Microarray assay of circular RNAs reveals cicRNA.7079 as a new anti-apoptotic molecule in spinal cord injury in mice

Spinal Cord Injury (SCI) can elicit a progressive loss of nerve cells promoting disability, morbidity, and even mortality. Despite different triggering mechanisms, a cascade of molecular events involving complex gene alterations and activation of the neuroimmune system influence either cell damage or repair. Effective therapies to avoid secondary mechanisms underlying SCI are still lacking. The recent progression in circular RNAs (circRNAs) research has drawn increasing attention and opened a new insight on SCI pathology. circRNAs differ from traditional linear RNAs and have emerged as the active elements to regulate gene expression as well as to facilitate the immune response involved in pathophysiology-related conditions. In this review, we focus on the impact and possible close relationship of circRNAs with pathophysiological mechanisms following SCI, where circRNAs could be the key transcriptional regulatory molecules to define neuronal death or survival. Advances in circRNAs research provide new insight on potential biomarkers and effective therapeutic targets for SCI patients.

Download Full-text

Screening Circular RNA Related to Vascular Endothelial Proliferation, Migration and Angiogenesis in Spinal Cord Injury by RNA Sequencing

10.21203/rs.3.rs-127498/v1 ◽

2020 ◽

Author(s):

Xin Ye ◽

Yilei Chen ◽

Jiasheng Wang ◽

Jian Chen ◽

Ying Yao ◽

...

Keyword(s):

Gene Expression ◽

Spinal Cord ◽

Spinal Cord Injury ◽

Early Stage ◽

Expression Profiles ◽

Expression Patterns ◽

Circular Rnas ◽

Vascular Endothelial ◽

Endothelial Proliferation ◽

Cord Injury

Abstract Background: Traumatic spinal cord injury (SCI) causes high rates of worldwide morbidity because of the complex secondary injury. Circular RNAs (circRNAs) are a novel class of endogenous non-coding RNAs, which have recently been recognized as important regulators of gene expression and pathological processes. In this study, we have attempted to elucidate the expression profiles of circRNAs in a mouse model of SCI and comprehensively understand vascular endothelial proliferation, migration and angiogenesis in the early stage of secondary injury.Methods: Deep RNA sequencing (RNA-seq) and bioinformatic analysis including GO enrichment analysis, KEGG pathway analysis and circRNA-miRNA-mRNA network construction were performed to investigate the expression patterns of circRNAs in mouse spinal cord after SCI (n= 3 per group) for three days and explore the differentially expressed circRNAs related to vascular endothelial proliferation, migration and angiogenesis. Results: Total of 1288 circRNAs were altered (>2-fold change, p<0.05) in the spinal cord after SCI, including 991 were upregulated and 297 were downregulated. Meanwhile we constructed a circRNA-mRNA network to predict their functions for circRNAs can act as “miRNA sponges”,. We next analyzed the altered circRNAs related to vascular endothelial proliferation, migration and angiogenesis by GO and KEGG analyses. 121 circRNAs were found to correlating to vascular endothelial proliferation，migration and angiogenesis in spinal cord after SCI. Conclusions: Our results reveal that circRNAs locally regulate their related protein-gene expression and play key roles in the vascular endothelial proliferation, migration and angiogenesis of SCI.

Download Full-text

The emerging role of circular RNAs in spinal cord injury

Journal of Orthopaedic Translation ◽

10.1016/j.jot.2021.06.001 ◽

2021 ◽

Vol 30 ◽

pp. 1-5

Author(s):

Peng Peng ◽

Hao Yu ◽

Yongjin Li ◽

Jingyuan Huang ◽

Shengyu Yao ◽

...

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Circular Rnas ◽

Cord Injury

Download Full-text

The potential roles of circular RNAs as modulators in traumatic spinal cord injury

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2021.111826 ◽

2021 ◽

Vol 141 ◽

pp. 111826

Author(s):

Bin Lv ◽

Kaiyang Wang ◽

Tao Xu ◽

Jishan Yuan ◽

Dan Wang ◽

...

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Circular Rnas ◽

Traumatic Spinal Cord Injury ◽

Cord Injury

Download Full-text

Identification of Circular RNAs Related to Vascular Endothelial Proliferation, Migration, and Angiogenesis After Spinal Cord Injury Using Microarray Analysis in Female Mice

Frontiers in Neurology ◽

10.3389/fneur.2021.666750 ◽

2021 ◽

Vol 12 ◽

Author(s):

Xin Ye ◽

Yilei Chen ◽

Jiasheng Wang ◽

Jian Chen ◽

Ying Yao ◽

...

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Microarray Analysis ◽

Expression Profiles ◽

Circular Rnas ◽

Vascular Endothelial ◽

Female Mice ◽

Endothelial Proliferation ◽

Cord Injury

Background: Traumatic spinal cord injury (SCI) can result in severe disability and causes a considerable socio-economic burden worldwide. Circular RNAs (circRNAs) are important regulators of gene expression and pathological processes, and may represent therapeutic targets for SCI. To further evaluate the role of circRNAs in SCI, we elucidated circRNA expression profiles related to vascular endothelial proliferation, migration, and angiogenesis during the early stages of secondary injury in a mouse model of SCI.Methods: Microarray analysis was performed to investigate the circRNA expression patterns in the spinal cord 3 days after SCI in female mice. Bioinformatic analyses, including GO enrichment analysis, KEGG pathway analysis, and circRNA-miRNA-mRNA network construction, were conducted to explore the role of circRNA dysregulation in vascular endothelial proliferation, migration, and angiogenesis following SCI.Results: The expression of 1,288 circRNAs was altered (>2-fold change, p < 0.05) in the spinal cord after SCI, consisting of 991 upregulated and 297 downregulated circRNAs. We constructed a circRNA-mRNA network to predict whether these circRNAs could act as “miRNA sponges.” We next assessed the association of altered circRNAs with vascular endothelial proliferation, migration, and angiogenesis using GO and KEGG analyses. Using this analysis, we found that a total of 121 circRNAs were correlated with vascular endothelial proliferation, migration, and angiogenesis in the spinal cord after SCI.Conclusions: Our study provides circRNA expression profiles during the early stages of SCI. circRNA.7079, circRNA.7078, and circRNA.6777 were found to play key roles in the vascular endothelial proliferation, migration, and angiogenesis, and may represent therapeutic targets for SCI.

Download Full-text