to investigate the stress response on various biological levels. We show that temperature regime and strength of oxygen perturbations induced divergent responses from the process level down to the transcriptional profile of individual taxa. Temperature induced distinct transcriptional states in compositionally identical communities and transient pulses of dissolved oxygen resulted in the upregulation of stress-response only under favorable temperatures. Anammox species and other key biofilm taxa display different transcriptional responses to the induced stress regimes.

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Enhanced Nitrogen Removal from Domestic Wastewater by Partial-Denitrification/Anammox in an Anoxic/Oxic Biofilm Reactor

Processes ◽

10.3390/pr10010109 ◽

2022 ◽

Vol 10 (1) ◽

pp. 109

Author(s):

Yu Huang ◽

Yongzhen Peng ◽

Donghui Huang ◽

Jiarui Fan ◽

Rui Du

Keyword(s):

Nitrogen Removal ◽

Oxygen Demand ◽

Domestic Wastewater ◽

Biofilm Reactor ◽

Anammox Bacteria ◽

Stable Operation ◽

Ammonium Oxidation ◽

N Accumulation ◽

Carbon Demand ◽

Oxidation Efficiency

A partial-denitrification coupling with anaerobic ammonium oxidation (anammox) process (PD/A) in a continuous-flow anoxic/oxic (A/O) biofilm reactor was developed to treat carbon-limited domestic wastewater (ammonia (NH4+-N) of 55 mg/L and chemical oxygen demand (COD) of 148 mg/L in average) for about 200 days operation. Satisfactory NH4+-N oxidation efficiency above 95% was achieved with rapid biofilm formation in the aerobic zone. Notably, nitrite (NO2−-N) accumulation was observed in the anoxic zone, mainly due to the insufficient electron donor for complete nitrate (NO3−-N) reduction. The nitrate-to-nitrite transformation ratio (NTR) achieved was as high as 64.4%. After the inoculation of anammox-enriched sludge to anoxic zones, total nitrogen (TN) removal was significantly improved from 37.3% to 78.0%. Anammox bacteria were effectively retained in anoxic biofilm utilizing NO2−-N produced via the PD approach and NH4+-N in domestic wastewater, with the relative abundance of 5.83% for stable operation. Anammox pathway contributed to TN removal by a high level of 38%. Overall, this study provided a promising method for mainstream nitrogen removal with low energy consumption and organic carbon demand.

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