Novel fluorescent sensing system for α-fructosyl amino acids based on engineered fructosyl amino acid binding protein

2007 ◽  
Vol 22 (9-10) ◽  
pp. 1933-1938 ◽  
Author(s):  
Akane Sakaguchi ◽  
Stefano Ferri ◽  
Wakako Tsugawa ◽  
Koji Sode
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Binding Protein ◽  
Fluorescent Sensing ◽  
Acid Binding Protein ◽  
Sensing System ◽  
Amino Acid Binding

scholarly journals Primordial emergence of a nucleic acid-binding protein via phase separation and statistical ornithine-to-arginine conversion

2020 ◽  
Vol 117 (27) ◽  
pp. 15731-15739 ◽  
Author(s):  
Liam M. Longo ◽  
Dragana Despotović ◽  
Orit Weil-Ktorza ◽  
Matthew J. Walker ◽  
Jagoda Jabłońska ◽  
...  
Keyword(s):  
Amino Acids ◽  
Phase Separation ◽  
Amino Acid ◽  
Nucleic Acid ◽  
Binding Protein ◽  
Basic Amino Acid ◽  
Nucleic Acid Binding ◽  
Evolutionary Trajectory ◽  
Nucleic Acid Binding Protein ◽  
Acid Binding Protein

De novo emergence demands a transition from disordered polypeptides into structured proteins with well-defined functions. However, can polypeptides confer functions of evolutionary relevance, and how might such polypeptides evolve into modern proteins? The earliest proteins present an even greater challenge, as they were likely based on abiotic, spontaneously synthesized amino acids. Here we asked whether a primordial function, such as nucleic acid binding, could emerge with ornithine, a basic amino acid that forms abiotically yet is absent in modern-day proteins. We combined ancestral sequence reconstruction and empiric deconstruction to unravel a gradual evolutionary trajectory leading from a polypeptide to a ubiquitous nucleic acid-binding protein. Intermediates along this trajectory comprise sequence-duplicated functional proteins built from 10 amino acid types, with ornithine as the only basic amino acid. Ornithine side chains were further modified into arginine by an abiotic chemical reaction, improving both structure and function. Along this trajectory, function evolved from phase separation with RNA (coacervates) to avid and specific double-stranded DNA binding. Our results suggest that phase-separating polypeptides may have been an evolutionary resource for the emergence of early proteins, and that ornithine, together with its postsynthesis modification to arginine, could have been the earliest basic amino acids.

Revision of the amino acid sequence of human heart fatty acid-binding protein

1990 ◽  
pp. 127-133
Author(s):  
Torsten Börchers ◽  
Peter Højrup ◽  
Søren U. Nielsen ◽  
Peter Roepstorff ◽  
Friedrich Spener ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Human Heart ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Isolation and Characterization of a Type I Gene Encoding a Light-harvesting Chlorophyll a/b-binding Protein of Photosystem II in Peach

1998 ◽  
Vol 123 (4) ◽  
pp. 493-499 ◽  
Author(s):  
Kyu H. Chung ◽  
Dennis E. Buetow ◽  
Schuyler S. Korban
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Photosystem Ii ◽  
Amino Acid Sequence ◽  
Woody Plants ◽  
Light Harvesting ◽  
Binding Protein ◽  
Transit Peptide ◽  
Type I ◽  
Polyadenylation Sites

A nuclear gene, Lhcb1*Pp1, encoding a light-harvesting chlorophyll a/b-binding protein of photosystem II has been isolated from peach [Prunus persica (L.) Batsch. `Stark Earliglo'] leaf genomic DNA, cloned, and sequenced. This gene encodes a precursor polypeptide of 267 amino acids with a transit peptide of 34 and a type I mature protein of 233 amino acids. The amino acid sequence of the mature polypeptide is 89% to 94% and 80% to 94% similar to those encoded by type I Lhcb genes of annual and other woody plants, respectively. In contrast, the amino acid sequence of the peach transit peptide is less conserved being 47% to 69% similar to those of annual plants and only 17% to 22% similar to those of other woody plants. The peach gene was used as a probe for Lhcb gene expression. Lhcb mRNA is detected in leaves of field-grown trees during June to October. Lhcb mRNA is detected at a high level in leaves of peach shoots grown in tissue culture in the light, but only at a trace level in leaves grown in the dark. Some Lhcb genes appear to be light-modulated in stems. Lhcb1*Ppl contains four potential polyadenylation sites. S1 nuclease analysis detected transcripts of the sizes expected from each of the four polyadenylation sites. All four are found in leaves of light-grown shoots and of field-grown trees throughout the growing season. In contrast, only three are detected in stems of light-grown shoots.

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