scholarly journals More on search for causes of sensitization to tuberculin PPD for mammals in cattle

2021 ◽  
pp. 271-276
Author(s):  
M. O. Baratov ◽  
P. S. Huseynova
Keyword(s):  
Differential Diagnosis ◽  
Bovine Tuberculosis ◽  
Nontuberculous Mycobacterium ◽  
Clinical Tests ◽  
Postmortem Diagnosis ◽  
Significance Level ◽  
Acid Tolerant ◽  
Tuberculosis Diagnosis ◽  
Trematode Infections ◽  
Insight Into

Despite the large number of papers dealing with the description of proposed methods for bovine tuberculosis diagnosis and mechanisms of non-specific reaction development in diseased and healthy animals, various aspects require further study. Many specialists are still of the view, formulated when studying causes of pseudoallergic reactions, that the agents of actinomycosis, trematode infections etc. can cause sensitization of the animal body to tuberculin PPD for mammals. The possibility of sensitization of cattle body to Аctinomyces bovis tuberculin was studied in 240 animals identified as actinomycosis diseased among 3,473 tested animals. Only 11 (4.6%) of the total number of diseased animals were reactors to tuberculin PPD for mammals. During bacteriological tests of material from animals euthanized for diagnostic purposes (tuberculin reactors and nonreactors with a confirmed postmortem diagnosis of actinomycosis), acid-tolerant nontuberculous mycobacterium (NTM) cultures were isolated. The results of the experiment conducted in 628 cows of a dairy holding confirmed that Actinomyces bovis lacks tuberculin-associated allergenicity: actinomycosis was detected only in one of 96 (15.2%) tuberculin reactors. The conducted clinical tests with high significance level (Р < 0.005) showed that there is no association between allergic reaction to tuberculin PPD for mammals and actinomycosis infection. The obtained results are indicative of imperfections in differential diagnosis, and further studies in this field should significantly contribute to gaining a better insight into non-specific sensitization of cattle body to tuberculin.

scholarly journals Prevalence of lip and perioral Ephelides in beach workers

2013 ◽  
Vol 88 (1) ◽  
pp. 56-63
Author(s):  
Eudes Euler de Souza Lucena ◽  
Ana Claudia de Queiroz Castro ◽  
Danielle Bezerra de Farias ◽  
Pérola Teixeira de Lima ◽  
Éricka Janine Dantas da Silveira ◽  
...  
Keyword(s):  
General Health ◽  
Rio Grande ◽  
Clinical Tests ◽  
Melanin Production ◽  
Chi Square ◽  
Sociodemographic Variables ◽  
Significance Level ◽  
Chi Square Test ◽  
Study Population ◽  
The City

BACKGROUND: Ephelides are small hyperpigmented macules common in the skin, presenting as areas with increased melanin production. Ephelides are observed in genetically predisposed individuals, particularly fair-skinned people highly susceptible to sunburn. OBJECTIVES: This study aimed to determine the prevalence of lip and perioral ephelides in 362 beach workers in the city of Natal (Rio Grande do Norte) and to investigate potential associations with sociodemographic, occupational and general health. METHODS: For this purpose clinical tests were performed by calibrated examiners in the epidemiological area around the lips and the area bounded laterally by the nasolabial groove and at the bottom by the chin. A questionnaire was completed and assessed. The possible associations between sociodemographic variables, occupational and general health with the presence of lip and perioral ephelides were evaluated by chi-square test for a significance level of 5%. RESULTS: Approximately one third of the workers were affected by perioral ephelides (33.7%) and around a quarter of them by lip ephelides (24.0%). Gender was the only variable significantly associated with the presence of perioral ephelides (p = 0.002), unlike lip ephelides which proved to be significantly associated with habits (p = 0.036) and alcoholism (0.030). CONCLUSIONS: The prevalence of ephelides in lip and perioral region was high in the study population, with gender and certain habits associated with its occurrence.

scholarly journals Improved Skin Test for Differential Diagnosis of Bovine Tuberculosis by the Addition of Rv3020c-Derived Peptides

2012 ◽  
Vol 19 (4) ◽  
pp. 620-622 ◽  
Author(s):  
Gareth J. Jones ◽  
Adam Whelan ◽  
Derek Clifford ◽  
Mick Coad ◽  
H. Martin Vordermeier
Keyword(s):  
Differential Diagnosis ◽  
Skin Test ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Johne's Disease ◽  
Diagnostic Sensitivity ◽  
Bacillus Calmette Guerin ◽  
Content Type ◽  
The Face ◽  
Mycobacterial Antigens

ABSTRACTA peptide cocktail derived from the mycobacterial antigens ESAT-6, CFP-10, and Rv3615c allowed differentiation betweenMycobacterium bovis-infected and M. bovis bacillus Calmette-Guérin (BCG)-vaccinated cattle when used as a skin test reagent for a “DIVA” test (i.e., a test capable ofdifferentiatinginfected and uninfectedvaccinatedanimals). Addition of the antigen Rv3020c improves the diagnostic sensitivity without compromising specificity in the face of BCG or Johne's disease vaccination.

scholarly journals Pycnodysostosis with Special Emphasis on Dentofacial Characteristics

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Aisha Khoja ◽  
Mubassar Fida ◽  
Attiya Shaikh
Keyword(s):  
Differential Diagnosis ◽  
Case Report ◽  
Bone Turnover ◽  
Autosomal Recessive ◽  
Autosomal Recessive Disorder ◽  
Great Emphasis ◽  
Treatment Goals ◽  
Oral Complications ◽  
Characteristic Features ◽  
Insight Into

Pycnodysostosis is an autosomal recessive disorder that manifests as osteosclerosis of the skeleton due to the defective osteoclasts mediated bone turnover. The diagnosis of this disorder is established on the basis of its characteristic features and must be differentially diagnosed with other bone disorders. Dental surgeons should be aware of the limitations and possible adverse oral complications such as osteomyelitis of bone in these patients. This will guide them in planning realistic treatment goals. This paper reports the clinical and radiographic features of pycnodysostosis with the great emphasis on its dentofacial characteristics. The aim of this case report is to give an insight into the etiology, pathogenesis, and differential diagnosis of this disorder and to prepare the dentists and maxillofacial surgeons to overcome the challenges in treating these patients.

scholarly journals Influence of the incubation conditions on culture media to optimize primary isolation of Mycobacterium bovis

2016 ◽  
Vol 37 (5Supl2) ◽  
pp. 3693
Author(s):  
Cássia Yumi Ikuta ◽  
Flávia Morato ◽  
Gisele Oliveira de Souza ◽  
Marcos Bryan Heinemann ◽  
Marcos Amaku ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Culture Media ◽  
Growth Time ◽  
Solid Culture ◽  
Sodium Pyruvate ◽  
Primary Isolation ◽  
Granulomatous Lesions ◽  
Tuberculosis Diagnosis ◽  
Carbon Dioxide Co2

The isolation of Mycobacterium bovis is critical to a surveillance system for bovine tuberculosis based on detection of lesions in abattoirs. Thus, four solid culture media and three incubation conditions were investigated to elucidate which combination overcomes the others by assessing growth, time to the first appearance of colonies and their number. Ninety-seven samples of granulomatous lesions were submitted to the decontamination procedure by 1-hexadecylpyridinium chloride at 0.75% w/v, and inoculated on two egg-based media, Stonebrink’s (ST) and Löwenstein-Jensen’s with sodium pyruvate (LJp), and two agar-based media, tuberculosis blood agar (B83) and Middlebrook 7H11 medium (7H11). Each medium was incubated at 37°C for 90 days in three incubation conditions: in air, in air containing 10% carbon dioxide (CO2), and in air in slopes closed with burned hydrophobic cotton and subsequently plugged with a cork to create a microaerophilic atmosphere. The colonies appeared faster and in higher number when incubated in air containing 10% CO2 (p < 0.01), independent of media. B83 showed a faster growth and detected more isolates at 30 days of incubation, when compared to ST (0.0178), LJp (p < 0.0001) and 7H11 (p < 0.0001), though there was no difference between B83, ST and LJp at 60 and 90 days of incubation. 7H11 presented the lowest number of isolates (p < 0.0001) and a longer period for the appearance of the first colony (p < 0.001). According to our findings, the concomitant use of ST and B83 media incubated in air containing 10% CO2 increases the isolation of M. bovis in a shorter period of time, which improves bovine tuberculosis diagnosis.

Korsakoff's syndrome on the MMPI-2

2002 ◽  
Vol 14 (5) ◽  
pp. 231-236 ◽  
Author(s):  
Jos I. M. Egger ◽  
Arie J. Wester ◽  
Hubert R. A. De Mey ◽  
Jan J. L. Derksen
Keyword(s):  
Differential Diagnosis ◽  
The Netherlands ◽  
Psychiatric Hospital ◽  
Exploratory Study ◽  
Chronic Alcoholism ◽  
Content Scales ◽  
Flat Profile ◽  
Insight Into ◽  
Korsakoff's Disease ◽  
Alcohol Dependent

Background:This article describes an exploratory study of MMPI-2 administration to 20 patients with Korsakoff's disease, all admitted to the Korsakoff unit of a major psychiatric hospital in the Netherlands.Methods:We compared their MMPI-2 basic profiles with those of an inpatient alcohol-dependent non-Korsakoff group. Attention was also given to content scales and some selected supplementary scales. Furthermore, we explored the differences between MMPI-2 profiles of Korsakoff patients with and without insight into oneself and one's disease.Results:Compared with both the Dutch norms and the scale means of the non-Korsakoff alcoholics, Korsakoff patients showed an extraordinary flat profile. Illness insight appeared to be related to the levels of the various scales.Discussion:The results show the potential usefulness of the MMPI-2 in the differential diagnosis of chronic alcoholism and Korsakoff's disease.

scholarly journals Diaskintest for the diagnosis of bovine tuberculosis

2021 ◽  
Vol 98 (12) ◽  
pp. 53-56
Author(s):  
A. Kh. Naymanov ◽  
A. M. Gulyukin ◽  
N. G. Tolstenko ◽  
E. P. Vangeli ◽  
V. M. Kalmykov
Keyword(s):  
Differential Diagnosis ◽  
Adverse Events ◽  
Bovine Tuberculosis ◽  
Guinea Pigs ◽  
Tuberculin Test ◽  
Diagnostic Value ◽  
Farm Animals ◽  
Laboratory Animals

The objective of the study: to compare the diagnostic value of intracutaneous tests with tuberculin and diaskintest for detection of tuberculosis in cattle.Subjects and methods. In this study, PPD tuberculin for mammals was used, it was made from M. bovis strain AN-5, manufactured by BIOK, and Diaskintest (recombinant tuberculous allergen, solution forintracutaneous administration) manufactured by ZAO FF Lecco, series 030307.Laboratory animals (guinea pigs, rabbits, chickens) and farm animals (pigs and cattle) were used in the study.Results. It has been established on laboratory animals that diaskintest does not cause any adverse events. When assessing sows sensitized with M. avium, it was found that the intracutaneous administration of PPD tuberculin to mammals resulted in up to 8.5% of positive reactions, while diaskintest was negative in all of them. Among cattle sensitized to non-tuberculous mycobacteria, intracutaneous administration of tuberculin for mammals revealed up to 4.6% of positive reactions, and diaskintest – up to 0.6%. In the farm with the unfavorable TB situation where animals infected with M. bovis were previously detected, when examining 177 cows, tuberculin test was positive in 102 (57.6%) of them, and diaskintest in 88 (49.7%). Diaskintest can be used for differential diagnosis of tuberculosis and sensitization by non-tuberculous mycobacteria in pigs and cattle.

Insights into the Design of p97-targeting Small Molecules from Structural Studies on p97 Functional Mechanism

2020 ◽  
Vol 27 (2) ◽  
pp. 298-316
Author(s):  
Xin Sui ◽  
Man Pan ◽  
Yi-Ming Li
Keyword(s):  
Small Molecules ◽  
Protein Unfolding ◽  
Molecular Mechanisms ◽  
Protein Quality ◽  
The Body ◽  
Clinical Tests ◽  
Subcellular Membrane ◽  
Aaa Protein ◽  
Insight Into ◽  
Lateral Sclerosis

p97, also known as valosin-containing protein or CDC48, is a member of the AAA+ protein family that is highly conserved in eukaryotes. It binds to various cofactors in the body to perform its protein-unfolding function and participates in DNA repair, degradation of subcellular membrane proteins, and protein quality control pathways, among other processes. Its malfunction can lead to many diseases, such as inclusion body myopathy, associated with Paget’s disease of bone and/or frontotemporal dementia, amyotrophic lateral sclerosis disease, and others. In recent years, many small-molecule inhibitors have been deployed against p97, including bis (diethyldithiocarbamate)- copper and CB-5083, which entered the first phase of clinical tests but failed. One bottleneck in the design of p97 drugs is that its molecular mechanism remains unclear. This paper summarizes recent studies on the molecular mechanisms of p97, which may lead to insight into how the next generation of small molecules targeting p97 can be designed.

Eosinophilic Pustular Folliculitis

PEDIATRICS ◽  
1992 ◽  
Vol 89 (6) ◽  
pp. 1095-1098
Author(s):  
GARY L. DARMSTADT ◽  
WALTER W. TUNNESSEN ◽  
RONALD J. SWEREN
Keyword(s):  
Differential Diagnosis ◽  
Effective Treatment ◽  
Clinical Experience ◽  
Pediatric Patients ◽  
Clinical Presentation ◽  
Clinical Entity ◽  
Treatment Modalities ◽  
First Report ◽  
Eosinophilic Infiltrate ◽  
Insight Into

Eosinophilic pustular folliculitis is a cutaneous disorder that consists of recurrent crops of pruritic, sterile, papulopustules in a follicular distribution. In pediatric patients, EPF presents primarily in the scalp and is confused with several other more common dermatoses in children. The diagnosis of EPF rests on its inclusion in the differential diagnosis of papulo-pustular disorders, the recognition of the clinical presentation, and the presence of an eosinophilic infiltrate on biopsy. Treatment with midpotency topical coticosteroids has thus far met with modest success. This is the first report on EPF in the pediatric literature. As pediatricians become more aware of EPF as a distinct clinical entity and as our clinical experience and insight into the pathogenesis of EPF grows, perhaps more effective treatment modalities will be devised.

An advanced intelligent ELISA test for bovine tuberculosis diagnosis

2018 ◽  
Vol 46 ◽  
pp. 59-66 ◽  
Author(s):  
Hanene Sahli ◽  
Aymen Mouelhi ◽  
Mohamed Fethi Diouani ◽  
Lotfi Tlig ◽  
Amira Refai ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Elisa Test ◽  
Tuberculosis Diagnosis
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