scholarly journals Label-free real-time monitoring of the BCR-triggered activation of primary human B cells modulated by the simultaneous engagement of inhibitory receptors

2021 ◽  
pp. 113469
Author(s):  
Kristof Kliment ◽  
Inna Szekacs ◽  
Beatrix Peter ◽  
Anna Erdei ◽  
Istvan Kurucz ◽  
...  
2013 ◽  
Vol 176 ◽  
pp. 1176-1182 ◽  
Author(s):  
Yuki Aonuma ◽  
Yasuhiko Kondo ◽  
Ayumi Hirano-Iwata ◽  
Atena Nishikawa ◽  
Yasuo Shinohara ◽  
...  

2018 ◽  
Vol 351 ◽  
pp. 80-89 ◽  
Author(s):  
Eniko Farkas ◽  
Andras Szekacs ◽  
Boglarka Kovacs ◽  
Marianna Olah ◽  
Robert Horvath ◽  
...  

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 478 ◽  
Author(s):  
Laura Pol ◽  
Chris Eckstein ◽  
Laura Acosta ◽  
Elisabet Xifré-Pérez ◽  
Josep Ferré-Borrull ◽  
...  

The chemical modification, or functionalization, of the surfaces of nanomaterials is a key step to achieve biosensors with the best sensitivity and selectivity. The surface modification of biosensors usually comprises several modification steps that have to be optimized. Real-time monitoring of all the reactions taking place during such modification steps can be a highly helpful tool for optimization. In this work, we propose nanoporous anodic alumina (NAA) functionalized with the streptavidin-biotin complex as a platform towards label-free biosensors. Using reflective interferometric spectroscopy (RIfS), the streptavidin-biotin complex formation, using biotinylated thrombin as a molecule model, was monitored in real-time. The study compared the performance of different NAA pore sizes in order to achieve the highest response. Furthermore, the optimal streptavidin concentration that enabled the efficient detection of the biotinylated thrombin attachment was estimated. Finally, the ability of the NAA-RIfS system to quantify the concentration of biotinylated thrombin was evaluated. This study provides an optimized characterization method to monitor the chemical reactions that take place during the biotinylated molecules attachment within the NAA pores.


2019 ◽  
Vol 244 ◽  
pp. 6-11 ◽  
Author(s):  
Aykut Arif Topçu ◽  
Erdoğan Özgür ◽  
Fatma Yılmaz ◽  
Nilay Bereli ◽  
Adil Denizli

Nanoscale ◽  
2014 ◽  
Vol 6 (7) ◽  
pp. 3561-3565 ◽  
Author(s):  
Sung Sik Lee ◽  
Luke P. Lee

We utilize nanoplasmonic optical imaging as the noninvasive and label-free method in order to monitorin vitroamyloid fibrogenesis in real-time, which is considered as the primary pathological mechanism of Alzheimer's disease.


2012 ◽  
Vol 32 (1) ◽  
pp. 250-258 ◽  
Author(s):  
Heinz-Georg Jahnke ◽  
Annett Braesigk ◽  
Till G.A. Mack ◽  
Sarah Pönick ◽  
Frank Striggow ◽  
...  

2010 ◽  
Vol 49 (32) ◽  
pp. 5476-5479 ◽  
Author(s):  
Brian G. Saar ◽  
Yining Zeng ◽  
Christian W. Freudiger ◽  
Yu-San Liu ◽  
Michael E. Himmel ◽  
...  

2011 ◽  
Vol 26 (5) ◽  
pp. 2728-2732 ◽  
Author(s):  
Sarah Milgram ◽  
Sandra Cortes ◽  
Marie-Bernadette Villiers ◽  
Patrice Marche ◽  
Arnaud Buhot ◽  
...  

2010 ◽  
Vol 122 (32) ◽  
pp. 5608-5611 ◽  
Author(s):  
Brian G. Saar ◽  
Yining Zeng ◽  
Christian W. Freudiger ◽  
Yu-San Liu ◽  
Michael E. Himmel ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document