Nanonet Inter-Fiber Spacing Controls Plasticity in Cell Migration

We are developing an in vitro interstitial extracellular matrix (ECM) system for study of inflammatory cell migration. Falcon brand Cyclopore membrane inserts of various pore sizes are used as a support substrate for production of ECM by R22 rat aortic smooth muscle cells. Under specific culture conditions these cells produce a highly insoluble matrix consisting of typical interstitial ECM components, i.e.: types I and III collagen, elastin, proteoglycans and fibronectin.

Download Full-text

The role of DUBs in the post-translational control of cell migration

Essays in Biochemistry ◽

10.1042/ebc20190022 ◽

2019 ◽

Vol 63 (5) ◽

pp. 579-594 ◽

Cited By ~ 2

Author(s):

Guillem Lambies ◽

Antonio García de Herreros ◽

Víctor M. Díaz

Keyword(s):

Cell Migration ◽

Translational Control ◽

Epithelial To Mesenchymal Transition ◽

Extracellular Matrix Remodeling ◽

Matrix Remodeling ◽

Mesenchymal Transition ◽

Tgfβ Receptors ◽

Fundamental Process ◽

Multistep Process

Abstract Cell migration is a multifactorial/multistep process that requires the concerted action of growth and transcriptional factors, motor proteins, extracellular matrix remodeling and proteases. In this review, we focus on the role of transcription factors modulating Epithelial-to-Mesenchymal Transition (EMT-TFs), a fundamental process supporting both physiological and pathological cell migration. These EMT-TFs (Snail1/2, Twist1/2 and Zeb1/2) are labile proteins which should be stabilized to initiate EMT and provide full migratory and invasive properties. We present here a family of enzymes, the deubiquitinases (DUBs) which have a crucial role in counteracting polyubiquitination and proteasomal degradation of EMT-TFs after their induction by TGFβ, inflammatory cytokines and hypoxia. We also describe the DUBs promoting the stabilization of Smads, TGFβ receptors and other key proteins involved in transduction pathways controlling EMT.

Download Full-text

The Level of C/EBP Protein Is Critical for Cell Migration during Drosophila Oogenesis and Is Tightly Controlled by Regulated Degradation

Molecular Cell ◽

10.1016/s1097-2765(00)00004-6 ◽

2000 ◽

Vol 6 (1) ◽

pp. 23-30 ◽

Cited By ~ 81

Author(s):

P RORTH

Keyword(s):

Cell Migration ◽

Drosophila Oogenesis

Download Full-text

Author response for "IL‐17 regulates dendritic cell migration to the peribronchial lymph nodes and allergen presentation in experimental allergic asthma"

10.1002/eji.201948409/v2/response1 ◽

2020 ◽

Author(s):

Adan Chari Jirmo ◽

Mandy Busse ◽

Christine Happle ◽

Jelena Skuljec ◽

Kathleen Dalüge ◽

...

Keyword(s):

Cell Migration ◽

Dendritic Cell ◽

Lymph Nodes ◽

Allergic Asthma ◽

Author Response ◽

Dendritic Cell Migration ◽

Experimental Allergic Asthma ◽

Experimental Allergic

Download Full-text

Decision letter for "IL‐17 regulates dendritic cell migration to the peribronchial lymph nodes and allergen presentation in experimental allergic asthma"

10.1002/eji.201948409/v1/decision1 ◽

2019 ◽

Keyword(s):

Cell Migration ◽

Dendritic Cell ◽

Lymph Nodes ◽

Allergic Asthma ◽

Dendritic Cell Migration ◽

Experimental Allergic Asthma ◽

Experimental Allergic

Download Full-text

Cell migration: Two steps at a time

Nature China ◽

10.1038/nchina.2009.127 ◽

2009 ◽

Author(s):

Felix Cheung

Keyword(s):

Cell Migration

Download Full-text

Guidance mechanisms of the cell migration in the cerebral cortex

Neuroscience Research ◽

10.1016/s0168-0102(00)80956-6 ◽

2000 ◽

Vol 38 ◽

pp. S18 ◽

Cited By ~ 1

Author(s):

N Tamamaki

Keyword(s):

Cerebral Cortex ◽

Cell Migration

Download Full-text

Monitoring stem cell migration in the nervous system by in vivo magnetic resonance imaging

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/sj.jcbfm.9591524.0693 ◽

2005 ◽

Vol 25 (1_suppl) ◽

pp. S692-S692

Author(s):

Mathias Hoehn ◽

Uwe Himmelreich ◽

Ralph Weber ◽

Pedro Ramos-Cabrer ◽

Susanne Wegener ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Nervous System ◽

Stem Cell ◽

Cell Migration ◽

Magnetic Resonance ◽

Resonance Imaging ◽

Stem Cell Migration

Download Full-text

The Migration of Human Smooth Muscle Cells In Vitro Is Mediated by Plasminogen Activation and Can Be Inhibited by α2-Macroglobulin Receptor Associated Protein

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657646 ◽

1997 ◽

Vol 78 (02) ◽

pp. 880-886 ◽

Cited By ~ 46

Author(s):

Monique J Wijnberg ◽

Paul H A Quax ◽

Nancy M E Nieuwenbroek ◽

Jan H Verheijen

Keyword(s):

Smooth Muscle ◽

Cell Migration ◽

Smooth Muscle Cell ◽

Muscle Cell ◽

Ldl Receptor ◽

Migration And Invasion ◽

Invasion Assay ◽

Plasminogen Activation ◽

Smooth Muscle Cell Migration

SummaryThe plasminogen activation system is thought to be important in cell migration processes. A role for this system during smooth muscle cell migration after vascular injury has been suggested from several animal studies. However, not much is known about its involvement in human vascular remodelling. We studied the involvement of the plasminogen activation system in human smooth muscle cell migration in more detail using an in vitro wound assay and a matrix invasion assay. Inhibition of plasmin activity or inhibition of urokinase-type plasminogen activator (u-PA) activity resulted in approximately 40% reduction of migration after 24 h in the wound assay and an even stronger reduction (70-80%) in the matrix invasion assay. Migration of smooth muscle cells in the presence of inhibitory antibodies against tissue-type plasminogen activator (t-PA) was not significantly reduced after 24 h, but after 48 h a 30% reduction of migration was observed, whereas in the matrix invasion assay a 50% reduction in invasion was observed already after 24 h. Prevention of the interaction of u-PA with cell surface receptors by addition of soluble u-PA receptor or α2-macroglobulin receptor associated protein (RAP) to the culture medium, resulted in a similar inhibition of migration and invasion. From these results it can be concluded that both u-PA and t-PA mediated plasminogen activation can contribute to in vitro human smooth muscle cell migration and invasion. Furthermore, the interaction between u-PA and its cell surface receptor appears also to be involved in this migration and invasion process. The inhibitory effects on migration and invasion by the addition of RAP suggests an involvement of a RAP sensitive receptor of the LDL receptor family, possibly the LDL-receptor related protein (LRP) and/or the VLDL receptor.

Download Full-text