Numerous long single-stranded DNAs produced by dual amplification reactions for electrochemical detection of exosomal microRNAs

2020 ◽  
Vol 169 ◽  
pp. 112555
Author(s):  
Liang-Liang Wang ◽  
Wen-Qian Chen ◽  
Yu-Ru Wang ◽  
Lu-Peng Zeng ◽  
Ting-Ting Chen ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Dual Amplification

A novel electrochemical DNA detection method based on bio-barcode/gold label silver stain dual amplification

2020 ◽  
Vol 10 (2) ◽  
pp. 206-213
Author(s):  
Pengfei Jiang ◽  
Miduo Tan ◽  
Lan Zhao ◽  
Yulin Wang ◽  
Ting Pan ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Detection Method ◽  
Linear Sweep Voltammetry ◽  
Silica Nanoparticle ◽  
Silver Ions ◽  
Silver Deposition ◽  
Silver Stain ◽  
Modified Dna ◽  
Gold Label ◽  
Dual Amplification

A novel electrochemical gene detection method based on bio-barcode/gold label silver stain (GLSS) dual amplification is presented. The silica nanoparticle/gold nanoparticles (SiO2/AuNPs) barcode probes were constructed by combining SiO2 nanoparticle-labeled DNA with AuNPs, which hybridizes with target DNAs and the electrode surface-modified DNA probes. The silver ions were reduced on the surface of AuNPs to form a silver shell by the catalysis of AuNPs, and then the electrochemical detection of deposited silver was performed by linear sweep voltammetry (LSV). In this paper, the effects of electrode blocking agent, blocking time and silver deposition time on electrochemical detection were also investigated. The proposed electrochemical DNA biosensor bring forth a low detection limit of 0.23 fM and a widely linear range of 1 fM to 10 pM. This method can clearly distinguish the complementary and mismatched DNA, with good repeatability and stability.

Target-Triggered, Dual Amplification Strategy for Sensitive Electrochemical Detection of a Lymphoma-associated MicroRNA

2017 ◽  
Vol 236 ◽  
pp. 190-197 ◽  
Author(s):  
Amily Fang-Ju Jou ◽  
You-Jin Chen ◽  
Ying Li ◽  
Ying-Feng Chang ◽  
Jih-jong Lee ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Dual Amplification ◽  
Amplification Strategy
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