An advanced intelligent ELISA test for bovine tuberculosis diagnosis

2018 ◽  
Vol 46 ◽  
pp. 59-66 ◽  
Author(s):  
Hanene Sahli ◽  
Aymen Mouelhi ◽  
Mohamed Fethi Diouani ◽  
Lotfi Tlig ◽  
Amira Refai ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Elisa Test ◽  
Tuberculosis Diagnosis

scholarly journals Influence of the incubation conditions on culture media to optimize primary isolation of Mycobacterium bovis

2016 ◽  
Vol 37 (5Supl2) ◽  
pp. 3693
Author(s):  
Cássia Yumi Ikuta ◽  
Flávia Morato ◽  
Gisele Oliveira de Souza ◽  
Marcos Bryan Heinemann ◽  
Marcos Amaku ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Culture Media ◽  
Growth Time ◽  
Solid Culture ◽  
Sodium Pyruvate ◽  
Primary Isolation ◽  
Granulomatous Lesions ◽  
Tuberculosis Diagnosis ◽  
Carbon Dioxide Co2

The isolation of Mycobacterium bovis is critical to a surveillance system for bovine tuberculosis based on detection of lesions in abattoirs. Thus, four solid culture media and three incubation conditions were investigated to elucidate which combination overcomes the others by assessing growth, time to the first appearance of colonies and their number. Ninety-seven samples of granulomatous lesions were submitted to the decontamination procedure by 1-hexadecylpyridinium chloride at 0.75% w/v, and inoculated on two egg-based media, Stonebrink’s (ST) and Löwenstein-Jensen’s with sodium pyruvate (LJp), and two agar-based media, tuberculosis blood agar (B83) and Middlebrook 7H11 medium (7H11). Each medium was incubated at 37°C for 90 days in three incubation conditions: in air, in air containing 10% carbon dioxide (CO2), and in air in slopes closed with burned hydrophobic cotton and subsequently plugged with a cork to create a microaerophilic atmosphere. The colonies appeared faster and in higher number when incubated in air containing 10% CO2 (p < 0.01), independent of media. B83 showed a faster growth and detected more isolates at 30 days of incubation, when compared to ST (0.0178), LJp (p < 0.0001) and 7H11 (p < 0.0001), though there was no difference between B83, ST and LJp at 60 and 90 days of incubation. 7H11 presented the lowest number of isolates (p < 0.0001) and a longer period for the appearance of the first colony (p < 0.001). According to our findings, the concomitant use of ST and B83 media incubated in air containing 10% CO2 increases the isolation of M. bovis in a shorter period of time, which improves bovine tuberculosis diagnosis.

scholarly journals A comparison between indirect ELISA and tuberculin skin test in the diagnosis of bovine tuberculosis in Kenya

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Daniel Muasya ◽  
George Karuoya Gitau ◽  
Thaiyah Gitau Andrew ◽  
Daniel Waweru Gakuya ◽  
John Vanleeuwen ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Kappa Statistics ◽  
Fisher Exact Test ◽  
Limited Information ◽  
Serum Samples ◽  
Apparent Prevalence ◽  
Kappa Agreement

Bovine tuberculosis (BTB) is an important zoonotic disease which has remained persistent in many areas of the World. Control and eradication has proved problematic due to the challenges in effective screening and diagnosis. Limited information on the status of BTB in Kenya presents a need to investigate its occurrence in cattle population. The study was carried out using an indirect antibody Enzyme Linked Immunosorbent Assay (ELISA-IDEXX-USA) to assess the agreement with Comparative Intradermal Tuberculin Test (CITT) test results between August and December 2013 in Laikipia County. The study also determined the apparent prevalence of cattle BTB antibodies in Laikipia County. A total of 276 bovine serum samples were tested using MPB70 and MPB83 recombinant proteins as capture antigens in the ELISA kit. Data were recorded in Microsoft Excel and exported to SPSS 16.0 for analysis. Apparent prevalence was calculated as a proportion at 95% CI, Kappa statistics computed, and test of significance assessed by Chi-square and Fisher exact test.  The results showed an individual animal apparent prevalence of 3.9% (11/276) and a herd prevalence of 58.3% (7/12). There was no significant association between BTB infection and both animal level factors, breed and sex. Kappa agreement test between ELISA test and TST showed a good agreement at K= 0.65. This study reported the prevalence of BTB in cattle for the first time in Laikipia County Kenya using the IDEXX ELISA. The ELISA test was comparable to the tuberculin test which is used as the gold standard for screening TB on live animals. The two tests can be used alongside for series or parallel interpretation to achieve desirable diagnostic sensitivity and specificity in disease control programs.

scholarly journals More on search for causes of sensitization to tuberculin PPD for mammals in cattle

2021 ◽  
pp. 271-276
Author(s):  
M. O. Baratov ◽  
P. S. Huseynova
Keyword(s):  
Differential Diagnosis ◽  
Bovine Tuberculosis ◽  
Nontuberculous Mycobacterium ◽  
Clinical Tests ◽  
Postmortem Diagnosis ◽  
Significance Level ◽  
Acid Tolerant ◽  
Tuberculosis Diagnosis ◽  
Trematode Infections ◽  
Insight Into

Despite the large number of papers dealing with the description of proposed methods for bovine tuberculosis diagnosis and mechanisms of non-specific reaction development in diseased and healthy animals, various aspects require further study. Many specialists are still of the view, formulated when studying causes of pseudoallergic reactions, that the agents of actinomycosis, trematode infections etc. can cause sensitization of the animal body to tuberculin PPD for mammals. The possibility of sensitization of cattle body to Аctinomyces bovis tuberculin was studied in 240 animals identified as actinomycosis diseased among 3,473 tested animals. Only 11 (4.6%) of the total number of diseased animals were reactors to tuberculin PPD for mammals. During bacteriological tests of material from animals euthanized for diagnostic purposes (tuberculin reactors and nonreactors with a confirmed postmortem diagnosis of actinomycosis), acid-tolerant nontuberculous mycobacterium (NTM) cultures were isolated. The results of the experiment conducted in 628 cows of a dairy holding confirmed that Actinomyces bovis lacks tuberculin-associated allergenicity: actinomycosis was detected only in one of 96 (15.2%) tuberculin reactors. The conducted clinical tests with high significance level (Р < 0.005) showed that there is no association between allergic reaction to tuberculin PPD for mammals and actinomycosis infection. The obtained results are indicative of imperfections in differential diagnosis, and further studies in this field should significantly contribute to gaining a better insight into non-specific sensitization of cattle body to tuberculin.

A comparison between indirect ELISA and tuberculin skin test in the diagnosis of bovine tuberculosis in Kenya

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Daniel Muasya ◽  
George Karuoya Gitau ◽  
Thaiyah Gitau Andrew ◽  
Daniel Waweru Gakuya ◽  
John Vanleeuwen ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Kappa Statistics ◽  
Fisher Exact Test ◽  
Limited Information ◽  
Serum Samples ◽  
Apparent Prevalence ◽  
Kappa Agreement

Bovine tuberculosis (BTB) is an important zoonotic disease which has remained persistent in many areas of the World. Control and eradication has proved problematic due to the challenges in effective screening and diagnosis. Limited information on the status of BTB in Kenya presents a need to investigate its occurrence in cattle population. The study was carried out using an indirect antibody Enzyme Linked Immunosorbent Assay (ELISA-IDEXX-USA) to assess the agreement with Comparative Intradermal Tuberculin Test (CITT) test results between August and December 2013 in Laikipia County. The study also determined the apparent prevalence of cattle BTB antibodies in Laikipia County. A total of 276 bovine serum samples were tested using MPB70 and MPB83 recombinant proteins as capture antigens in the ELISA kit. Data were recorded in Microsoft Excel and exported to SPSS 16.0 for analysis. Apparent prevalence was calculated as a proportion at 95% CI, Kappa statistics computed, and test of significance assessed by Chi-square and Fisher exact test.  The results showed an individual animal apparent prevalence of 3.9% (11/276) and a herd prevalence of 58.3% (7/12). There was no significant association between BTB infection and both animal level factors, breed and sex. Kappa agreement test between ELISA test and TST showed a good agreement at K= 0.65. This study reported the prevalence of BTB in cattle for the first time in Laikipia County Kenya using the IDEXX ELISA. The ELISA test was comparable to the tuberculin test which is used as the gold standard for screening TB on live animals. The two tests can be used alongside for series or parallel interpretation to achieve desirable diagnostic sensitivity and specificity in disease control programs.

scholarly journals Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis

Tuberculosis ◽  
2015 ◽  
Vol 95 (6) ◽  
pp. 795-801 ◽  
Author(s):  
María E. Eirin ◽  
Analia Macias ◽  
Gabriel Magnano ◽  
Claudia Morsella ◽  
Laura Mendez ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Interferon Gamma ◽  
Bovine Tuberculosis ◽  
Interferon Gamma Release Assay ◽  
Release Assay ◽  
Tuberculosis Diagnosis

scholarly journals Comparison between tests for tuberculosis diagnosis in slaughtered bovines

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
David Attuy Vey da Silva ◽  
Márcio Junio Lima Siconelli ◽  
Karina Paes Bürger ◽  
Lara Borges Keid
Keyword(s):  
Bovine Tuberculosis ◽  
Dna Detection ◽  
Bacterial Isolation ◽  
Solid Culture ◽  
Chain Reaction ◽  
Bacterial Dna ◽  
Tuberculosis Diagnosis ◽  
Polymerase Chain ◽  
Low Sensitivity ◽  
Different Sources

ABSTRACT: Our goal for this article is to compare several different diagnosis tests for bovine tuberculosis identification. We have performed bacterial isolation, histopathological characterization, acid-fast bacilli (AFB) identification and M. bovis DNA detection. Lesions suggestive of Tuberculosis were sampled from bovine lymph nodes during slaughtering of bovines at an abattoir that operates under federal inspection. The bacterial isolation was performed in solid culture mediums, the histopathological characterization was made by Hematoxylin-eosinstaining, and AFB identification by Ziehl-Neelsen staining. Bacterial DNA detection was performed by Polymerase Chain Reaction (PCR) using DNA from two different sources, directly collected from the tuberculosis-like lesions (PCR followed by nested PCR) and from isolated bacteria. We have concluded that the multi-step approach, including histopathological characterization, bacterial isolation and AFB identification, is strongly recommended to diagnose tuberculosis in bovines. Furthermore, PCR assays using specimens of lesions suggestive of tuberculosis are a faster and more promising way to diagnose the disease. However, it should not be used alone due to the low sensitivity shown in this study.

scholarly journals Comparative Identification of Mycobacterium bovis By Using A Serological ELISA (IDEXX) and Tuberculin Test in Cattle in Wasit Province /Iraq

2018 ◽  
Vol 1 (24) ◽  
pp. 459-472
Author(s):  
Hala SR. AL-Taee
Keyword(s):  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Endemic Area ◽  
Age Groups ◽  
Tuberculin Test ◽  
Diagnostic Techniques ◽  
Elisa Test ◽  
Scale Dependence ◽  
Positive Results ◽  
Two Age Groups

In Iraq, due to semi-full-scale dependence on tuberculin test, and presence, only, limited serological and molecular studies, the practical Mycobacterium bovis infection rate still dispersed especially in Wasit province that consider as an endemic area for bovine tuberculosis. In this study, two diagnostic techniques were used, IDEXX ELISA and tuberculin test; 186 cattle (155 females and 31 males), selected randomly and divided into two age groups, the first with less than 5 years and the second with more than 5 years. All cattle submitted to both tests and the results showed that (9.13%) were positives for tuberculin test and (17.2%) for IDEXX ELISA. Also, in age groups, the result of <5 years group by tuberculin test was 5.04%, and 11.76% by IDEXX ELISA test; while in >5 years group, was 16.41% and 26.86%, by tuberculin test and IDEXX ELISA test, respectively. Significant, the statistical different, P£ 0.05, was reported through the positive results of both tests as well as the two age groups, while in sex groups, the variance had not shown through both sexes.

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