The expression of constitutively active CPK3 impairs potassium uptake and transport in Arabidopsis under low K+ stress

Cell Calcium ◽  
2021 ◽  
pp. 102447
Author(s):  
Ren Huimin ◽  
Jamshaid Hussain ◽  
Li Wenjie ◽  
Yao Fenyong ◽  
Guo Junjun ◽  
...  
1964 ◽  
Vol 15 (3) ◽  
pp. 413-421 ◽  
Author(s):  
D. J. F. BOWLING ◽  
P. E. WEATHERLEY

1968 ◽  
Vol 52 (3) ◽  
pp. 424-443 ◽  
Author(s):  
Clifford L. Slayman ◽  
Carolyn W. Slayman

Net uptake of potassium by low K, high Na cells of Neurospora at pH 5.8 is accompanied by net extrusion of sodium and hydrogen ions. The amount of potassium taken up by the cells is matched by the sum of sodium and hydrogen ions lost, under a variety of conditions: prolonged preincubation, partial respiratory inhibition (DNP), and lowered [K]o. All three fluxes are exponential with time and obey Michaelis kinetics as functions of [K]o. The Vmax for net potassium uptake, 22.7 mmoles/kg cell water/min, is very close to that for K/K exchange reported previously (20 mmoles/kg cell water/min). However, the apparent Km for net potassium uptake, 11.8 mM [K]o, is an order of magnitude larger than the value (1 mM) for K/K exchange. It is suggested that a single transport system handles both net K uptake and K/K exchange, but that the affinity of the external site for potassium is influenced by the species of ion being extruded.


mBio ◽  
2013 ◽  
Vol 4 (5) ◽  
Author(s):  
Angelika Gründling

ABSTRACTStaphylococcus aureusis a hardy organism that can survive high-salt conditions better than many other bacteria. This characteristic is thought to helpS. aureussurvive in the nares and on the skin of the human host and is used to selectively propagate and identifyStaphylococcusspecies. However, the mechanism that allowsS. aureusto tolerate such high-salt conditions is not well understood. A recent study inmBioby A. Price-Whelan et al. [mBio 4(4):e00407-13, 2013, doi:10.1128/mBio.00407-13] highlights the importance of potassium uptake in this process. This commentary provides a perspective of the study by Price-Whelan et al. as well as other recently reported work on potassium uptake and transport systems inS. aureus.


2019 ◽  
Vol 5 (1) ◽  
pp. 10-16 ◽  
Author(s):  
Jianguo Qi ◽  
Simin Sun ◽  
Lin Yang ◽  
Mingjun Li ◽  
Fengwang Ma ◽  
...  

Author(s):  
William P. Wergin ◽  
P. F. Bell ◽  
Rufus L. Chaney

In dicotyledons, Fe3+ must be reduced to Fe2+ before uptake and transport of this essential macronutrient can occur. Ambler et al demonstrated that reduction along the root could be observed by the formation of a stain, Prussian blue (PB), Fe4 [Fe(CN)6]3 n H2O (where n = 14-16). This stain, which is an insoluble precipitate, forms at the reduction site when the nutrient solution contains Fe3+ and ferricyanide. In 1972, Chaney et al proposed a model which suggested that the Fe3+ reduction site occurred outside the cell membrane; however, no physical evidence to support the model was presented at that time. A more recent study using the PB stain indicates that rapid reduction of Fe3+ occurs in a region of the root containing young root hairs. Furthermore the most pronounced activity occurs in plants that are deficient in Fe. To more precisely localize the site of Fe3+ reduction, scanning electron microscopy (SEM), x-ray analysis, and transmission electron microscopy (TEM) were utilized to examine the distribution of the PB precipitate that was induced to form in roots.


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