scholarly journals Non-tuberculous Mycobacteria in South African Wildlife: Neglected Pathogens and Potential Impediments for Bovine Tuberculosis Diagnosis

2017 ◽  
Vol 7 ◽  
Author(s):  
Nomakorinte Gcebe ◽  
Tiny M. Hlokwe
Keyword(s):  
South African ◽  
Bovine Tuberculosis ◽  
Tuberculosis Diagnosis

scholarly journals Influence of the incubation conditions on culture media to optimize primary isolation of Mycobacterium bovis

2016 ◽  
Vol 37 (5Supl2) ◽  
pp. 3693
Author(s):  
Cássia Yumi Ikuta ◽  
Flávia Morato ◽  
Gisele Oliveira de Souza ◽  
Marcos Bryan Heinemann ◽  
Marcos Amaku ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Culture Media ◽  
Growth Time ◽  
Solid Culture ◽  
Sodium Pyruvate ◽  
Primary Isolation ◽  
Granulomatous Lesions ◽  
Tuberculosis Diagnosis ◽  
Carbon Dioxide Co2

The isolation of Mycobacterium bovis is critical to a surveillance system for bovine tuberculosis based on detection of lesions in abattoirs. Thus, four solid culture media and three incubation conditions were investigated to elucidate which combination overcomes the others by assessing growth, time to the first appearance of colonies and their number. Ninety-seven samples of granulomatous lesions were submitted to the decontamination procedure by 1-hexadecylpyridinium chloride at 0.75% w/v, and inoculated on two egg-based media, Stonebrink’s (ST) and Löwenstein-Jensen’s with sodium pyruvate (LJp), and two agar-based media, tuberculosis blood agar (B83) and Middlebrook 7H11 medium (7H11). Each medium was incubated at 37°C for 90 days in three incubation conditions: in air, in air containing 10% carbon dioxide (CO2), and in air in slopes closed with burned hydrophobic cotton and subsequently plugged with a cork to create a microaerophilic atmosphere. The colonies appeared faster and in higher number when incubated in air containing 10% CO2 (p < 0.01), independent of media. B83 showed a faster growth and detected more isolates at 30 days of incubation, when compared to ST (0.0178), LJp (p < 0.0001) and 7H11 (p < 0.0001), though there was no difference between B83, ST and LJp at 60 and 90 days of incubation. 7H11 presented the lowest number of isolates (p < 0.0001) and a longer period for the appearance of the first colony (p < 0.001). According to our findings, the concomitant use of ST and B83 media incubated in air containing 10% CO2 increases the isolation of M. bovis in a shorter period of time, which improves bovine tuberculosis diagnosis.

An advanced intelligent ELISA test for bovine tuberculosis diagnosis

2018 ◽  
Vol 46 ◽  
pp. 59-66 ◽  
Author(s):  
Hanene Sahli ◽  
Aymen Mouelhi ◽  
Mohamed Fethi Diouani ◽  
Lotfi Tlig ◽  
Amira Refai ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Elisa Test ◽  
Tuberculosis Diagnosis

scholarly journals More on search for causes of sensitization to tuberculin PPD for mammals in cattle

2021 ◽  
pp. 271-276
Author(s):  
M. O. Baratov ◽  
P. S. Huseynova
Keyword(s):  
Differential Diagnosis ◽  
Bovine Tuberculosis ◽  
Nontuberculous Mycobacterium ◽  
Clinical Tests ◽  
Postmortem Diagnosis ◽  
Significance Level ◽  
Acid Tolerant ◽  
Tuberculosis Diagnosis ◽  
Trematode Infections ◽  
Insight Into

Despite the large number of papers dealing with the description of proposed methods for bovine tuberculosis diagnosis and mechanisms of non-specific reaction development in diseased and healthy animals, various aspects require further study. Many specialists are still of the view, formulated when studying causes of pseudoallergic reactions, that the agents of actinomycosis, trematode infections etc. can cause sensitization of the animal body to tuberculin PPD for mammals. The possibility of sensitization of cattle body to Аctinomyces bovis tuberculin was studied in 240 animals identified as actinomycosis diseased among 3,473 tested animals. Only 11 (4.6%) of the total number of diseased animals were reactors to tuberculin PPD for mammals. During bacteriological tests of material from animals euthanized for diagnostic purposes (tuberculin reactors and nonreactors with a confirmed postmortem diagnosis of actinomycosis), acid-tolerant nontuberculous mycobacterium (NTM) cultures were isolated. The results of the experiment conducted in 628 cows of a dairy holding confirmed that Actinomyces bovis lacks tuberculin-associated allergenicity: actinomycosis was detected only in one of 96 (15.2%) tuberculin reactors. The conducted clinical tests with high significance level (Р < 0.005) showed that there is no association between allergic reaction to tuberculin PPD for mammals and actinomycosis infection. The obtained results are indicative of imperfections in differential diagnosis, and further studies in this field should significantly contribute to gaining a better insight into non-specific sensitization of cattle body to tuberculin.

scholarly journals Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis

Tuberculosis ◽  
2015 ◽  
Vol 95 (6) ◽  
pp. 795-801 ◽  
Author(s):  
María E. Eirin ◽  
Analia Macias ◽  
Gabriel Magnano ◽  
Claudia Morsella ◽  
Laura Mendez ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Interferon Gamma ◽  
Bovine Tuberculosis ◽  
Interferon Gamma Release Assay ◽  
Release Assay ◽  
Tuberculosis Diagnosis

scholarly journals Review of Diagnostic Tests for Detection of Mycobacterium bovis Infection in South African Wildlife

2021 ◽  
Vol 8 ◽  
Author(s):  
Netanya Bernitz ◽  
Tanya J. Kerr ◽  
Wynand J. Goosen ◽  
Josephine Chileshe ◽  
Roxanne L. Higgitt ◽  
...  
Keyword(s):  
South Africa ◽  
South African ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Diagnostic Tests ◽  
Clinical Disease ◽  
Diagnostic Tools ◽  
Wildlife Species ◽  
Conservation Concern ◽  
And Control

Wildlife tuberculosis is a major economic and conservation concern globally. Bovine tuberculosis (bTB), caused by Mycobacterium bovis (M. bovis), is the most common form of wildlife tuberculosis. In South Africa, to date, M. bovis infection has been detected in 24 mammalian wildlife species. The identification of M. bovis infection in wildlife species is essential to limit the spread and to control the disease in these populations, sympatric wildlife species and neighboring livestock. The detection of M. bovis-infected individuals is challenging as only severely diseased animals show clinical disease manifestations and diagnostic tools to identify infection are limited. The emergence of novel reagents and technologies to identify M. bovis infection in wildlife species are instrumental in improving the diagnosis and control of bTB. This review provides an update on the diagnostic tools to detect M. bovis infection in South African wildlife but may be a useful guide for other wildlife species.

scholarly journals Comparison between tests for tuberculosis diagnosis in slaughtered bovines

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
David Attuy Vey da Silva ◽  
Márcio Junio Lima Siconelli ◽  
Karina Paes Bürger ◽  
Lara Borges Keid
Keyword(s):  
Bovine Tuberculosis ◽  
Dna Detection ◽  
Bacterial Isolation ◽  
Solid Culture ◽  
Chain Reaction ◽  
Bacterial Dna ◽  
Tuberculosis Diagnosis ◽  
Polymerase Chain ◽  
Low Sensitivity ◽  
Different Sources

ABSTRACT: Our goal for this article is to compare several different diagnosis tests for bovine tuberculosis identification. We have performed bacterial isolation, histopathological characterization, acid-fast bacilli (AFB) identification and M. bovis DNA detection. Lesions suggestive of Tuberculosis were sampled from bovine lymph nodes during slaughtering of bovines at an abattoir that operates under federal inspection. The bacterial isolation was performed in solid culture mediums, the histopathological characterization was made by Hematoxylin-eosinstaining, and AFB identification by Ziehl-Neelsen staining. Bacterial DNA detection was performed by Polymerase Chain Reaction (PCR) using DNA from two different sources, directly collected from the tuberculosis-like lesions (PCR followed by nested PCR) and from isolated bacteria. We have concluded that the multi-step approach, including histopathological characterization, bacterial isolation and AFB identification, is strongly recommended to diagnose tuberculosis in bovines. Furthermore, PCR assays using specimens of lesions suggestive of tuberculosis are a faster and more promising way to diagnose the disease. However, it should not be used alone due to the low sensitivity shown in this study.

scholarly journals Assessment of gross lesions preservation methods for the direct diagnosis of bovine tuberculosis: a constant search for improvement

2021 ◽  
Author(s):  
Nicolás Céspedes Cárdenas ◽  
Cássia Yumi Ikuta ◽  
Vanessa Cristinne Victor Rabaquim ◽  
Antonio Francisco Souza Filho ◽  
Gisele Oliveira Souza ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Sodium Borate ◽  
Indirect Contact ◽  
Fta Cards ◽  
Preservation Method ◽  
Solution Methods ◽  
Gross Lesions ◽  
Tuberculosis Diagnosis ◽  
Environmental Temperatures ◽  
Borate Solution

Abstract Objectives The present study assessed the sensitivity and biosafety of the FTA Elute Card® (FTA) (GE Healthcare) as an alternative preservation method of gross lesions for bovine tuberculosis diagnosis, that can endure large distances and high environmental temperatures, which are found in the Brazilian territory. Results The FTA card was compared to the freezing and sodium borate solution methods to preserve 134 gross lesions samples from slaughterhouses located in three Brazilian states. The calculated value for the sensitivity of sodium borate solution (SBS) stored for 30 days was higher than those estimated for the methods of freezing and SBS stored for 60 days, when the PCR and isolation results were interpreted in parallel (0.55). The FTA cards presented the lowest diagnostic sensitivity and did not inactivate all mycobacteria. Given the rush of a slaughterhouse routine, the quantity of lesions with different pathological stages cannot be measured on each FTA card. Thus, as a preservation method of gross tuberculosis lesions, the FTA cards can present infection risks for people who have direct or indirect contact with them.

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