scholarly journals Folding Kinetics of Multiple G-Quadruplex Telomeric DNA Structures

2020 ◽  
Vol 118 (3) ◽  
pp. 335a
Author(s):  
Emil L. Kristoffersen ◽  
Andrea Coletta ◽  
Line Lund ◽  
Birgit Schiøtt ◽  
Victoria Birkedal
Keyword(s):  
Folding Kinetics ◽  
Telomeric Dna ◽  
Dna Structures ◽  
G Quadruplex ◽  
Kinetics Of

scholarly journals Folding Kinetics of G-Quadruplexes: Duplex Stem Loops Drive and Accelerate G-Quadruplex Folding

2020 ◽  
Vol 124 (25) ◽  
pp. 5122-5130 ◽  
Author(s):  
Thi Quynh Ngoc Nguyen ◽  
Kah Wai Lim ◽  
Anh Tuân Phan
Keyword(s):  
Folding Kinetics ◽  
G Quadruplex ◽  
Kinetics Of

Ultrafast Microfluidic Mixer for Tracking the Early Folding Kinetics of Human Telomere G-Quadruplex

2014 ◽  
Vol 86 (9) ◽  
pp. 4333-4339 ◽  
Author(s):  
Ying Li ◽  
Chao Liu ◽  
Xiaojun Feng ◽  
Youzhi Xu ◽  
Bi-Feng Liu
Keyword(s):  
Folding Kinetics ◽  
Microfluidic Mixer ◽  
G Quadruplex ◽  
Human Telomere ◽  
Kinetics Of

scholarly journals Sequence and Solvent Effects on Telomeric DNA Bimolecular G-Quadruplex Folding Kinetics

2013 ◽  
Vol 117 (41) ◽  
pp. 12391-12401 ◽  
Author(s):  
Adrien Marchand ◽  
Rubén Ferreira ◽  
Hisae Tateishi-Karimata ◽  
Daisuke Miyoshi ◽  
Naoki Sugimoto ◽  
...  
Keyword(s):  
Solvent Effects ◽  
Folding Kinetics ◽  
Telomeric Dna ◽  
G Quadruplex

scholarly journals Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance in Saccharomyces cerevisiae

2020 ◽  
Vol 295 (27) ◽  
pp. 8958-8971 ◽  
Author(s):  
Katarina Jurikova ◽  
Martin Gajarsky ◽  
Mona Hajikazemi ◽  
Jozef Nosek ◽  
Katarina Prochazkova ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Secondary Structures ◽  
Telomere Maintenance ◽  
Folding Kinetics ◽  
Mobility Shift ◽  
Dna Structures ◽  
Electrophoretic Mobility Shift Assays ◽  
Kinetics Of

The ends of eukaryotic chromosomes typically contain a 3′ ssDNA G-rich protrusion (G-overhang). This overhang must be protected against detrimental activities of nucleases and of the DNA damage response machinery and participates in the regulation of telomerase, a ribonucleoprotein complex that maintains telomere integrity. These functions are mediated by DNA-binding proteins, such as Cdc13 in Saccharomyces cerevisiae, and the propensity of G-rich sequences to form various non-B DNA structures. Using CD and NMR spectroscopies, we show here that G-overhangs of S. cerevisiae form distinct Hoogsteen pairing–based secondary structures, depending on their length. Whereas short telomeric oligonucleotides form a G-hairpin, their longer counterparts form parallel and/or antiparallel G-quadruplexes (G4s). Regardless of their topologies, non-B DNA structures exhibited impaired binding to Cdc13 in vitro as demonstrated by electrophoretic mobility shift assays. Importantly, whereas G4 structures formed relatively quickly, G-hairpins folded extremely slowly, indicating that short G-overhangs, which are typical for most of the cell cycle, are present predominantly as single-stranded oligonucleotides and are suitable substrates for Cdc13. Using ChIP, we show that the occurrence of G4 structures peaks at the late S phase, thus correlating with the accumulation of long G-overhangs. We present a model of how time- and length-dependent formation of non-B DNA structures at chromosomal termini participates in telomere maintenance.

Is There Any Interaction Between Telomeric DNA Structures, G-Quadruplex and I-Motif, with Saffron Active Metabolites?

2012 ◽  
Vol 31 (11) ◽  
pp. 801-812 ◽  
Author(s):  
Reyhane Hoshyar ◽  
S. Zahra Bathaie ◽  
Anahita Kyani ◽  
Mir Fazlollah Mousavi
Keyword(s):  
Telomeric Dna ◽  
Active Metabolites ◽  
Dna Structures ◽  
G Quadruplex

scholarly journals Folding Kinetics of Single Human Telomeric G-Quadruplex Affected by Cisplatin

ACS Omega ◽  
2016 ◽  
Vol 1 (2) ◽  
pp. 244-250 ◽  
Author(s):  
Hai-Peng Ju ◽  
Yi-Zhou Wang ◽  
Jing You ◽  
Xi-Miao Hou ◽  
Xu-Guang Xi ◽  
...  
Keyword(s):  
Folding Kinetics ◽  
G Quadruplex ◽  
Kinetics Of

scholarly journals Effects of monovalent cations on folding kinetics of G-quadruplexes

2017 ◽  
Vol 37 (4) ◽  
Author(s):  
Jing You ◽  
Hui Li ◽  
Xi-Ming Lu ◽  
Wei Li ◽  
Peng-Ye Wang ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Genome Stability ◽  
Structural Transition ◽  
Monovalent Cation ◽  
Monovalent Cations ◽  
Folding Kinetics ◽  
Folding Rates ◽  
Type K ◽  
G Quadruplex ◽  
Kinetics Of

G-quadruplexes are special structures existing at the ends of human telomeres, the folding kinetics of which are essential for their functions, such as in the maintenance of genome stability and the protection of chromosome ends. In the present study, we investigated the folding kinetics of G-quadruplex in different monovalent cation environments and determined the detailed kinetic parameters for Na+- and K+-induced G-quadruplex folding, and for its structural transition from the basket-type Na+ form to the hybrid-type K+ form. More interestingly, although Li+ was often used in previous studies of G-quadruplex folding as a control ion supposed to have no effect, we have found that Li+ can actually influence the folding kinetics of both Na+- and K+-induced G-quadruplexes significantly and in different ways, by changing the folding fraction of Na+-induced G-quadruplexes and greatly increasing the folding rates of K+-induced G-quadruplexes. The present study may shed new light on the roles of monovalent cations in G-quadruplex folding and should be useful for further studies of the underlying folding mechanism.

Sign in / Sign up

Export Citation Format

Share Document