Permeabilized cells of flavocytochrome b2 over-producing recombinant yeast Hansenula polymorpha as biological recognition element in amperometric lactate biosensors

Membrane fabrication and integration with microfluidic devices has received increased attention for applications including bio-detection (a device providing analytical information in a selective and quantitative manner using a biological recognition element), membrane-based separation, and biological sample purification. The main challenges associated with these applications have been: 1) meeting sensitivity/selectivity requirements, 2) decreasing costs, 3) maintaining the mechanical stability of the membrane, 4) offering high throughput. Therefore, the main goal of this study was to demonstrate size-based membrane separation and bio-detection using double layer channel developed in our lab and to show how the membrane integrated channel can selectively separate rod shape cell separation with various aspect ratio based on size and enhance bio detection rate with flow. Based on an existing double-channel and cross-flow microfluidics platform, we explored various polymeric materials for fabricating porous membranes to use in pore-size-dependent separation. We induced pores via stop-flow lithography, and investigated membrane properties and limitations for pore-size-dependent separation. We investigated potential applications of poly(ethylene glycol) diacrylate (PEGDA)-based membrane integrated platforms in biological molecule detection based on streptavidin and biotin interaction. We demonstrated that flow and concentrations can enhance target detection in this platform.

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Development of membrane in microfluidic device for sorting and detection for potential heterogeneity investigation

10.32920/ryerson.14657574 ◽

2021 ◽

Author(s):

Dehi Joung

Keyword(s):

Pore Size ◽

Mechanical Stability ◽

Membrane Separation ◽

Cross Flow ◽

Polymeric Materials ◽

Membrane Properties ◽

Glycol Diacrylate ◽

Recognition Element ◽

Size Dependent ◽

Biological Recognition

Membrane fabrication and integration with microfluidic devices has received increased attention for applications including bio-detection (a device providing analytical information in a selective and quantitative manner using a biological recognition element), membrane-based separation, and biological sample purification. The main challenges associated with these applications have been: 1) meeting sensitivity/selectivity requirements, 2) decreasing costs, 3) maintaining the mechanical stability of the membrane, 4) offering high throughput. Therefore, the main goal of this study was to demonstrate size-based membrane separation and bio-detection using double layer channel developed in our lab and to show how the membrane integrated channel can selectively separate rod shape cell separation with various aspect ratio based on size and enhance bio detection rate with flow. Based on an existing double-channel and cross-flow microfluidics platform, we explored various polymeric materials for fabricating porous membranes to use in pore-size-dependent separation. We induced pores via stop-flow lithography, and investigated membrane properties and limitations for pore-size-dependent separation. We investigated potential applications of poly(ethylene glycol) diacrylate (PEGDA)-based membrane integrated platforms in biological molecule detection based on streptavidin and biotin interaction. We demonstrated that flow and concentrations can enhance target detection in this platform.

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Surface plasmon resonance and its application to biomedical research

Medicina ◽

10.3390/medicina43050044 ◽

2007 ◽

Vol 43 (5) ◽

pp. 355 ◽

Cited By ~ 7

Author(s):

Asta Kaušaitė ◽

Almira Ramanavičienė ◽

Viktoras Mostovojus ◽

Arūnas Ramanavičius

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Biomedical Research ◽

Plasmon Resonance ◽

Active Substance ◽

Biologically Active ◽

Biologically Active Substance ◽

Recognition Element ◽

Similar Chemical ◽

Biological Recognition

In the recent years, surface plasmon resonance (SPR) has become one of the major methods for studying and determination of biologically active materials exhibiting affinity interactions. SRP biosensors are increasingly used in biochemistry and bioanalytical chemistry to determine antibody-antigen interactions, to investigate DNA hybridization, to diagnose bacteria- and virus-induced diseases, to identify hormones, steroids, and immunoglobulins, to investigate blood plasma coagulation. Using SPR biosensors, it is possible to analyze the mixtures of substances with a very similar chemical structure because SPR allows identifying only those analytes that specifically interact with biologically active substance immobilized on the surface of SPR biosensor. SPR biosensors are applied to monitor interactions between immobilized biologically active substance and analyte in real-time without labeling. On the other hand, it is possible to investigate not only association of analyte with immobilized material, but also the dissociation of a newly formed complex. SPR biosensors in many cases may be used to perform up to 50 measurements with the same SPR chip with an immobilized biological recognition element. Therefore, at present SPR is one of the most promising methods for determining the interactions between ligand and receptor, antigen and antibody, thus being increasingly used in diagnostics and biomedical research.

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Bioelectrochemical detection of L-lactate respiration using genetically modified Hansenula polymorpha yeast cells overexpressing flavocytochrome b2

Bioelectrochemistry ◽

10.1016/j.bioelechem.2009.04.002 ◽

2009 ◽

Vol 76 (1-2) ◽

pp. 175-179 ◽

Cited By ~ 15

Author(s):

Halyna Shkil ◽

Leonard Stoica ◽

Kostyantyn Dmytruk ◽

Oleh Smutok ◽

Mykhailo Gonchar ◽

...

Keyword(s):

Genetically Modified ◽

Hansenula Polymorpha ◽

Yeast Cells ◽

Flavocytochrome B2

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An Electrokinetically Controlled DNA Hybridization Chip

ASME 2nd International Conference on Microchannels and Minichannels ◽

10.1115/icmm2004-2431 ◽

2004 ◽

Author(s):

David Erickson ◽

Xuezhu Liu ◽

Ulrich Krull ◽

Dongqing Li

Keyword(s):

Dna Hybridization ◽

Mass Transfer Rate ◽

Channel Structure ◽

Nucleic Acid Hybridization ◽

Recognition Element ◽

Delivery Technique ◽

Biological Recognition ◽

Long Time ◽

On Line ◽

Time Required

Biosensors and more specifically biochips exploit the interactions between a target analyte and an immobilized biological recognition element to produce a measurable signal. Systems based on surface phase nucleic acid hybridization, such as modern microarrays, are particularly attractive due to the high degree of selectivity in the binding interactions. One drawback of this reaction is the relatively long time required for complete hybridization to occur, as a result of the diffusion limited reaction kinetics. In this work an electrokinetically controlled DNA hybridization microfluidic chip will be introduced. The electrokinetic delivery technique provides the ability to dispense controlled sample sizes to the hybridization array while serving to increase the mass transfer rate and therefore the reaction speed. The focus of this paper will be on the design and microfabrication of the chip, the unique H-type channel structure and electrokinetic sample delivery and washing technique, and development of the on-line hybridization scanning. Initial hybridization results presented here demonstrate that less than 5 minutes and 4.9nL of 0.5μM ssDNA sample was required (35s dispensing period followed by a 4 minute wash) for complete hybridization.

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Biological Recognition Element

IUPAC Standards Online ◽

10.1515/iupac.90.0292 ◽

2019 ◽

Author(s):

Ján Labuda ◽

Richard P. Bowater ◽

Miroslav Fojta ◽

Günter Gauglitz ◽

Zdeněk Glatz ◽

...

Keyword(s):

Recognition Element ◽

Biological Recognition

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Electrochemical nucleic acid-based biosensors: Concepts, terms, and methodology (IUPAC Technical Report)

Pure and Applied Chemistry ◽

10.1351/pac-rep-09-08-16 ◽

2010 ◽

Vol 82 (5) ◽

pp. 1161-1187 ◽

Cited By ~ 121

Author(s):

Jan Labuda ◽

Ana Maria Oliveira Brett ◽

Gennady Evtugyn ◽

Miroslav Fojta ◽

Marco Mascini ◽

...

Keyword(s):

Nucleic Acid ◽

Present Report ◽

Critical Evaluation ◽

Signal Transducer ◽

Wide Audience ◽

Recognition Element ◽

Technical Report ◽

Biological Recognition ◽

Electrochemical Signal ◽

And Performance

An electrochemical nucleic acid (NA)-based biosensor is a biosensor that integrates a nucleic acid as the biological recognition element and an electrode as the electrochemical signal transducer. The present report provides concepts, terms, and methodology related to biorecognition elements, detection principles, type of interactions to be addressed, and construction and performance of electrochemical NA biosensors, including their critical evaluation, which should be valuable for a wide audience, from academic, biomedical, environmental, and food-testing, drug-developing, etc. laboratories to sensor producers.

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