Surface characteristics and osteoblastic cell response of alkali-and heat-treated titanium-8tantalum-3niobium alloy

In this study, nanotube morphology changes of Ti–xTa–Ag–Pt alloys with Ta content for biomaterials were researched using various experimental instruments. Ti–xTa–Ag–Pt alloys were manufactured in an Ar atmosphere using a vacuum arc-melting furnace with Ta contents of 10 and 50, and then heat-treated at 1100 °C for 1 hr. Nanotube formation of Ti–xTa–Ag–Pt (x = 10, 50 wt%) alloys were performed using a DC power of 30 V in 1.0 M H3PO4 + 0.8 wt% NaF electrolyte solution. Surface characteristics were investigated using an optical microscope, X-ray diffractometer, field-emission scanning electron microscope, energy-dispersive X-ray spectroscopy, and Image analyzer (Image J). Ti–10Ta–Ag–Pt alloy had a needle-like structures, and Ti–Ti–50Ta–Ag–Pt showed the mixed structure (equiaxed and needle-like structures). As the Ta content increased, the α-phase decreased and the β-phase increased. The highly ordered nanotubes were formed on the β-phase, whereas disordered nanotubes were formed on needle-like structure of α-phase in Ti–10Ta–Ag–Pt alloy. As the Ta content increases, large and small nanotube diameters became smaller in size. Anatase and rutile phases were formed on the alloy surface. Ta, Ag, and Pt elements were uniformly distributed over the entire surface and at the edge or inside of the nanotube.

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Response of osteoblastic cells to titanium submitted to three different surface treatments

Brazilian Oral Research ◽

10.1590/s1806-83242005000300009 ◽

2005 ◽

Vol 19 (3) ◽

pp. 203-208 ◽

Cited By ~ 15

Author(s):

Adriana Soares Santiago ◽

Euler Araújo Santos ◽

Márcia Soares Sader ◽

Marcelo Felippe Santiago ◽

Gloria de Almeida Soares

Keyword(s):

Cellular Proliferation ◽

Cell Attachment ◽

Pure Titanium ◽

Solution Treatment ◽

Surface Characteristics ◽

Osteoblastic Cell ◽

Commercially Pure Titanium ◽

Polar Components ◽

Chemical Attack ◽

Interface Surface

In the complex process of bone formation at the implant-tissue interface, surface properties are relevant factors modulating osteoblastic function. In this study, commercially pure titanium (cp Ti) samples were prepared with different surface characteristics using chemical attack with a sulfuric acid/hydrochloric acid based solution (treatment A); chemical attack plus anodic oxidation using phosphoric acid (treatment B); and chemical attack plus thermal oxidation followed by immersion in a sodium fluoride solution (treatment C). The samples were characterized by scanning electron microscopy (SEM), contact profilometry and contact angle. The biological performance of the prepared surfaces was evaluated using mice osteoblastic cell cultures for up to 21 days. Cells seeded on the different titanium samples showed similar behavior during cell attachment and spreading. However, cellular proliferation and differentiation were higher for samples submitted to treatments A and C (p < 0.05; n = 3), which were less rough and showed surface free energy with smaller polar components.

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Human foetal osteoblastic cell response to polymer-demixed nanotopographic interfaces

Journal of The Royal Society Interface ◽

10.1098/rsif.2004.0019 ◽

2005 ◽

Vol 2 (2) ◽

pp. 97-108 ◽

Cited By ~ 127

Author(s):

Jung Yul Lim ◽

Joshua C Hansen ◽

Christopher A Siedlecki ◽

James Runt ◽

Henry J Donahue

Keyword(s):

Cell Response ◽

Cell Function ◽

Early Stage ◽

Control Cell ◽

Bone Cell ◽

Cell Phenotype ◽

Osteoblastic Cell ◽

Bone Cell Differentiation ◽

Biomaterial Interfaces ◽

Cells Cultured

Nanoscale cell–substratum interactions are of significant interest in various biomedical applications. We investigated human foetal osteoblastic cell response to randomly distributed nanoisland topography with varying heights (11, 38 and 85 nm) produced by a polystyrene (PS)/polybromostyrene polymer-demixing technique. Cells displayed island-conforming lamellipodia spreading, and filopodia projections appeared to play a role in sensing the nanotopography. Cells cultured on 11 nm high islands displayed significantly enhanced cell spreading and larger cell dimensions than cells on larger nanoislands or flat PS control, on which cells often displayed a stellate shape. Development of signal transmitting structures such as focal adhesive vinculin protein and cytoskeletal actin stress fibres was more pronounced, as was their colocalization, in cells cultured on smaller nanoisland surfaces. Cell adhesion and proliferation were greater with decreasing island height. Alkaline phosphatase (AP) activity, an early stage marker of bone cell differentiation, also exhibited nanotopography dependence, i.e. higher AP activity on 11 nm islands compared with that on larger islands or flat PS. Therefore, randomly distributed island topography with varying nanoscale heights not only affect adhesion-related cell behaviour but also bone cell phenotype. Our results suggest that modulation of nanoscale topography may be exploited to control cell function at cell–biomaterial interfaces.

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Investigations on Surface Characteristics of Heat Treated Tool Steel after Wire Electro-Discharge Machining

Materials and Manufacturing Processes ◽

10.1080/10426914.2013.822976 ◽

2013 ◽

Vol 28 (10) ◽

pp. 1143-1146 ◽

Cited By ~ 12

Author(s):

M. M. Dhobe ◽

I. K. Chopde ◽

C. L. Gogte

Keyword(s):

Tool Steel ◽

Surface Characteristics ◽

Electro Discharge Machining ◽

Heat Treated

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