scholarly journals Changes in scaffold porosity during bone tissue engineering in perfusion bioreactors considerably affect cellular mechanical stimulation for mineralization

Bone Reports ◽  
2020 ◽  
Vol 12 ◽  
pp. 100265
Author(s):  
Feihu Zhao ◽  
Damien Lacroix ◽  
Keita Ito ◽  
Bert van Rietbergen ◽  
Sandra Hofmann
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Stimulation ◽  
Perfusion Bioreactors

Numerical Study of Granular Scaffold Efficiency to Convert Fluid Flow into Mechanical Stimulation in Bone Tissue Engineering

2015 ◽  
Vol 21 (9) ◽  
pp. 863-871 ◽  
Author(s):  
Magali Cruel ◽  
Morad Bensidhoum ◽  
Cécile Nouguier-Lehon ◽  
Olivier Dessombz ◽  
Pierre Becquart ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fluid Flow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Stimulation ◽  
Numerical Study

FOAM VERSUS FIBRE SCAFFOLDS FOR MECHANICAL STIMULATION OF CELLS IN BONE TISSUE ENGINEERING

2008 ◽  
Vol 41 ◽  
pp. S385
Author(s):  
David Clarke ◽  
Carme Coll ◽  
Anthony Ryan ◽  
Aileen Crawford ◽  
Gwendolen Reilly
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Stimulation ◽  
Stimulation Of

scholarly journals The role of perfusion bioreactors in bone tissue engineering

Biomatter ◽  
2012 ◽  
Vol 2 (4) ◽  
pp. 167-175 ◽  
Author(s):  
Diana Alves Gaspar ◽  
Viviane Gomide ◽  
Fernando Jorge Monteiro
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Perfusion Bioreactors

scholarly journals Use of Perfusion Bioreactors and Large Animal Models for Long Bone Tissue Engineering

2014 ◽  
Vol 20 (2) ◽  
pp. 126-146 ◽  
Author(s):  
Leandro S. Gardel ◽  
Luís A. Serra ◽  
Rui L. Reis ◽  
Manuela E. Gomes
Keyword(s):  
Tissue Engineering ◽  
Animal Models ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Long Bone ◽  
Large Animal ◽  
Large Animal Models ◽  
Perfusion Bioreactors

Computational modeling of media flow through perfusion-based bioreactors for bone tissue engineering

2020 ◽  
Vol 234 (12) ◽  
pp. 1397-1408
Author(s):  
Hanieh Nokhbatolfoghahaei ◽  
Mahboubeh Bohlouli ◽  
Kazem Adavi ◽  
Zahrasadat Paknejad ◽  
Maryam Rezai Rad ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Shear Stress ◽  
Fluid Flow ◽  
Computational Modeling ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Fluid Motion ◽  
Shear Loading ◽  
Perfusion Bioreactor ◽  
Perfusion Bioreactors

Bioreactor system has been used in bone tissue engineering in order to simulate dynamic nature of bone tissue environments. Perfusion bioreactors have been reported as the most efficient types of shear-loading bioreactor. Also, combination of forces, such as rotation plus perfusion, has been reported to enhance cell growth and osteogenic differentiation. Mathematical modeling using sophisticated infrastructure processes could be helpful and streamline the development of functional grafts by estimating and defining an effective range of bioreactor settings for better augmentation of tissue engineering. This study is aimed to conduct computational modeling for newly designed bioreactors in order to alleviate the time and material consuming for evaluating bioreactor parameters and effect of fluid flow hydrodynamics (various amounts of shear stress) on osteogenesis. Also, biological assessments were performed in order to validate similar parameters under implementing the perfusion or rotating and perfusion fluid motions in bioreactors’ prototype. Finite element method was used to investigate the effect of hydrodynamic of fluid flow inside the bioreactors. The equations used in the simulation to calculate the velocity values and consequently the shear stress values include Navier–Stokes and Brinkman equations. It has been shown that rotational fluid motion in rotating and perfusion bioreactor produces more velocity and shear stress compared with perfusion bioreactor. Moreover, implementing the perfusion together with rotational force in rotating and perfusion bioreactors has been shown to have more cell proliferation and higher activity of alkaline phosphatase enzyme as well as formation of extra cellular matrix sheet, as an indicator of bone-like tissue formation.

scholarly journals Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro

2018 ◽  
Vol 79 ◽  
pp. 232-237 ◽  
Author(s):  
Feihu Zhao ◽  
Bert van Rietbergen ◽  
Keita Ito ◽  
Sandra Hofmann
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Flow Rates ◽  
Perfusion Bioreactors

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

PLGA+HA/βTCP scaffold incorporating simvastatin: a promising biomaterial for bone tissue engineering

2020 ◽  
Author(s):  
Mariane Beatriz Sordi ◽  
Ariadne Cristiane Cabral da Cruz ◽  
Águedo Aragones ◽  
Mabel Mariela Rodríguez Cordeiro ◽  
Ricardo de Souza Magini
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Thermal Analyses ◽  
Chemical Properties ◽  
Biological Properties ◽  
Scanning Calorimetry ◽  
Evaporation Technique ◽  
Porosity Analysis ◽  
Biphasic Ceramic

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite / β-tricalcium phosphate (PLGA+HA/βTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% βTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m:m) of this medication. Scaffolds were synthesized in a cylindric-shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in PBS at 37 °C under constant stirring for 7, 14, 21, and 28 days. Non-degraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro, meso, and micropores in the polymer structure with HA/βTCP particles homogeneously dispersed. Chemical and thermal analyses presented very similar results for both PLGA+HA/βTCP and PLGA+HA/βTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/βTCP and PLGA+HA/βTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/βTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.

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