Microarray analysis of MC3T3-E1 osteoblastic cell response to machined titanium surface and resorbable blast material titanium surface

Bone ◽  
2011 ◽  
Vol 48 ◽  
pp. S111 ◽  
Author(s):  
H. Lee ◽  
S.-H. Ko
Keyword(s):  
Microarray Analysis ◽  
Cell Response ◽  
Titanium Surface ◽  
Osteoblastic Cell

Human osteoblastic cell response to a Ca- and P-enriched titanium surface obtained by anodization

2009 ◽  
Vol 88A (4) ◽  
pp. 841-848 ◽  
Author(s):  
Rosemeire de Lordo Franco ◽  
Roberto Chiesa ◽  
Marcio Mateus Beloti ◽  
Paulo Tambasco de Oliveira ◽  
Adalberto Luiz Rosa
Keyword(s):  
Cell Response ◽  
Titanium Surface ◽  
Osteoblastic Cell ◽  
Human Osteoblastic Cell

Initial MC3T3-E1 cell response on magnesium ion implanted titanium surface

Bone ◽  
2010 ◽  
Vol 47 ◽  
pp. S125
Author(s):  
H. Lee ◽  
L.-R. Cho ◽  
C.-J. Park ◽  
H. Shin
Keyword(s):  
Cell Response ◽  
Titanium Surface ◽  
Magnesium Ion ◽  
Ion Implanted

scholarly journals Surface characteristics and osteoblastic cell response of alkali-and heat-treated titanium-8tantalum-3niobium alloy

2012 ◽  
Vol 42 (6) ◽  
pp. 248 ◽  
Author(s):  
Bo-Ah Lee ◽  
Choong-Hee Kang ◽  
Mong-Sook Vang ◽  
Young-Suk Jung ◽  
Xing Hui Piao ◽  
...  
Keyword(s):  
Cell Response ◽  
Surface Characteristics ◽  
Osteoblastic Cell ◽  
Heat Treated

Comparison of Gene-Expression of MC3T3-E1 Osteoblastic Cell Cultured on Machined Titanium Surface and Resorbable Blast Material Titanium Surface

2007 ◽  
Vol 361-363 ◽  
pp. 1095-1098
Author(s):  
S.H. Park ◽  
H.H. Kim ◽  
H.S. Lee ◽  
Y.S. Choi ◽  
A.R. Pae ◽  
...  
Keyword(s):  
Gene Expression ◽  
Titanium Surface ◽  
Cell Attachment ◽  
Single Gene ◽  
Rna Extraction ◽  
Genetic Effect ◽  
Osteoblastic Cell ◽  
Machined Surface ◽  
Osteoblastic Cell Line ◽  
Microarray Assay

Because of its high biocompatibility, hydroxyapatite(HA) has been considered as a good blasting material. DNA microarray is a new molecular technology that enables the analysis of gene expression in parallel on a very large number of genes, spanning a significant fraction of the human genome. It is a qualitive analysis (e.g. it can differentiate each single gene) and quantitative, since it has the sensitivity to detect a change of expression level in the investigated cells when compared to normal samples. The aim of this study is to define the cell attachment and the genetic effect of machined surface implant and RBM (resorbable blast media) surface implant on the osteoblastic cell (MC3T3-E1 osteoblastic cell line) by cDNA microarray slide containing 21575 genes. Cells were cultured on machined grade 4 titanium disks(Group 1, machined surface) and disks of RBM (Group 2) and the samples were moved to new dishes and media were added and the plated disks were cultured for 24 hours. Total RNA extraction was performed with Qiagen mini kit (Qiagen, Chatsworth, CA, USA) for microarray assay. Microarray assay after culturing the cells on the machined surface and RBM surface revealed that osteoinductive molecules appeared more prominent on the RBM surface, whereas the adhesion molecules on the biomaterial were higher on the machined surface than RBM surface.

Microarray-based expression analysis of human osteoblast-like cell response to anodized titanium surface

2004 ◽  
Vol 26 (5) ◽  
pp. 399-402 ◽  
Author(s):  
Yeonhee Kim ◽  
Jun-Hyeog Jang ◽  
Young Ku ◽  
Jae-Young Koak ◽  
Ik-Tae Chang ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Cell Response ◽  
Titanium Surface ◽  
Human Osteoblast ◽  
Anodized Titanium

scholarly journals Human foetal osteoblastic cell response to polymer-demixed nanotopographic interfaces

2005 ◽  
Vol 2 (2) ◽  
pp. 97-108 ◽  
Author(s):  
Jung Yul Lim ◽  
Joshua C Hansen ◽  
Christopher A Siedlecki ◽  
James Runt ◽  
Henry J Donahue
Keyword(s):  
Cell Response ◽  
Cell Function ◽  
Early Stage ◽  
Control Cell ◽  
Bone Cell ◽  
Cell Phenotype ◽  
Osteoblastic Cell ◽  
Bone Cell Differentiation ◽  
Biomaterial Interfaces ◽  
Cells Cultured

Nanoscale cell–substratum interactions are of significant interest in various biomedical applications. We investigated human foetal osteoblastic cell response to randomly distributed nanoisland topography with varying heights (11, 38 and 85 nm) produced by a polystyrene (PS)/polybromostyrene polymer-demixing technique. Cells displayed island-conforming lamellipodia spreading, and filopodia projections appeared to play a role in sensing the nanotopography. Cells cultured on 11 nm high islands displayed significantly enhanced cell spreading and larger cell dimensions than cells on larger nanoislands or flat PS control, on which cells often displayed a stellate shape. Development of signal transmitting structures such as focal adhesive vinculin protein and cytoskeletal actin stress fibres was more pronounced, as was their colocalization, in cells cultured on smaller nanoisland surfaces. Cell adhesion and proliferation were greater with decreasing island height. Alkaline phosphatase (AP) activity, an early stage marker of bone cell differentiation, also exhibited nanotopography dependence, i.e. higher AP activity on 11 nm islands compared with that on larger islands or flat PS. Therefore, randomly distributed island topography with varying nanoscale heights not only affect adhesion-related cell behaviour but also bone cell phenotype. Our results suggest that modulation of nanoscale topography may be exploited to control cell function at cell–biomaterial interfaces.

Evaluation of a Titanium Surface Treated with Hydroxyapatite Nanocrystals on Osteoblastic Cell Behavior: An In Vitro Study

2018 ◽  
Vol 33 (3) ◽  
pp. 597-602 ◽  
Author(s):  
Elizabeth Martinez ◽  
Guilherme Ishikawa ◽  
Alexandre de Lemos ◽  
Fábio Bezerra ◽  
Marcelo Sperandio ◽  
...  
Keyword(s):  
Titanium Surface ◽  
In Vitro Study ◽  
Vitro Study ◽  
Cell Behavior ◽  
Osteoblastic Cell ◽  
Hydroxyapatite Nanocrystals

Effect of RGD peptide coating of implant titanium surface on human mesenchymal stem cell response

2011 ◽  
Vol 49 (3) ◽  
pp. 245
Author(s):  
Min-Su Kim ◽  
Chang-Mo Jeong ◽  
Young-Chan Jeon ◽  
Jae-Jun Ryu ◽  
Jung-Bo Huh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Response ◽  
Titanium Surface ◽  
Human Mesenchymal Stem Cell ◽  
Rgd Peptide ◽  
Peptide Coating
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